In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 2 ( 2023-2-24), p. e0280930-
Abstract:
Antibodies are important reagents for research, diagnostics, and therapeutics. Many examples of chimeric proteins combining the specific target recognition of antibodies with complementing functionalities such as fluorescence, toxicity or enzymatic activity have been described. However, antibodies selected solely on the basis of their binding specificities are not necessarily ideal candidates for the construction of chimeras. Here, we describe a high throughput method based on yeast display to directly select antibodies most suitable for conversion to fluorescent chimera. A library of scFv binders was converted to a fluorescent chimeric form, by cloning thermal green protein into the linker between VH and VL, and directly selecting for both binding and fluorescent functionality. This allowed us to directly identify antibodies functional in the single chain TGP format, that manifest higher protein expression, easier protein purification, and one-step binding assays.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0280930
DOI:
10.1371/journal.pone.0280930.g001
DOI:
10.1371/journal.pone.0280930.g002
DOI:
10.1371/journal.pone.0280930.g003
DOI:
10.1371/journal.pone.0280930.g004
DOI:
10.1371/journal.pone.0280930.g005
DOI:
10.1371/journal.pone.0280930.g006
DOI:
10.1371/journal.pone.0280930.g007
DOI:
10.1371/journal.pone.0280930.t001
DOI:
10.1371/journal.pone.0280930.t002
DOI:
10.1371/journal.pone.0280930.t003
DOI:
10.1371/journal.pone.0280930.t004
DOI:
10.1371/journal.pone.0280930.t005
DOI:
10.1371/journal.pone.0280930.s001
DOI:
10.1371/journal.pone.0280930.s002
DOI:
10.1371/journal.pone.0280930.s003
DOI:
10.1371/journal.pone.0280930.r001
DOI:
10.1371/journal.pone.0280930.r002
DOI:
10.1371/journal.pone.0280930.r003
DOI:
10.1371/journal.pone.0280930.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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