In:
Uludağ Üniversitesi Tıp Fakültesi Dergisi, Uludag Universitesi Tip Fakultesi Dergisi, Vol. 46, No. 2 ( 2020-08-01), p. 181-188
Abstract:
Kisspeptin neurons localized in the rostral periventricular area of the third ventricle are considered to provide a major stimulatory input to the GnRH neuronal network which is responsible for triggering the preovulatory LH surge. Noradrenaline (NA) is one of the main modulators of GnRH release. Although NA fibers are found in close opposition to kisspeptin neurons, interaction between kisspeptin and NA neurons needs to be clarify. Thus, present study was undertaken to determine whether kisspeptin can directly regulate NA release from brain slices prepared from medial preoptic area (MPOA) the rats. Brain slices prepared from female prepubertal, adolescents or adult Sprague Dawley rats were placed incubation wells containing oxygenated Krebs. After preincubation period, slices were incubated with two concentrations of kisspeptin (40 and 400 μM) for 60 min. At the end of kisspeptin incubation, incubation medium was used for determination of NA released from the slices. When used Ca+2-free medium, 400 μM of BAPTA was also added into the medium to chelate the extracellular Ca+2. Incubation of the brain slices prepared from MPOA of the adolescent and adult female rats with 40 and 400 μM of kisspeptin caused significant increase in NA release, unlike prepubertal rat. Since removal of the Ca+2 from the medium caused significant decline in kisspeptin-induced NA release (p‹0.05), a vesicular release mechanism seems to be likely involved. The results presented here probably indicate that in addition to direct effect of kisspeptin, it may also modulate GnRH release indirectly by increasing NA release from MPOA of hypothalamus.
Type of Medium:
Online Resource
ISSN:
1300-414X
DOI:
10.32708/uutfd.722097
Language:
Unknown
Publisher:
Uludag Universitesi Tip Fakultesi Dergisi
Publication Date:
2020
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