In:
Transfusion, Wiley, Vol. 42, No. 8 ( 2002-08), p. 999-1004
Abstract:
BACKGROUND : A size‐exclusion filter (Viresolve 180, Millipore Corp.) was tested for its ability to remove transmissible spongiform encephalopathies prion protein from an immune globulin preparation during ultrafiltration. STUDY DESIGN AND METHODS : Hamster‐adapted 263K scrapie brain homogenate (SBH) was spiked into Rh o (D) immune globulin (human) at 1 in 300 and 1 in 1000 dilutions. Before spiking, the SBH was treated with detergent, sonicated, and filtered through serial 0.45‐, 0.22‐, and 0.1‐μm filters to present a rigorous filter challenge. Process variables were monitored throughout the ultrafiltration to ensure that the spiked material did not compromise the membrane flux. Removal of scrapie prion protein (PrP Sc ) material was determined by use of a sensitive Western blot assay. RESULTS : The turbid SBH became completely translucent after sonication and passage through the 0.45‐, 0.22‐, and 0.1‐μm filters. The filtration of the immune globulin containing PrP Sc material was more difficult to perform than was filtration of immune globulin spiked with the normal cellular isoform. Even during tangential flow filtration, the fibril material prevented the PrP Sc ‐spiked immune globulin from passing as readily through the filter. Western blot results indicated a removal of greater than or equal to 2.5 log PrP Sc , while remaining within the normal filtration limits. CONCLUSIONS : The composition, physical condition, and the amount of SBH introduced have significant effects on the filtration of the immune globulin and the log removal values obtained. By use of a detergent‐treated, sonicated, and filtered preparation of SBH, it was demonstrated that the Viresolve 180 effectively removes PrP Sc from the immune globulin.
Type of Medium:
Online Resource
ISSN:
0041-1132
,
1537-2995
DOI:
10.1046/j.1537-2995.2002.00160.x
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
2018415-3
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