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  • 1
    In: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, Mary Ann Liebert Inc, Vol. 37, No. 2 ( 2018-04), p. 78-86
    Type of Medium: Online Resource
    ISSN: 2167-9436
    Language: English
    Publisher: Mary Ann Liebert Inc
    Publication Date: 2018
    detail.hit.zdb_id: 2709045-0
    SSG: 15,3
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  • 2
    Online Resource
    Online Resource
    The Electrochemical Society ; 2019
    In:  ECS Meeting Abstracts Vol. MA2019-02, No. 44 ( 2019-09-01), p. 2061-2061
    In: ECS Meeting Abstracts, The Electrochemical Society, Vol. MA2019-02, No. 44 ( 2019-09-01), p. 2061-2061
    Abstract: The recycling of human urine has become a main research area in wastewater treatment due to the increased demand for water. Urine is composed of 95% water, 2% urea and other organic and inorganic compounds. Urea is a toxic molecule that may cause renal failure and gastrointestinal bleeding. Nevertheless, it is difficult to remove by common methods since it is a small, uncharged molecule. In this work, the use of Proteus vulgaris is proposed, a bacterium that contains urease, to catalyze the urea conversion to ammonia. The latter can then be oxidized by a platinum electrode while producing a current leaving a urea-ammonia-free solution. This system was first tested in synthetic human urine (SHU) simulating the ureolytic bacterium behavior. The ammonia oxidation current (AOC) was evaluated by cyclic voltammetry (CV) using Pt electrode in SHU, obtaining better results when less urea was present in solution. Preliminary, AOC was evaluated while the bacterial grew in Enriched Synthetic Human Urine (SHU + growth factors). The experiment was carried out for 24 hours showing not only bacterial viability and actual growth in the medium but an increased AOC over time.
    Type of Medium: Online Resource
    ISSN: 2151-2043
    Language: Unknown
    Publisher: The Electrochemical Society
    Publication Date: 2019
    detail.hit.zdb_id: 2438749-6
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  • 3
    Online Resource
    Online Resource
    The Electrochemical Society ; 2019
    In:  ECS Meeting Abstracts Vol. MA2019-04, No. 8 ( 2019-06-30), p. 404-404
    In: ECS Meeting Abstracts, The Electrochemical Society, Vol. MA2019-04, No. 8 ( 2019-06-30), p. 404-404
    Abstract: Current urine recycling treatments are unable to completely recover clean water free of toxic metabolic products such as urea. Urea is a small and uncharged molecule, diffulting its removal from wastewater. Previous publications from our research group reported the use of immobilized urease enzyme on carbon matrix as a urea bioreactor to convert urea to ammonia. The stability of the enzyme was limitation for long time use. For this, a microbial system has been selected. An ureolysis system in a microbial chemostat using robust Proteus vulgaris bacteria that can transform urea to ammonia is being proposed. The chemostat will be coupled to an ammonia fuel cell to further oxidize ammonia to nitrogen and generate energy. The first goal of this work is to establish the growth parameters for P. vulgaris in synthetic human urine (SHU) by testing different formulations of synthetic urine similar to real urine. Our preliminary results indicate that P. vulgaris achieve exponential growth in Enriched Synthetic Human Urine during a 12 h Growth Assay using Genesys UV-Vis Spectrophotometer, and the Colony-forming Units counting technique. Future bacterial growth experiments will be done with a more chemically consistent synthetic human urine with normal human urine components such as amino acids.
    Type of Medium: Online Resource
    ISSN: 2151-2043
    Language: Unknown
    Publisher: The Electrochemical Society
    Publication Date: 2019
    detail.hit.zdb_id: 2438749-6
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  • 4
    In: ECS Meeting Abstracts, The Electrochemical Society, Vol. MA2021-02, No. 59 ( 2021-10-19), p. 1764-1764
    Type of Medium: Online Resource
    ISSN: 2151-2043
    Language: Unknown
    Publisher: The Electrochemical Society
    Publication Date: 2021
    detail.hit.zdb_id: 2438749-6
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  • 5
    Online Resource
    Online Resource
    The Electrochemical Society ; 2019
    In:  ECS Meeting Abstracts Vol. MA2019-02, No. 57 ( 2019-09-01), p. 2446-2446
    In: ECS Meeting Abstracts, The Electrochemical Society, Vol. MA2019-02, No. 57 ( 2019-09-01), p. 2446-2446
    Abstract: In order to facilitate extended human space travel, solutions and innovations are required to enable habitation in microgravity spacecraft habitats such as International space station (ISS), with limited earth availability. The existing supply of water on the International Space Station (ISS) is managed by Environmental Control and Life Support System (ECLSS) which recovers and recycles water from human waste. The best estimates available show that this system works at efficiencies between 75% to 85% water recovery which requires relatively frequent refueling to sustain life upon the ISS. As human missions travel further into the solar system the availability of resources to resupply will be diminished due to availability and ease of resupply. Therefore, next-generation systems to recycle water are required to reduce waste and improve system efficiency. Upon a critical evaluation of the existing ECLSS system we find that the water recovery system has lifetime/durability limitations due to inefficiencies within existing process like vapor compression distillation (VCD), reverse and forward osmosis (RO/FO) filtration. Requiring the supply of complex hazardous chemicals to treat these system components such that they maintain their targeted performance. Within this context, Faraday Technology Inc. and the University of Puerto Rico (UPR) are developing a custom bio-electrochemical system. Within this system, a bioreactor will convert urea from the waste water to ammonia by hydrolysis: NH 2 (CO)NH 2 + H 2 O → 2NH 3 + CO 2 (1) Next the effluent of the bioreactor will flow through the ammonia oxidation reactor: 2NH 3 → N 2 + 3H 2 (2) Thus, generating urea free waste water effluent for further filtration and enhancement. The developed technology has the potential to be compatible with existing ECLSS systems and be an integral part of the closed loop living systems required for long term life support on NASA’s manned space missions. In this talk we will be discussing the ongoing developments at Faraday. Where we have developed a custom bench-scale ammonia reactor and confirmed that the reactor can oxidize ammonia from basic electrolytes. Further, the ammonia reactor was used to demonstrate the potential of oxidizing ammonia utilizing near neutral urine simulants. These results are promising and an early indication that the bio-electrochemical system will be able to operate with high performance efficiency. We are continuing the technology development efforts by (1) leveraging existing knowledge to design and test the bio-electrochemical reactor under zero gravity conditions; (2) exploring the efficacy of ( P. Vulgaris ) bacteria for urea bioreactor, (3) evaluating electrocatalyst for ammonia oxidation reactor, (4) optimizing the electrocatalytic efficiency and waste water treatment rate with urine simulants, (5) validating operation under zero gravity conditions; and (6) designing and building a demonstration-scale bio-electrochemical reactor unit capable of meeting NASA required specifications. Acknowledgements: The financial support of NASA Contract No. NNX17CA30P and 80NSSC18C0222 is acknowledged.
    Type of Medium: Online Resource
    ISSN: 2151-2043
    Language: Unknown
    Publisher: The Electrochemical Society
    Publication Date: 2019
    detail.hit.zdb_id: 2438749-6
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