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  • 1
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    Online Resource
    PRODUKSI ENZIM SELULASE DARI BAKTERI TS2b YANG DIISOLASI DARI RUMPUT LAUT DAN PEMANFAATANNYA DALAM MENGHIDROLISIS KULIT UBI KAYU DAN DAUN UBI KAYU SEBAGAI BAHAN BAKU PAKAN IKAN Irma
    Agency for Marine and Fisheries Research and Development ; 2014
    In:  Jurnal Riset Akuakultur Vol. 9, No. 2 ( 2014-08-30), p. 263-
    Details
    In: Jurnal Riset Akuakultur, Agency for Marine and Fisheries Research and Development, Vol. 9, No. 2 ( 2014-08-30), p. 263-
    Abstract: Upaya untuk mendapatkan bahan baku pakan alternatif masih perlu dilakukanmengingat makin meningkatnya harga pakan ikan. Salah satu bahan yang berpotensi untuk dimanfaatkan adalah kulit ubi kayu (KUK) dan daun ubi kayu (DUK). Tingginya kadar serat kasar khususnya selulosa dalam bahan baku tersebut, menjadi kendala dalam upaya pemanfaatannya. Penggunaan enzim selulase dapat menjadi alternatif untuk menangani masalah tersebut. Kemampuan komplek enzim selulase dari bakteri selulolitik dalam mendegradasi selulosa sangat beragam. Tujuan penelitian ini adalah memproduksi dan memanfaatkan enzim selulase dari bakteri yang diisolasi dari rumput laut untuk menghidrolisis KUK, DUK, dan selulosa murni (Carboxymethyl Cellulose). Penelitian ini dilaksanakan dalam dua tahap yaitu: pertama adalah produksi optimum enzim selulase dari bakteri TS2b dengan waktu inkubasi 24, 48, 72, 78, dan 96 jam dan kedua adalah tahap untuk mengetahui kemampuan enzim selulase bakteri TS2b dalam menghidrolisis KUK, DUK, dan Carboxymethyl Cellulose (CMC) (in vitro). Parameter yang diamati adalah aktivitas enzim selulase berdasarkan modifikasi metode Miller dan kadar gula pereduksi (glukosa) berdasarkan metode DNS. Hasil penelitian menunjukkan bahwa waktu optimum untuk produksi enzim selulase terjadi pada jam ke-78 yaitu sebesar 0,0214 U/mL dengan kadar glukosa yang dilepaskan sebesar 0,0231 mg/L. Daya hidrolisis enzim selulase tertinggi diperoleh pada substrat KUK dengan aktivitas enzim selulase dan kadar gula pereduksi yang dilepaskan berturutturut sebesar 0,0179 U/mL dan 0,9701 mg/L; sedangkan daya hidrolisis terendah diperoleh pada substrat DUK dengan aktivitas selulase sebesar 0,0015 U/mL dan kadar gula pereduksi yang dilepaskan sebesar 0,0787 mg/L. Enzim selulase isolat TS2b mempunyai kemampuan menghidrolisis substrat KUK dengan baik, tapi kurang efektif untuk menghidrolisis CMC dan DUK.
    Type of Medium: Online Resource
    ISSN: 2502-6534 , 1907-6754
    URL: Article
    DOI: 10.15578/jra.9.2.2014.263-270
    Language: Unknown
    Publisher: Agency for Marine and Fisheries Research and Development
    Publication Date: 2014
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  • 2
    Online Resource
    Online Resource
    N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro
    Institut Pertanian Bogor ; 2021
    In:  Current Biochemistry Vol. 6, No. 2 ( 2021-04-15), p. 92-105
    Details
    In: Current Biochemistry, Institut Pertanian Bogor, Vol. 6, No. 2 ( 2021-04-15), p. 92-105
    Abstract: Green tea (Camellia sinensis) is one of tradisional plants that have potential as anticancer. Thesample used in this research was commercial green tea extract. The purpose of this study was to test theantiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract wasfractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and water fraction of greentea have Lethality Concentration 50 (LC50) value of more than 1000 ppm. Fractions of ethanol 70% and nhexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of phytochemicalscreening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screeningresults of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancercells MCM-B2 and normal cells Vero by tripan blue staining method. The highest MCM-B2 cell inhibitoryactivity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction,59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Verocells characterized by not inhibiting normal cell proliferation.
    Type of Medium: Online Resource
    ISSN: 2355-7931 , 2355-7877
    URL: Issue
    URL: Article
    DOI: 10.29244/cb.6.2
    DOI: 10.29244/cb.6.2.5
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2021
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  • 3
    Online Resource
    Online Resource
    Aktivitas Antibakteri Isolat Bakteri Endofit dari Tanaman Miana (〈i〉Coleus scutellariodes〈/i〉 [L.] Benth.) terhadap 〈i〉Staphylococcus aureus〈/i〉 dan 〈i〉Escherichia coli〈/i〉
    Institut Pertanian Bogor ; 2014
    In:  Current Biochemistry Vol. 1, No. 1 ( 2014-04-19), p. 45-50
    Details
    In: Current Biochemistry, Institut Pertanian Bogor, Vol. 1, No. 1 ( 2014-04-19), p. 45-50
    Abstract: Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobial compounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass, therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolate and test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. against two pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophytic bacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolates of endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus, whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.
    Type of Medium: Online Resource
    ISSN: 2355-7931 , 2355-7877
    URL: Issue
    URL: Article
    DOI: 10.29244/cb.1.1
    DOI: 10.29244/cb.1.1.45-50
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2014
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  • 4
    Online Resource
    Online Resource
    Isolation and Characterization of Endophytic Bacteria from Tembelekan (〈i〉Lantana camara〈/i〉 L.) as Antibacterial Compounds Producer
    Institut Pertanian Bogor ; 2015
    In:  Current Biochemistry Vol. 2, No. 2 ( 2015-08-03), p. 86-98
    Details
    In: Current Biochemistry, Institut Pertanian Bogor, Vol. 2, No. 2 ( 2015-08-03), p. 86-98
    Abstract: Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.
    Type of Medium: Online Resource
    ISSN: 2355-7931 , 2355-7877
    URL: Issue
    URL: Article
    DOI: 10.29244/cb.2.2
    DOI: 10.29244/cb.2.2.86-98
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2015
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  • 5
    Online Resource
    Online Resource
    Immobilization of 〈i〉Rhizopus oryzae〈/i〉 Lipase on Zeolit, CaCO〈sub〉3〈/sub〉, Silica Gel, and Cow Bone
    Institut Pertanian Bogor ; 2015
    In:  Current Biochemistry Vol. 2, No. 2 ( 2015-08-03), p. 63-72
    Details
    In: Current Biochemistry, Institut Pertanian Bogor, Vol. 2, No. 2 ( 2015-08-03), p. 63-72
    Abstract: Food production in Indonesia is constrained by the high cost of lipase that is still imported from abroad. To overcome this problem, research of food production has been conducted using crude extract of lipase produced by indigenous species of fungi Rhizopus oryzae. The R. oryzae is edible indicating that it is safe to be used in the production of food products. Enzymes have an ability to catalyze specific chemical reactions with high efficiency and low energy cost. Enzyme immobilization is a recovery techique that has been studied in several years, using supporting materials as a medium to help enzyme dissolutions to the substrate. Several supporting materials such as zeolit, CaCO3, silica gel, and cow bone were selected by its ability to adsorb lipase. CaCO3 shows enzyme loading rate respectively 99.46%, giving more lipase to adsorb than zeolit (90.69%), cow bone (91.56%), and silica gel (59.63%). In this research, condition factors, such as optimum pH, optimum temperature, and storage ability of the matrix were investigated. Free lipase reacts optimally at pH 7 and temperature 30°C. Identical result showed for lipase in cow bone. Lipase in CaCO3 reacts optimally at pH 8 and temperature 35°C. Lipase in zeolit and silica gel reacts optimally at pH 8 and temperature 30°C. Half life time estimation was one week in storage condition temperature at 4°C and each optimum pH.
    Type of Medium: Online Resource
    ISSN: 2355-7931 , 2355-7877
    URL: Issue
    URL: Article
    DOI: 10.29244/cb.2.2
    DOI: 10.29244/cb.2.2.63-72
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2015
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  • 6
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    Online Resource
    Analysis of Metabolite Compound Profiles of Miana Leaves Endophytic Bacteria (Coleus scutellariodes) using GC-MS
    Institut Pertanian Bogor ; 2022
    In:  Current Biochemistry Vol. 8, No. 2 ( 2022-02-26), p. 63-67
    Details
    In: Current Biochemistry, Institut Pertanian Bogor, Vol. 8, No. 2 ( 2022-02-26), p. 63-67
    Abstract: Exploration of bioactive compounds from endophytic bacteria as novel source of antibacterial need to be explored, as an alternative to substitute synthetic antibiotics. Based on previous studies, DM6 was a potential isolate that showed antibacterial activity. The isolate has been identified as Brevibacillus sp. based on 16S rRNA analysis. This study aimed to identify antibacterial compounds produced by DM6 isolate using GC-MS. The GC-MS results showed that the chloroform extract of DM6 isolate contained : 1-Octadecene (CAS).alpha.-Octadecene, Hexanedioic acid, dioctyl ester (CAS) Dioctyl adipate, Phenol, 3,5-bis(1,1-dimethylethyl)- (CAS) 3,5-Di-tert-butylphenol, and 1,4-diaza-2,5-dioxobicyclo[4.3.0]nonane. Adipic acid/hexanedioic acid and phenols are also known to contained in the leaves of Coleus scutellariodes. This indicated that endophytic bacteria were able to synthesize antibacterial compounds which similar with the host plant.
    Type of Medium: Online Resource
    ISSN: 2355-7931 , 2355-7877
    URL: Article
    DOI: 10.29244/cb.8.2.2
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2022
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  • 7
    Online Resource
    Online Resource
    Delignifikasi Batang Kayu Sengon oleh 〈i〉Trametes versicolor〈/i〉
    Institut Pertanian Bogor ; 2014
    In:  Current Biochemistry Vol. 1, No. 1 ( 2014-04-19), p. 1-10
    Details
    In: Current Biochemistry, Institut Pertanian Bogor, Vol. 1, No. 1 ( 2014-04-19), p. 1-10
    Abstract: Delignification is a lignin degradation, a preliminary process in industries that used cellulose containing substrates. Sengon logs are often used for the material in pulp industry because it has high levels of cellulose and low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. The methods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, the rate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemical assay of sengon logs before and after delignification. The results of this study showed that delignification by T.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor). The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by 37.31% within 20 days. Chemical assay performed on delignified sengon wood showed decreased level of ethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicellulose level.
    Type of Medium: Online Resource
    ISSN: 2355-7931 , 2355-7877
    URL: Issue
    URL: Article
    DOI: 10.29244/cb.1.1
    DOI: 10.29244/cb.1.1.1-10
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2014
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  • 8
    Online Resource
    Online Resource
    Potentially Antihyperglycemic from Biomass and Phycocyanin of Spirulina Fusiformis Voronikhin by in Vivo Test
    Elsevier BV ; 2015
    In:  Procedia Chemistry Vol. 14 ( 2015), p. 211-215
    Details
    In: Procedia Chemistry, Elsevier BV, Vol. 14 ( 2015), p. 211-215
    Type of Medium: Online Resource
    ISSN: 1876-6196
    URL: Article
    DOI: 10.1016/j.proche.2015.03.030
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    detail.hit.zdb_id: 2515983-5
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  • 9
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    Online Resource
    Toxicity Study of Antidiabetics Functional Drink of Piper crocatum and Cinnamomum burmannii
    Bogor Agricultural University ; 2012
    In:  HAYATI Journal of Biosciences Vol. 19, No. 1 ( 2012-03), p. 31-36
    Details
    In: HAYATI Journal of Biosciences, Bogor Agricultural University, Vol. 19, No. 1 ( 2012-03), p. 31-36
    Type of Medium: Online Resource
    ISSN: 1978-3019
    URL: Article
    DOI: 10.4308/hjb.19.1.31
    Language: English
    Publisher: Bogor Agricultural University
    Publication Date: 2012
    detail.hit.zdb_id: 2621600-0
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  • 10
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    Online Resource
    Neurotrophic Activity of the Carrageenophyte Kappaphycus alvarezii Cultivated at Different Depths and for Different Growth Periods in Various Areas of Indonesia
    Hindawi Limited ; 2018
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2018 ( 2018-10-18), p. 1-7
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2018 ( 2018-10-18), p. 1-7
    Abstract: The carrageenophyte Kappaphycus alvarezii (Rhodophyta) has neurotrophic activity in primary hippocampal neurons. This seaweed is abundant and easily cultivated in tropical coastal areas. To determine the best growth conditions for neurotrophic activity, thalli were grown at different depths and for different periods in various areas of Indonesia. Neurotrophic activity was measured based on the number of primary neurites, the total length of the primary neurites, and the length of the longest neurite. K . alvarezii had higher neurotrophic activity than carrageenophytes K . striatum and Eucheuma denticulatum cultured under the same conditions. K . alvarezii grown at the surface for 45 days had higher (1.4- to 1.8-fold) neurotrophic activity than thalli grown at depth (2 m) or harvested sooner (15 days) ( P 〈 0.05). Relatively high activities were detected in thalli cultured at Ternate and Garut, Indonesia. Therefore, from a commercial perspective, the culture conditions at the surface for 45 days were optimal for the production of both neurotrophic compounds and carrageenan. K . alvarezii produced neurotrophic compounds under various environmental conditions, although some conditions were optimal.
    Type of Medium: Online Resource
    ISSN: 1741-427X , 1741-4288
    URL: Article
    DOI: 10.1155/2018/1098076
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2018
    detail.hit.zdb_id: 2148302-4
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