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  • 1
    Online Resource
    Online Resource
    Purification and Biochemical Properties of Acid Phosphatase Enzyme from Seedlings of Rumex dentatus (Curly dock)
    Chemical Society of Pakistan ; 2019
    In:  Journal of the chemical society of pakistan Vol. 41, No. 6 ( 2019), p. 1065-1065
    Details
    In: Journal of the chemical society of pakistan, Chemical Society of Pakistan, Vol. 41, No. 6 ( 2019), p. 1065-1065
    Abstract: The study was designed to purify enzyme from weed plant in association with wheat crop to assess and compare the purification and biochemical properties with acid phosphatases of seedlings of various plants. Acid phosphatase from seedlings of Rumex dentatus (Curly dock) was purified by different chromatography and chromatofocusing techniques with specific activity of 63U/mg of protein. The yield was about 3%. Single band was detected on SDS-polyacrylamide gel electrophoresis confirming the enzyme was homogeneous. Apparent molecular weight of 48 kDa was obtained. Gel filtration experiment indicated that native enzyme had a approximately molecular weight of 96 kDa, suggesting that this enzyme is homodimer. The enzyme showed Km value of 0.5 mM and Vmax of 60 and#181;M of p-nitrophenyl phosphate hydrolysed/min/mg of protein under experimental conditions. The optimum pH of 5.0 and temperature of 50and#176;C were obtained. Divalent cations such as copper and zinc ions caused acid phosphatase inhibition but the presence of 20 mM EDTA in the enzyme-metal ions incubation mixtures reversed the enzyme inhibition to some extent. The activity of 54 % and 63% were recovered back, respectively. Ca2+ and Mg2+ had very small activating effect on activity in the absence or presence of EDTA. The reaction of enzyme with iodoacetic acid or N-ethyl-maleimide had no inhibitory effect, pointing to a non-involvement of cysteine residues in enzyme action. Further, β-mercaptoethanol or dithiothreitol at low concentrations had very little activating effect revealing that SH-group containing amino acid in the enzyme may not be significant for its catalytic activity. The pH dependent variation of Km study showed that histidine may constitute a part of the active site. Acid phosphatase was competitively inhibited by phosphate and vanadate. Fluoride and Zn2+ acted as non-competitive inhibitors while molybdate showed mixed type of inhibition.
    Type of Medium: Online Resource
    ISSN: 0253-5106
    Uniform Title: Purification and Biochemical Properties of Acid Phosphatase Enzyme from Seedlings of Rumex dentatus (Curly dock)
    URL: Article
    DOI: 10.52568/000827/JCSP/41.06.2019
    Language: Unknown
    Publisher: Chemical Society of Pakistan
    Publication Date: 2019
    detail.hit.zdb_id: 3024078-5
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  • 2
    Online Resource
    Online Resource
    Nutrient intake, nitrogen balance and growth performance in buffalo calves fed citrus pulp as a concentrate source
    Academic Journals ; 2016
    In:  African Journal of Agricultural Research Vol. 11, No. 29 ( 2016-07-21), p. 2562-2568
    Details
    In: African Journal of Agricultural Research, Academic Journals, Vol. 11, No. 29 ( 2016-07-21), p. 2562-2568
    Type of Medium: Online Resource
    ISSN: 1991-637X
    Uniform Title: English
    URL: Journal
    URL: Article
    DOI: 10.5897/AJAR
    DOI: 10.5897/AJAR2016.10900
    Language: Unknown
    Publisher: Academic Journals
    Publication Date: 2016
    detail.hit.zdb_id: 2378337-0
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  • 3
    Online Resource
    Online Resource
    Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida
    MDPI AG ; 2022
    In:  Molecules Vol. 27, No. 14 ( 2022-07-18), p. 4582-
    Details
    In: Molecules, MDPI AG, Vol. 27, No. 14 ( 2022-07-18), p. 4582-
    Abstract: In the current study, the anti-inflammatory and analgesic potential of Alnus nitida (leaves and fruits) was evaluated in the Sprague-Dawley rat. Traditionally, A. nitida was used for the treatment of inflammatory ailments. However, A. nitida leaves and fruits have not been yet reported regarding any potential medicinal effects. Leaves/fruits of A. nitida were extracted with methanol and fractionated to attain n-hexane, chloroform, ethyl acetate and aqueous fractions. These extracts were then evaluated for in vivo analgesic and anti-inflammatory potential. For in vivo anti-inflammatory activity, carrageenan-induced paw edema assay, Freunds’ complete adjuvant-induced edema, xylene-induced ear edema and histamine-induced paw edema models were used in rats, which showed significant (p 〈 0.01) reduction (70–80%) in edema in comparison of inflammatory controls. On other hand, for the analgesic assessment, hot plate assay and acetic acid-induced writhing tests were used, which showed a significant (p 〈 0.01) rise in latency time (40–60%) as compared with pain-induced controls. These results were comparable with standard drugs in a concentration-dependent manner and no mortality or toxicity was observed during all experiments. Then, for the identification of chemical constituents gas chromatography–mass spectrometry (GC-MS) analysis was performed, which indicated the presence of neophytadiene, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, phytol and vitamin E, justifying the use of A. nitida to treat inflammatory disorders.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules27144582
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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  • 4
    Online Resource
    Online Resource
    EVALUATING THE PHYTOTOXIC EFFECTS OF SOME PLANT SPECIES OF SEMI-ARID REGIONS
    IK Press ; 2022
    In:  PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY ( 2022-01-05), p. 1-18
    Details
    In: PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, IK Press, ( 2022-01-05), p. 1-18
    Abstract: The most effective method for weed control is the use of herbicides. However, the scope of this method is narrowing down due to herbicide-resistant weeds and the non-availability of new chemistry herbicides. Hence, to explore natural weed management options, the herbicidal potential of three weed species extracts namely Opuntia ficus-indica, Murraya koenigii and Dryopteris felix-mas were evaluated in lab and wirehouse experiments. A completely randomized design with factorial arrangements was used for lab and completely randomized design was used for wirehouse experiments. Each experiment was repeated twice with four replications each time. Data pertaining to seed germination, seedling growth and physiological traits of crops and weeds were recorded using standard procedures. Data indicated that aqueous extracts of tested species depicted more phytotoxicity than N-hexane extracts against germination and seedling growth of lettuce in the lab experiment. Aqueous extract of Opuntia ficus-indica completely inhibited lettuce seed germination whereas, N-hexane extract of the same species has least effect on germination and germination traits like mean germination time, time taken to 50% germination, germination index. The aqueous extract of O. ficus-indica resulted in minimum lentil, mashbean, mungbean, parthenium and Trianthema portuclacastrum seedling leaf area, chlorophyll content, shoot, root length, dry weight and maximum electrolyte leakage. The foliar application of O. ficus-indica, M. koenigii and D. felix-mas water extracts on seedlings of lentil, mashbean, mungbean, parthenium and T. portuclacastrum followed the phyto-toxicity order O. ficus-indica aqueous extract ˃ D. felix-mas aqueous extract ˃ M. koenigii aqueous extract. The growth-inhibitory demonstrations of O. ficus-indica extract suggest that active substances with herbicidal properties are present in this plant which could be exploited as natural weedicide.
    Type of Medium: Online Resource
    ISSN: 0972-2025
    URL: Article
    DOI: 10.56557/pcbmb/2022/v23i1-27376
    Language: Unknown
    Publisher: IK Press
    Publication Date: 2022
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  • 5
    Online Resource
    Online Resource
    Prevalence of Diabetes Type 2 in Hepatitis C Infected Patients in Kpk, Pakistan
    Hindawi Limited ; 2017
    In:  BioMed Research International Vol. 2017 ( 2017), p. 1-4
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2017 ( 2017), p. 1-4
    Abstract: Hepatitis C (HCV) and diabetes mellitus are the two main health concerns that cause devastating health and financial worries worldwide. It has been observed in the past that both diseases have a high correlation that might be due to the abnormal conditions of the liver. But the mechanism of the prevalence of diabetes in patients with chronic HCV infection still remains unclear. In our study, we have investigated T2DM in the male and female patients at Lady Reading Hospital (LRH), Peshawar. The blood samples of both in- and outpatients were analysed in the PCR laboratories of LRH from December 2014 to April 2015. Great prevalence of diabetes in hepatitis C infected male and female patients was observed during this study. The data were collected from the patients through a preplanned questionnaire that included name of the patient, HCV, being diabetic, age, gender, location, educational background, family history of the disease, other diseases, and any treatments if taken. The results of our study have found 26.42% prevalence of T2DM in HCV infected patients. So we conclude that HCV infection may be one of the reasons that could lead to T2DM.
    Type of Medium: Online Resource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2017/2416281
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2017
    detail.hit.zdb_id: 2698540-8
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  • 6
    Online Resource
    Online Resource
    Effects of Phoenix dactylifera against Streptozotocin-Aluminium Chloride Induced Alzheimer’s Rats and Their In Silico Study
    Hindawi Limited ; 2023
    In:  BioMed Research International Vol. 2023 ( 2023-1-9), p. 1-21
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2023 ( 2023-1-9), p. 1-21
    Abstract: Phoenix dactylifera is known for medicinal importance due to its antioxidant, antidiabetic, antidepressant, and anti-inflammatory properties. This study is aimed at evaluating the effect of P. dactylifera seeds to cure Alzheimer’s disease (AD). AD was induced in the rats with streptozotocin + aluminium chloride followed by treatment of methanolic extract of P. dactylifera seeds. The blood glucose levels were determined at regular intervals, which showed a prominent decrease in the extracts treated group. Behavior tests, including the Elevated Plus Maze (EPM) test and Morris Water Maze (MWM) test, were used to evaluate memory patterns in rats. The results indicated that extract-treated rats significantly improved memory behavior compared to the diseased group. After dissection, the serum electrolytes, antioxidant enzymes, and choline esterase enzymes were measured in different organs. The serum parameters creatinine, urea, and bilirubin increased after extract treatment. Similarly, the level of antioxidant enzymes like peroxidases (POD), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and thiobarbituric acid reactive substance (TBARS) in the extract-treated group showed improved results that were close to the normal control group. The enzyme (lipase, insulin, amylase, and acetylcholine) levels were found enhanced in extract groups as compared to diseased rats. High-performance liquid chromatography (HPLC) was used to determine the level of dopamine and serotonin neurotransmitters, which were increased significantly for P. dactylifera seeds with values of 0.18 μg/mg tissue and 0.56 μg/mg tissue, respectively. Overall, results showed that P. dactylifera seeds proved to be quite efficient in improving the memory and behavior of treated rats. The antioxidants and enzymes were also increased; therefore, it may be a potential candidate for treating AD.
    Type of Medium: Online Resource
    ISSN: 2314-6141 , 2314-6133
    URL: Article
    DOI: 10.1155/2023/1725638
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2698540-8
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  • 7
    Online Resource
    Online Resource
    Ethnoveterinary botanical survey of medicinal plants used in Pashto, Punjabi and Saraiki communities of Southwest Pakistan
    Wiley ; 2021
    In:  Veterinary Medicine and Science Vol. 7, No. 5 ( 2021-09), p. 2068-2085
    Details
    In: Veterinary Medicine and Science, Wiley, Vol. 7, No. 5 ( 2021-09), p. 2068-2085
    Abstract: Medicinal plants are highly used in the ethnoveterinary practice as considerable livestock resources in remote areas. The aim of the present study is to explore the ethnoveterinary medicinal practices in three different communities and discuss the cross‐cultural consensus on the usage of medicinal plants for the treatment of animals. The field survey was conducted by the animal healers of the area during the different seasons of plant growth. A total of 83 informants were interviewed through Semi‐structured interview involving experts of traditional knowledge in 21 localities of the three regions (Zhob, D. I. Khan and Mianwali) were conducted. Findings of the study were quantitatively analyzed through the informant consensus factors to identify the homogeneity information provided by the informants. Furthermore, cross‐culture consensuses were analyzed and recorded data were represented in a tabulated and Venn diagrams. In particularly, 59 species of plants were documented in the comparative analysis. Among them, 32 plant species were recorded in Pashto community, while Punjabi and Sarakai communities exhibited nine and four plant species, respectively. Whereas cross‐cultural analysis showed 14 medicinal plants that were commonly utilized by three different ethnic communities, that indicated low interregional consensus in regard to ethnoveterinary practices of medicinal plants. The current study showed that different communities and ethnic groups sharing some traditional knowledge and cross‐culturally approaches have been reported from traditional uses of plants against livestock's diseases. Therefore, current findings are the opportunities to scrutinize the plants for the discovery of new drug sources for humans and animals.
    Type of Medium: Online Resource
    ISSN: 2053-1095 , 2053-1095
    URL: Issue
    URL: Article
    DOI: 10.1002/vms3.v7.5
    DOI: 10.1002/vms3.582
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2819409-3
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  • 8
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    Online Resource
    Effects of Envenomation on Survival and Development of the Host-Parasitoid System of Galleria mellonella (Lepidoptera: Pyralidae) and Bracon hebetor (Hymenoptera: Braconidae)
    ResearchersLinks Ltd ; 2023
    In:  Pakistan Journal of Zoology Vol. 55, No. 3 ( 2023)
    Details
    In: Pakistan Journal of Zoology, ResearchersLinks Ltd, Vol. 55, No. 3 ( 2023)
    Type of Medium: Online Resource
    ISSN: 0030-9923
    URL: Article
    DOI: 10.17582/journal.pjz/20210728050722
    Language: Unknown
    Publisher: ResearchersLinks Ltd
    Publication Date: 2023
    detail.hit.zdb_id: 2626365-8
    SSG: 12
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  • 9
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    Online Resource
    Impact of the COVID-19 Pandemic on Cancer Patients: Challenges and Practical Approach to Management
    National University of Medical Sciences ; 2020
    In:  Life and Science Vol. 1, No. supplement ( 2020-12-23), p. 6-
    Details
    In: Life and Science, National University of Medical Sciences, Vol. 1, No. supplement ( 2020-12-23), p. 6-
    Abstract: The coronavirus 2019 (COVID-19) outbreak has rapidly spread worldwide, which poses great challenges to the healthcare system around the world. This pandemic has shown that globally medical community has no care- models to deal with the effects imposed on patients with chronic illnesses. Appropriate and timely diagnosis and treatment of this highly vulnerable immunocompromised population is mandatory. Prominent challenges faced during the current outbreak include resource allocation, management of patients suffering from and follow-up-phases, patient fear and protection of healthcare workers. During Pandemic, the major management strategies for cancer patients comprise education about personal protective measures, symptoms of COVID- 19, emotional support, clear communication about infection control measures and clinical care. To decrease the risk of exposure, active individualized cancer intervention is required with reduce outpatient visits and maximum telemedicine. Currently, international guidelines to manage cancer patients in any infectious pandemic are not available. The development of a complete contingency plan with guidelines for the safety and patients care will pose beneficial effects by minimizing the risks of morbidity and mortality.
    Type of Medium: Online Resource
    ISSN: 2708-2970 , 2521-0475
    URL: Article
    DOI: 10.37185/LnS.1.1.152
    Language: Unknown
    Publisher: National University of Medical Sciences
    Publication Date: 2020
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  • 10
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    Perspectives of emerging zoonoses – challenges and opportunities
    National University of Medical Sciences ; 2020
    In:  Life and Science Vol. 1, No. 4 ( 2020-10-16), p. 5-
    Details
    In: Life and Science, National University of Medical Sciences, Vol. 1, No. 4 ( 2020-10-16), p. 5-
    Abstract: Zoonoses are the infectious diseases that are linked with human and animals. During the last few decades, human-animal linkages and close associations have been increased the zoonotic diseases threats upto several times. The increasing demand and love for animal based edible proteins have resulted higher rate of human-animal interaction. Greater the interaction rate, higher the zoonotic disease threat. Moreover, the human, animal and environmental factors have increased the threat of vector-borne and food–borne illnesses globally. In the present study, we have focused on vector-borne and food-borne diseases that are transmissible between human and animal species. We have highlighted a few opportunities through which we can reduce the chances of emerging zoonotic disease illnesses in developing countries such as Pakistan.
    Type of Medium: Online Resource
    ISSN: 2708-2970 , 2521-0475
    URL: Article
    DOI: 10.37185/LnS.1.1.70
    Language: Unknown
    Publisher: National University of Medical Sciences
    Publication Date: 2020
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