In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 2 ( 2022-2-22), p. e3001528-
Abstract:
Bacteria are powerful models for understanding how cells divide and accomplish global regulatory programs. In Caulobacter crescentus , a cascade of essential master regulators supervises the correct and sequential activation of DNA replication, cell division, and development of different cell types. Among them, the response regulator CtrA plays a crucial role coordinating all those functions. Here, for the first time, we describe the role of a novel factor named CcnA (cell cycle noncoding RNA A), a cell cycle–regulated noncoding RNA (ncRNA) located at the origin of replication, presumably activated by CtrA, and responsible for the accumulation of CtrA itself. In addition, CcnA may be also involved in the inhibition of translation of the S-phase regulator, GcrA, by interacting with its 5′ untranslated region (5′ UTR). Performing in vitro experiments and mutagenesis, we propose a mechanism of action of CcnA based on liberation ( ctrA ) or sequestration ( gcrA ) of their ribosome-binding site (RBS). Finally, its role may be conserved in other alphaproteobacterial species, such as Sinorhizobium meliloti , representing indeed a potentially conserved process modulating cell cycle in Caulobacterales and Rhizobiales .
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001528
DOI:
10.1371/journal.pbio.3001528.g001
DOI:
10.1371/journal.pbio.3001528.g002
DOI:
10.1371/journal.pbio.3001528.g003
DOI:
10.1371/journal.pbio.3001528.g004
DOI:
10.1371/journal.pbio.3001528.g005
DOI:
10.1371/journal.pbio.3001528.g006
DOI:
10.1371/journal.pbio.3001528.g007
DOI:
10.1371/journal.pbio.3001528.g008
DOI:
10.1371/journal.pbio.3001528.g009
DOI:
10.1371/journal.pbio.3001528.s001
DOI:
10.1371/journal.pbio.3001528.s002
DOI:
10.1371/journal.pbio.3001528.s003
DOI:
10.1371/journal.pbio.3001528.s004
DOI:
10.1371/journal.pbio.3001528.s005
DOI:
10.1371/journal.pbio.3001528.s006
DOI:
10.1371/journal.pbio.3001528.s007
DOI:
10.1371/journal.pbio.3001528.s008
DOI:
10.1371/journal.pbio.3001528.s009
DOI:
10.1371/journal.pbio.3001528.s010
DOI:
10.1371/journal.pbio.3001528.s011
DOI:
10.1371/journal.pbio.3001528.s012
DOI:
10.1371/journal.pbio.3001528.s013
DOI:
10.1371/journal.pbio.3001528.s014
DOI:
10.1371/journal.pbio.3001528.s015
DOI:
10.1371/journal.pbio.3001528.s016
DOI:
10.1371/journal.pbio.3001528.s017
DOI:
10.1371/journal.pbio.3001528.s018
DOI:
10.1371/journal.pbio.3001528.s019
DOI:
10.1371/journal.pbio.3001528.s020
DOI:
10.1371/journal.pbio.3001528.s021
DOI:
10.1371/journal.pbio.3001528.s022
DOI:
10.1371/journal.pbio.3001528.s023
DOI:
10.1371/journal.pbio.3001528.s024
DOI:
10.1371/journal.pbio.3001528.s025
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
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