In:
PLOS Biology, Public Library of Science (PLoS), Vol. 18, No. 11 ( 2020-11-24), p. e3000965-
Abstract:
Near-infrared (NIR) genetically encoded calcium ion (Ca 2+ ) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report 2 improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca 2+ dynamics.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3000965
DOI:
10.1371/journal.pbio.3000965.g001
DOI:
10.1371/journal.pbio.3000965.g002
DOI:
10.1371/journal.pbio.3000965.g003
DOI:
10.1371/journal.pbio.3000965.g004
DOI:
10.1371/journal.pbio.3000965.g005
DOI:
10.1371/journal.pbio.3000965.s001
DOI:
10.1371/journal.pbio.3000965.s002
DOI:
10.1371/journal.pbio.3000965.s003
DOI:
10.1371/journal.pbio.3000965.s004
DOI:
10.1371/journal.pbio.3000965.s005
DOI:
10.1371/journal.pbio.3000965.s006
DOI:
10.1371/journal.pbio.3000965.s007
DOI:
10.1371/journal.pbio.3000965.s008
DOI:
10.1371/journal.pbio.3000965.s009
DOI:
10.1371/journal.pbio.3000965.s010
DOI:
10.1371/journal.pbio.3000965.s011
DOI:
10.1371/journal.pbio.3000965.s012
DOI:
10.1371/journal.pbio.3000965.s013
DOI:
10.1371/journal.pbio.3000965.s014
DOI:
10.1371/journal.pbio.3000965.r001
DOI:
10.1371/journal.pbio.3000965.r002
DOI:
10.1371/journal.pbio.3000965.r003
DOI:
10.1371/journal.pbio.3000965.r004
DOI:
10.1371/journal.pbio.3000965.r005
DOI:
10.1371/journal.pbio.3000965.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2126773-X
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