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  • 1
    In: Molecules, MDPI AG, Vol. 21, No. 9 ( 2016-08-26), p. 1124-
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2016
    detail.hit.zdb_id: 2008644-1
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  • 2
    In: BASE, University of Liege
    Abstract: Efficacy of fungi Volatile Organic Compounds. A review Introduction. Excessive use of chemical fungicides continues to drive research towards environmentally friendly and innovative alternatives for crop protection. Literature. Fungi produce various mixtures of compounds in the gas phase, called Volatile Organic Compounds (VOCs). They are able to diffuse into the soil and into the atmosphere and inhibit the activities of fungal pathogens. In this section, we will summarize recent knowledge on the inhibitory potential of Volatile Organic Compounds against pathogenic fungi with a focus on the effect of fungal VOCs. In practice, we will unveil initial research revealing their mode of action and any non-specific phytotoxic effects on the environmental microbiome and on plants. Conclusions. This article discusses new techniques used by researchers that focus on mycofumigation to optimize the formulation of a new generation of biofungicides. Thus, a new horizon is emerging for biological control of crop diseases.
    Type of Medium: Online Resource
    ISSN: 1780-4507 , 1370-6233
    Language: French
    Publisher: University of Liege
    Publication Date: 2020
    detail.hit.zdb_id: 2045320-6
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  • 3
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-05-16)
    Abstract: Banana ( Musa sp .) ranks fourth in term of worldwide fruit production, and has economical and nutritional key values. The Cavendish cultivars correspond to more than 90% of the production of dessert banana while cooking cultivars are widely consumed locally around the banana belt production area. Many plants, if not all, produce Volatile Organic Compounds (VOCs) as a means of communication with their environment. Although flower and fruit VOCs have been studied for banana, the VOCs produced by the plant have never been identified despite their importance in plant health and development. A volatile collection methodology was optimized to improve the sensitivity and reproducibility of VOCs analysis from banana plants. We have identified 11 VOCs for the Cavendish, mainly (E,E)-α-farnesene (87.90 ± 11.28 ng/μl), methyl salicylate (33.82 ± 14.29) and 6-methyl-5-hepten-2-one (29.60 ± 11.66), and 14 VOCs for the Pacific Plantain cultivar, mainly (Z,E)-α-farnesene (799.64 ± 503.15), (E,E)-α-farnesene (571.24 ± 381.70) and (E) β ocimene (241.76 ± 158.49). This exploratory study paves the way for an in-depth characterisation of VOCs emitted by Musa plants.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2615211-3
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  • 4
    In: Plants, MDPI AG, Vol. 11, No. 2 ( 2022-01-15), p. 226-
    Abstract: The banana mild mosaic virus (BanMMV) (Betaflexiviridae, Quinvirinae, unassigned species) is a filamentous virus that infects Musa spp. and has a very wide geographical distribution. The current BanMMV indexing process for an accession requires the testing of no less than four plants cultivated in a greenhouse for at least 6 months and causes a significant delay for the distribution of the germplasm. We evaluated the sensitivity of different protocols for BanMMV detection from in vitro plants to accelerate the testing process. We first used corm tissues from 137 in vitro plants and obtained a diagnostic sensitivity (DSE) of only 61% when testing four plants per accession. After thermotherapy was carried out to eliminate BanMMV infection, the meristem was recovered and further grown in vitro. The same protocol was evaluated in parallel on the corm tissue surrounding the meristem, as a rapid screening to evaluate virus therapy success, and was compared to the results obtained following the standard protocol. The obtained results showed 28% false negatives when conducting testing from corm tissues, making this protocol unsuitable in routine processes. Furthermore, RT-PCR and high-throughput sequencing (HTS) tests were applied on tissues from the base (n = 39) and the leaves (n = 36). For RT-PCR, the average DSE per sample reached 65% from either the base or leaves. HTS was applied on 36 samples and yielded 100% diagnostic specificity (DSP) and 100% DSE, whatever the sampled tissue, allowing the identification of a new Betaflexiviridae species infecting Musa. These results suggest that a reliable diagnostic of BanMMV from in vitro plants using RT-PCR or HTS technologies might represent an efficient alternative for testing after greenhouse cultivation.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2704341-1
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  • 5
    Online Resource
    Online Resource
    Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) ; 2019
    In:  Spanish Journal of Agricultural Research Vol. 17, No. 2 ( 2019-07-26), p. e1006-
    In: Spanish Journal of Agricultural Research, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Vol. 17, No. 2 ( 2019-07-26), p. e1006-
    Abstract: In order to reduce the use of chemical pesticides, great interest has been focused on environment-friendly biological control agents and botanicals that preserve biodiversity. In this context, our study aimed to assess the antifungal and herbicidal activities of Rosmarinus officinalis essential oil (EO) to find an alternative to synthetic pesticides. The chemical composition of R. officinalis essential oil was determined by gaz chromatography-mass spectrometry analysis (GC-MS). Results showed that R. officinallis EO was rich in monoterpenes and the major constituents were 1,8-cineole (54.6%), camphor (12.27%) and α-pinene (7.09%). However, under laboratory conditions, two tests were carried out. The first one consisted on the study of EO antifungal activity using ELISA microplates and the second one consisted on evaluating the effect of EO on seedling growth of weeds. It was confirmed that this EO significantly inhibits spore germination of Fusarium oxysporum, Fusarium culmorum, Penicillium italicum and at 6 mM, the percentage of inhibition reached 100% on Fusarium oxysporum. Indeed, EO slows down seedling growth of Trifolium incarnatum, Silybum marianum, and Phalaris minor. In fact, EO at 5 mM completely inhibits seed germination. On the other hand, another experiment was carried out to evaluate the herbicidal activity by spraying EO on weeds. This showed that a novel herbicide formulation was set up for the first time to improve the activity of R. officinalis EO on post-emergence. Overall, R. officinalis EO can be suggested as a potential eco-friendly pesticide and suitable source of natural compounds potentially usable as natural pesticides.
    Type of Medium: Online Resource
    ISSN: 2171-9292
    Language: Unknown
    Publisher: Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA)
    Publication Date: 2019
    detail.hit.zdb_id: 2172833-1
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  • 6
    In: Sustainability, MDPI AG, Vol. 12, No. 16 ( 2020-08-05), p. 6313-
    Abstract: Anaerobic soil disinfestation (ASD), as a bio-fumigation technology, has been developed to control soil-borne pests. There is increasing evidence showing that carbon sources and cover tarps play an important role in the ASD suppression of soil-borne pests, but little is known about the effect of composted chicken manure (CCM) and totally impermeable films (TIF) against soil-borne pests in the strawberry production system. In experiments, the colonies of Fusarium spp. and Phytophthora spp., which are recognized to cause strawberry soil-borne diseases, decreased significantly after ASD. The soil promoted a significant increase in ammonium nitrogen, nitrate-nitrogen and organic matter, but a decrease in oxidation-reduction potential after ASD. Besides, the strawberry plant height, stem thickness and yield were significantly higher than in the non-amended soil. Compared to the untreated control, ASD, both at 6 and 12 ton/ha of CCM, significantly (p = 0.05) increased strawberry marketable yield and income. The economic benefit could be due to the suppression of soil-borne diseases and the improvement of soil nutrition. The soil bacterial and fungal diversity and richness increased after soil fumigation. The increased presence of biological control agents led to the suppression of soil-borne pathogens. In summary, ASD with CCM amendments could be applied in pre-plant fumigation to control strawberry soil-borne pests, strengthen soil fertility, improve crop yield and increase growers’ income.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2518383-7
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