In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 43, No. 12 ( 2005-12), p. 5888-5898
Abstract:
Campylobacter
fetus can be divided into the subspecies C. fetus subsp. fetus and C. fetus subsp. venerealis. C. fetus subsp. fetus causes
sporadic infections in humans and abortion in cattle and sheep and has been isolated from a variety of sites in different hosts. C.
fetus subsp. venerealis is host restricted, being
isolated mainly from the genital tracts of cattle, and is the causative agent of bovine genital campylobacteriosis. Despite differences in
niche preference, microbiological subspecies differentiation has proven difficult. Different typing methods divided C. fetus isolates
into different subgroups, depending on the methods used. The relative value of these methods can be assessed by the evolutionary relationship
of isolates belonging to the genus; therefore, we developed a multilocus sequence typing (MLST) scheme for C. fetus . This
scheme was applied to 140 C. fetus isolates previously typed
by amplified fragment length polymorphism (AFLP) analysis. A total of 14 different sequence types (STs) were identified, and these exhibited
low levels of inter-ST genetic diversity, with only 22 variable sites in 3,312 nucleotides. These MLST data indicate that C. fetus is genetically homogeneous compared to the homogeneity of other Campylobacter species. The two C. fetus subspecies
were extremely closely related genetically, but ST-4 was associated only with C. fetus subsp. venerealis , which
represents a “bovine” clone. The C. fetus subsp. fetus isolates studied were more diverse in terms of
their STs, and the STs correlated with epidemiological relationships. Congruence was observed among C. fetus subspecies, sap type, and ST; therefore, MLST confirms that mammalian C. fetus is genetically stable, probably as result of the
introduction of a single ancestral clone into a mammalian niche.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/JCM.43.12.5888-5898.2005
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2005
detail.hit.zdb_id:
1498353-9
SSG:
12
Permalink