In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 10 ( 2022-10-24), p. e0010890-
Abstract:
Aedes aegypti is the primary vector of dengue virus (DENV) and other arboviruses. Previous literature suggests that vertebrate and invertebrate lipids and the nutritional status of mosquitoes modify virus infection. Here, we developed a vertebrate lipid-depleted Ae . aegypti cell line to investigate if chronic depletion of vertebrate lipids normally present in a blood meal and insect cell culture medium would impact cell growth and virus infection. Chronic depletion of vertebrate lipids reduced cell size and proliferation, although cells retained equivalent total intracellular lipids per cell by reducing lipolysis and modifying gene expression related to sugar and lipid metabolism. Downregulation of innate immunity genes was also observed. We hypothesized that chronic depletion of vertebrate lipids would impact virus infection; however, the same amount of DENV was produced per cell. This study reveals how Ae . aegypti cells adapt in the absence of vertebrate lipids, and how DENV can replicate equally well in cells that contain predominately vertebrate or invertebrate lipids.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010890
DOI:
10.1371/journal.pntd.0010890.g001
DOI:
10.1371/journal.pntd.0010890.g002
DOI:
10.1371/journal.pntd.0010890.g003
DOI:
10.1371/journal.pntd.0010890.g004
DOI:
10.1371/journal.pntd.0010890.g005
DOI:
10.1371/journal.pntd.0010890.g006
DOI:
10.1371/journal.pntd.0010890.g007
DOI:
10.1371/journal.pntd.0010890.g008
DOI:
10.1371/journal.pntd.0010890.g009
DOI:
10.1371/journal.pntd.0010890.t001
DOI:
10.1371/journal.pntd.0010890.s001
DOI:
10.1371/journal.pntd.0010890.s002
DOI:
10.1371/journal.pntd.0010890.s003
DOI:
10.1371/journal.pntd.0010890.s004
DOI:
10.1371/journal.pntd.0010890.s005
DOI:
10.1371/journal.pntd.0010890.s006
DOI:
10.1371/journal.pntd.0010890.s007
DOI:
10.1371/journal.pntd.0010890.s008
DOI:
10.1371/journal.pntd.0010890.s009
DOI:
10.1371/journal.pntd.0010890.r001
DOI:
10.1371/journal.pntd.0010890.r002
DOI:
10.1371/journal.pntd.0010890.r003
DOI:
10.1371/journal.pntd.0010890.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5
Permalink