In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 3 ( 2022-3-24), p. e1010438-
Abstract:
Regulated microneme secretion governs motility, host cell invasion and egress in the obligate intracellular apicomplexans. Intracellular calcium oscillations and phospholipid dynamics critically regulate microneme exocytosis. Despite its importance for the lytic cycle of these parasites, molecular mechanistic details about exocytosis are still missing. Some members of the P4-ATPases act as flippases, changing the phospholipid distribution by translocation from the outer to the inner leaflet of the membrane. Here, the localization and function of the repertoire of P4-ATPases was investigated across the lytic cycle of Toxoplasma gondii . Of relevance, ATP2B and the non-catalytic subunit cell division control protein 50.4 (CDC50.4) form a stable heterocomplex at the parasite plasma membrane, essential for microneme exocytosis. This complex is responsible for flipping phosphatidylserine, which presumably acts as a lipid mediator for organelle fusion with the plasma membrane. Overall, this study points toward the importance of phosphatidylserine asymmetric distribution at the plasma membrane for microneme exocytosis.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010438
DOI:
10.1371/journal.ppat.1010438.g001
DOI:
10.1371/journal.ppat.1010438.g002
DOI:
10.1371/journal.ppat.1010438.g003
DOI:
10.1371/journal.ppat.1010438.g004
DOI:
10.1371/journal.ppat.1010438.g005
DOI:
10.1371/journal.ppat.1010438.s001
DOI:
10.1371/journal.ppat.1010438.s002
DOI:
10.1371/journal.ppat.1010438.s003
DOI:
10.1371/journal.ppat.1010438.s004
DOI:
10.1371/journal.ppat.1010438.s005
DOI:
10.1371/journal.ppat.1010438.s006
DOI:
10.1371/journal.ppat.1010438.s007
DOI:
10.1371/journal.ppat.1010438.r001
DOI:
10.1371/journal.ppat.1010438.r002
DOI:
10.1371/journal.ppat.1010438.r003
DOI:
10.1371/journal.ppat.1010438.r004
DOI:
10.1371/journal.ppat.1010438.r005
DOI:
10.1371/journal.ppat.1010438.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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