In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_12 ( 2015-12-01), p. 4580-4588
Abstract:
Culture-based study of the faecal microbiome in two adult female subjects revealed the presence of two obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gram-negative bacterial strains that represent novel species. The first strain, designated 627 T , was a fastidious, slow-growing, indole-positive bacterium with a non-fermentative type of metabolism. The strain was characterized by the production of acetic and succinic acids as metabolic end products, the prevalence of iso-C 15 : 0 fatty acid and the presence of menaquinones MK-10 and MK-11. The DNA G+C content was found to be 56.6 mol%. The second strain, designated 177 T , was capable of fermenting a rich collection of carbohydrate substrates, producing acetic acid as a terminal product. The strain was indole-negative and resistant to bile. The major cellular fatty acids were iso-C 15 : 0 and anteiso-C 15 : 0 (in a 1 : 1 ratio) and the predominant menaquinone was MK-11. The DNA G+C content was 37.8 mol%. A phylogenomic analysis of the draft genomes of strains 627 T and 177 T placed these bacteria in the genera Alistipes (family Rikenellaceae ) and Coprobacter (family Porphyromonadaceae ), respectively. On the basis of the phenotypic and genotypic properties of strains 627 T and 177 T , we conclude that these strains from human faeces represent two novel bacterial species, for which the names Alistipes inops sp. nov. (type strain 627 T = DSM 28863 T = VKM B-2859 T ) and Coprobacter secundus sp. nov. (type strain 177 T = DSM 28864 T = VKM B-2857 T ) are proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.000617
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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