In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 4 ( 2022-4-28), p. e1010465-
Abstract:
Although efficacious vaccines have significantly reduced the morbidity and mortality of COVID-19, there remains an unmet medical need for treatment options, which monoclonal antibodies (mAbs) can potentially fill. This unmet need is exacerbated by the emergence and spread of SARS-CoV-2 variants of concern (VOCs) that have shown some resistance to vaccine responses. Here we report the isolation of five neutralizing mAbs from an Indian convalescent donor, out of which two (THSC20.HVTR04 and THSC20.HVTR26) showed potent neutralization of SARS-CoV-2 VOCs at picomolar concentrations, including the Delta variant (B.1.617.2). One of these (THSC20.HVTR26) also retained activity against the Omicron variant. These two mAbs target non-overlapping epitopes on the receptor-binding domain (RBD) of the spike protein and prevent virus attachment to its host receptor, human angiotensin converting enzyme-2 (hACE2). Furthermore, the mAb cocktail demonstrated protection against the Delta variant at low antibody doses when passively administered in the K18 hACE2 transgenic mice model, highlighting their potential as a cocktail for prophylactic and therapeutic applications. Developing the capacity to rapidly discover and develop mAbs effective against highly transmissible pathogens like coronaviruses at a local level, especially in a low- and middle-income country (LMIC) such as India, will enable prompt responses to future pandemics as an important component of global pandemic preparedness.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010465
DOI:
10.1371/journal.ppat.1010465.g001
DOI:
10.1371/journal.ppat.1010465.g002
DOI:
10.1371/journal.ppat.1010465.g003
DOI:
10.1371/journal.ppat.1010465.g004
DOI:
10.1371/journal.ppat.1010465.g005
DOI:
10.1371/journal.ppat.1010465.g006
DOI:
10.1371/journal.ppat.1010465.g007
DOI:
10.1371/journal.ppat.1010465.s001
DOI:
10.1371/journal.ppat.1010465.s002
DOI:
10.1371/journal.ppat.1010465.s003
DOI:
10.1371/journal.ppat.1010465.s004
DOI:
10.1371/journal.ppat.1010465.s005
DOI:
10.1371/journal.ppat.1010465.s006
DOI:
10.1371/journal.ppat.1010465.s007
DOI:
10.1371/journal.ppat.1010465.s008
DOI:
10.1371/journal.ppat.1010465.s009
DOI:
10.1371/journal.ppat.1010465.s010
DOI:
10.1371/journal.ppat.1010465.s011
DOI:
10.1371/journal.ppat.1010465.s012
DOI:
10.1371/journal.ppat.1010465.s013
DOI:
10.1371/journal.ppat.1010465.s014
DOI:
10.1371/journal.ppat.1010465.s015
DOI:
10.1371/journal.ppat.1010465.s016
DOI:
10.1371/journal.ppat.1010465.s017
DOI:
10.1371/journal.ppat.1010465.s018
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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