In:
Biology and Fertility of Soils, Springer Science and Business Media LLC, Vol. 59, No. 2 ( 2023-02), p. 217-232
Abstract:
Soil chemical properties and microbiome composition impact N 2 O emission potential, but the relative importance of these factors as determinants of N 2 O emission in denitrifying systems is rarely tested. In addition, previous linkages between microbiome composition and N 2 O emission potential rarely demonstrate causality. Here, we determined the relative impact of bacteriome composition (i.e., soil extracted bacterial cells) and soil water extract (i.e., water extractable chemicals and particles below 0.22 µm) on N 2 O emission potential utilizing an anoxic cell-based assay system. Cells and water extract for assays were sourced from soils with contrasting N 2 O/N 2 O + N 2 ratios, combined in various combinations and denitrification gas production was measured in response to nitrate addition. Analysis of 16S amplicon sequencing data revealed similarity in composition between extracted and parent soil bacteriomes. Average directionless effects of cell and water extract on N 2 O/N 2 O + N 2 (Cell: ∆0.17, soil water extract: ∆0.22) and total N 2 O hypothetically emitted (Cell: ∆2.62 µmol-N, soil water extract: ∆4.14 µmol-N) across two assays indicated water extract is the most important determinant of N 2 O emissions. Independent pH differences of just 0.6 points impacted N 2 O/N 2 O + N 2 on par with independent water extract differences, supporting the dominance of this variable in previous studies. However, impacts on overall N 2 O hypothetically emitted were smaller, suggesting that soil pH manipulation may not necessarily be a successful approach to mitigate emissions. In addition, we observed increased N 2 O accumulation and emission potential at the end of incubations concomitant with predicted decreases in carbon (C) availability, suggesting that C limitation increases N 2 O emission transiently with the magnitude of emission dependent on both chemical and bacteriome controls.
Type of Medium:
Online Resource
ISSN:
0178-2762
,
1432-0789
DOI:
10.1007/s00374-022-01690-5
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2023
detail.hit.zdb_id:
1473419-9
detail.hit.zdb_id:
742137-0
SSG:
12
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