In:
Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 61, No. 11 ( 2010-01-08), p. 1511-1520
Abstract:
Rubus sanctus Schreb., known from the Bible as ‘holy thorn bush’, grows wild in Egypt. Rubus sanctus aqueous alcoholic extract (RE) contains a complicated phenolic mixture (ellagitanins, flavonoids and caffeic acid derivatives). In this study, the phytochemical investigation of the plant was re-evaluated. Herein, we report on the isolation and identification of three galloylated flavonoids, namely kaempferol-3-O-(6″-O-galloyl)-4C1-β-d-galactopyranoside, quercetin-3-O-(6″-O-galloyl)-4C1-β-d-galactopyranoside and myricetin-3-O-(6″-O-galloyl)- C1-β-d-galactopyranoside for the first time from the Rubus genus. We further aimed at evaluating the potential protective effects of RE against carbon tetrachloride (CCl4)-induced toxicity in isolated rat hepatocytes. Methods Based on an initial concentration-response experiment, a concentration of 100 μg/ml was selected to investigate the hepatoprotective activity of RE. Key findings Pretreatment with RE afforded protection as indicated by counteracting CCl4-induced cell death, and reduced glutathione depletion. In addition, RE ameliorated CCl4-induced enzyme leakage by 40% for lactate dehydrogenase, 30% for alanine aminotransferase and 20% for aspartate aminotransferase as compared with CCl4-treated cells. Moreover, RE counteracted CCl4-induced lipid peroxidation and inhibited spontaneous lipid peroxidation in the control group. Conclusions In conclusion, RE protects against CCl4-induced toxicity in isolated rat hepatocytes.
Type of Medium:
Online Resource
ISSN:
0022-3573
,
2042-7158
DOI:
10.1211/jpp.61.11.0011
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2010
detail.hit.zdb_id:
2041988-0
detail.hit.zdb_id:
2050532-2
SSG:
15,3
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