In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2023-10-2), p. e0011674-
Abstract:
A promising candidate for arbovirus control and prevention relies on replacing arbovirus-susceptible Aedes aegypti populations with mosquitoes that have been colonized by the intracellular bacterium Wolbachia and thus have a reduced capacity to transmit arboviruses. This reduced capacity to transmit arboviruses is mediated through a phenomenon referred to as pathogen blocking. Pathogen blocking has primarily been proposed as a tool to control dengue virus (DENV) transmission, however it works against a range of viruses, including Zika virus (ZIKV). Despite years of research, the molecular mechanisms underlying pathogen blocking still need to be better understood. Here, we used RNA-seq to characterize mosquito gene transcription dynamics in Ae . aegypti infected with the w Mel strain of Wolbachia that are being released by the World Mosquito Program in Medellín, Colombia. Comparative analyses using ZIKV-infected, uninfected tissues, and mosquitoes without Wolbachia revealed that the influence of w Mel on mosquito gene transcription is multifactorial. Importantly, because Wolbachia limits, but does not completely prevent, replication of ZIKV and other viruses in coinfected mosquitoes, there is a possibility that these viruses could evolve resistance to pathogen blocking. Therefore, to understand the influence of Wolbachia on within-host ZIKV evolution, we characterized the genetic diversity of molecularly barcoded ZIKV virus populations replicating in Wolbachia -infected mosquitoes and found that within-host ZIKV evolution was subject to weak purifying selection and, unexpectedly, loose anatomical bottlenecks in the presence and absence of Wolbachia . Together, these findings suggest that there is no clear transcriptional profile associated with Wolbachia -mediated ZIKV restriction, and that there is no evidence for ZIKV escape from this restriction in our system.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0011674
DOI:
10.1371/journal.pntd.0011674.g001
DOI:
10.1371/journal.pntd.0011674.g002
DOI:
10.1371/journal.pntd.0011674.g003
DOI:
10.1371/journal.pntd.0011674.g004
DOI:
10.1371/journal.pntd.0011674.g005
DOI:
10.1371/journal.pntd.0011674.g006
DOI:
10.1371/journal.pntd.0011674.g007
DOI:
10.1371/journal.pntd.0011674.g008
DOI:
10.1371/journal.pntd.0011674.s001
DOI:
10.1371/journal.pntd.0011674.s002
DOI:
10.1371/journal.pntd.0011674.s003
DOI:
10.1371/journal.pntd.0011674.s004
DOI:
10.1371/journal.pntd.0011674.s005
DOI:
10.1371/journal.pntd.0011674.s006
DOI:
10.1371/journal.pntd.0011674.s007
DOI:
10.1371/journal.pntd.0011674.s008
DOI:
10.1371/journal.pntd.0011674.s009
DOI:
10.1371/journal.pntd.0011674.s010
DOI:
10.1371/journal.pntd.0011674.s011
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2429704-5
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