In:
Journal of Biomedical Materials Research Part A, Wiley, Vol. 107, No. 10 ( 2019-10), p. 2183-2194
Abstract:
Hypoxia and limited vascularization inhibit bone growth and recovery after surgical debridement to treat osteomyelitis. Similarly, despite significant efforts to create functional tissue‐engineered organs, clinical success is often hindered by insufficient oxygen diffusion and poor vascularization. To overcome these shortcomings, we previously used the oxygen carrier perfluorooctane (PFO) to develop PFO emulsion‐loaded hollow microparticles (PFO‐HPs). PFO‐HPs act as a local oxygen source that increase cell viability and maintains the osteogenic differentiation potency of human periosteum‐derived cells ( h PDCs) under hypoxic conditions. In the present study, we used a miniature pig model of mandibular osteomyelitis to investigate bone regeneration using h PDCs seeded on PFO‐HPs ( h PDCs/PFO‐HP) or h PDCs seeded on phosphate‐buffered saline (PBS)‐HPs ( h PDCs/PBS‐HP). Osteomyelitis is characterized by a series of microbial invasion, vascular disruption, bony necrosis, and sequestrum formation due to impaired host defense response. Sequential plain radiography, computed tomography (CT), and 3D reconstructed CT images revealed new bone formation was more advanced in defects that had been implanted with the h PDCs/PFO‐HPs than in defects implanted with the h PDCs/PBS‐HP. Thus, PFO‐HPs are a promising tissue engineering approach to repair challenging bone defects and regenerate structurally organized bone tissue with 3D architecture.
Type of Medium:
Online Resource
ISSN:
1549-3296
,
1552-4965
DOI:
10.1002/jbm.a.v107.10
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
1477192-5
SSG:
12
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