In:
American Journal of Physiology-Legacy Content, American Physiological Society, Vol. 207, No. 6 ( 1964-12-01), p. 1221-1225
Abstract:
Mammalian cells grown in tissue culture have been shown previously to take up considerable quantities of cholesterol from the growth medium. When cells grown on cholesterol-C 14 supplemented medium were transferred to unlabeled medium containing serum, excretion of cholesterol into the outside medium took place. When cell cholesterol was labeled by intracellular synthesis from mevalonate-C 14 precursor, it also was excreted readily into the serum medium. This excretion did not take place in serum-free medium and was found to be stimulated by a nondialyzable, thermolabile component of human serum. Horse, chicken, calf, and rabbit serum also showed stimulation ability. The process of cholesterol excretion appears to be of general occurrence. It was found in both strains of cultured cells examined (mouse fibroblasts and lymphoblasts) and also in strips of rabbit aorta incubated in vitro.
Type of Medium:
Online Resource
ISSN:
0002-9513
DOI:
10.1152/ajplegacy.1964.207.6.1221
Language:
English
Publisher:
American Physiological Society
Publication Date:
1964
detail.hit.zdb_id:
2948-8
SSG:
12
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