In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-21), p. e0248607-
Abstract:
Microbial colonization of plant roots is a highly complex process that requires the coordination and regulation of many gene networks, yet the identities and functions of many of these gene products have yet to be discovered. Pantoea sp. YR343, a gamma-proteobacterium isolated from the rhizosphere of Populus deltoides , forms robust biofilms along the root surfaces of Populus and possesses plant growth-promoting characteristics. In this work, we identified three diguanylate cyclases in the plant-associated microbe Pantoea sp. YR343 that are expressed in the presence of plant roots. One of these diguanylate cyclases, DGC2884, localizes to discrete sites in the cells and its overexpression results in reduced motility and increased EPS production and biofilm formation. We performed a genetic screen by expressing this diguanylate cyclase from an inducible promoter in order to identify candidate gene products that may be involved in root colonization by Pantoea sp. YR343. Further, we demonstrate the importance of other domains in DGC2884 to its activity, which in combination with the genes identified by transposon mutagenesis, may yield insights into the mechanisms of plant association as well as the activity and regulation of homologous enzymes in medically and agriculturally relevant microbes.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0248607
DOI:
10.1371/journal.pone.0248607.g001
DOI:
10.1371/journal.pone.0248607.g002
DOI:
10.1371/journal.pone.0248607.g003
DOI:
10.1371/journal.pone.0248607.g004
DOI:
10.1371/journal.pone.0248607.g005
DOI:
10.1371/journal.pone.0248607.t001
DOI:
10.1371/journal.pone.0248607.t002
DOI:
10.1371/journal.pone.0248607.t003
DOI:
10.1371/journal.pone.0248607.s001
DOI:
10.1371/journal.pone.0248607.s002
DOI:
10.1371/journal.pone.0248607.s003
DOI:
10.1371/journal.pone.0248607.s004
DOI:
10.1371/journal.pone.0248607.s005
DOI:
10.1371/journal.pone.0248607.s006
DOI:
10.1371/journal.pone.0248607.s007
DOI:
10.1371/journal.pone.0248607.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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