In:
BMC Complementary and Alternative Medicine, Springer Science and Business Media LLC, Vol. 8, No. 1 ( 2008-12)
Abstract:
The present study was undertaken to evaluate the potential of the rhizomes of the Indian medicinal plant, Picrorhiza kurroa in healing indomethacin-induced acute stomach ulceration in mice and examine its capacity to modulate oxidative stress and the levels of prostaglandin (PGE 2 ) and EGF during the process. Methods Male swiss albino mice, ulcerated with indomethacin (18 mg/kg, p. o., single dose) were treated up to 7 days with different doses of the methanol extract of P. kurroa rhizomes (designated as PK). The healing capacity of the most effective dose of PK (20 mg/kg, p. o. × 3 d) was compared with that of omeprazole (Omez) (3 mg/kg, p. o. × 3 d). The effects of the drug-treatment for one and three days on the biochemical parameters were assessed by comparing the results with that of untreated mice of the 1 st and 3 rd day of ulceration. The stomach tissues of the mice were used for the biochemical analysis. Results The macroscopic indices revealed maximum ulceration on the 3 rd day after indomethacin administration, which was effectively healed by PK. Under the optimized treatment regime, PK and Omez reduced the ulcer indices by 45.1% ( P 〈 0.01), and 76.3% respectively ( P 〈 0.001), compared to the untreated ulcerated mice. Compared to the ulcerated untreated mice, those treated with PK for 3 days showed decreased the levels of thiobarbituric acid reactive substances (TBARS) (32.7%, P 〈 0.05) and protein carbonyl (37.7%, P 〈 0.001), and increased mucin (42.2%, P 〈 0.01), mucosal PGE 2 (21.4%, P 〈 0.05), and expressions of COX-1 and 2 (26.9% and 18.5%, P 〈 0.05), EGF (149.0%, P 〈 0.001) and VEGF (56.9%, P 〈 0.01). Omez reduced the TBARS (29.4%, P 〈 0.05), and protein carbonyl (38.9%, P 〈 0.001), and increased mucin (38.3%, P 〈 0.01), without altering the other parameters significantly. Conclusion PK (20 mg/kg, p. o. × 3 days) could effectively heal indomethacin-induced stomach ulceration in mice by reducing oxidative stress, and promoting mucin secretion, prostaglandin synthesis and augmenting expressions of cyclooxygenase enzymes and growth factors.
Type of Medium:
Online Resource
ISSN:
1472-6882
DOI:
10.1186/1472-6882-8-3
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2008
detail.hit.zdb_id:
2050429-9
detail.hit.zdb_id:
3037610-5
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