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  • 1
    Online Resource
    Online Resource
    UNS Solo ; 2018
    In:  Biodiversitas Journal of Biological Diversity Vol. 19, No. 3 ( 2018-05-01), p. 1110-1116
    In: Biodiversitas Journal of Biological Diversity, UNS Solo, Vol. 19, No. 3 ( 2018-05-01), p. 1110-1116
    Abstract: Darmawati IAP, Rai IN, Dwiyani R, Astarini IA. 2018. The diversity of wild Dendrobium (Orchidaceae) in Central Bali, Indonesia. Biodiversitas 19: 1110-1116. Dendrobium is the largest orchid genus of the Orchidaceae family. The research aimed to explore the species diversity of Dendrobium, dominance, and species similarity in some forest of Bali. The explored locations were grouped to lowland about 0-700 m asl. (Tejakula, Bukit Silangjana, Sepang Kelod, Mekori, and Busungbiu), transition land about 700-1100 m asl. (Sepang Kaje and Jatiluwih) and lower montane 1100-1500 m asl. (Natural Reserves of Lake Buyan-Tamblingan, Puncak Landep, and Lemukih). There were 24 species of Dendrobium in Central Bali, 2 spesies of which were not identified. The dominant species were D. acuminatissimum, D. crumenatum and D. linearifolium. The highest species diversity index of wild Dendrobium was present in lowland (16 species, 66.66%) followed by those in transition land and lower montane (12 species, 50.00% and 6 species, 20.83%, respectively). The pattern is also similar either for the species prosperity (3.875; 3.094, and 0.699, respectively). Uniformity of wild Dendrobium on transition land was 0.922, followed by those in lowland and lower montane (0.864 and 0.794). There were 6 similar species of Dendrobium found in lowlands and lower montane (42.857% equality index) and 2 similar species in lowlands and lower montane (19.047% equality index) and also 2 similar species in transition land and lower montane (23.359% equality index). D. macrophylum, D. heterocarpum and D.secundum, their presence are pronely threatened with extinction so conservation is necessary.
    Type of Medium: Online Resource
    ISSN: 2085-4722 , 1412-033X
    Language: Unknown
    Publisher: UNS Solo
    Publication Date: 2018
    detail.hit.zdb_id: 2660049-3
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  • 2
    Online Resource
    Online Resource
    Universitas Udayana ; 2018
    In:  International Journal of Biosciences and Biotechnology Vol. 5, No. 2 ( 2018-05-21), p. 111-
    In: International Journal of Biosciences and Biotechnology, Universitas Udayana, Vol. 5, No. 2 ( 2018-05-21), p. 111-
    Abstract: From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3rd and 5th clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.
    Type of Medium: Online Resource
    ISSN: 2655-9994 , 2303-3371
    URL: Issue
    Language: Unknown
    Publisher: Universitas Udayana
    Publication Date: 2018
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  • 3
    In: Biodiversitas Journal of Biological Diversity, UNS Solo, Vol. 22, No. 5 ( 2021-04-23)
    Abstract: Abstract. Darmawati IAP, Astarini IA, Yuswanti H, Fitriani Y. 2021. Conservation of Dendrobium spp. Bali orchids (Indonesia) through in vitro seed culture. Biodiversitas 22: 2554-2559. Orchid species, including Dendrobium spp., originated from Bali are threatened to extinction due to habitat destruction, illegal hunting, small population size, and the effects of global environmental change. Conservation strategies are therefore required to preserve the remaining germplasm, one of which is through in vitro seed propagation. This research aimed to investigate the pollination compatibility (i.e., pollination that produce fruits) of Dendrobium orchids originated from Bali, and to determine the effects of adding organic matters (i.e., coconut water, tomato extract, and peptone) to the base media on orchid seed germination under in vitro culture condition. Self-pollination and sibling pollination were implemented to seven orchid species, namely Dendrobium macrophyllum, D. heterocarpum, D. fimbriatum, D. linearifolium, D. spathilingue, D. secundum, and D. plicatile. The results showed that only four species, i.e., D. macrophyllum, D. heterocarpum, D. fimbriatum, and D. linearifolium, that produced fruits after being pollinated. The subsequent in vitro propagation of seeds produced by the four species showed that the addition of organic matters of tomato extract, coconut water, and peptone significantly affected the germination of Dendrobium forma Bali orchid seeds, indicated by different germination rates. The addition of 20% coconut water to Vacin and Went (VW) media gave the highest percentages of seed germination and protocorm formation for D. macrophyllum, D. heterocarpum, and D. fimbriatum at 98.33% and 95.00%; 91.67% and 91.67%; and 95% and 98.33%, respectively. Our findings can serve as baseline information when developing conservation strategies of Dendrobium orchids from Bali, particularly from the aspect of propagation
    Type of Medium: Online Resource
    ISSN: 2085-4722 , 1412-033X
    Language: Unknown
    Publisher: UNS Solo
    Publication Date: 2021
    detail.hit.zdb_id: 2660049-3
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  • 4
    Online Resource
    Online Resource
    Universitas Udayana ; 2017
    In:  Metamorfosa: Journal of Biological Sciences Vol. 4, No. 2 ( 2017-10-16), p. 271-
    In: Metamorfosa: Journal of Biological Sciences, Universitas Udayana, Vol. 4, No. 2 ( 2017-10-16), p. 271-
    Abstract: Anggrek Dendrobium sp. merupakan anggrek yang paling populer untuk tanaman hias pot dan bunga potong. Permintaan dari dalam negeri masih belum terpenuhi, dan masih mengimpor dari negeri tetangga. Salah satu usaha untuk memenuhi permintaan anggrek dalam dan luar negeri yaitu dengan perbanyakan vegetatif dengan menggunakan batang anggrek.  Penelitian ini bertujuan untuk mengetahui pengaruh ukuran batang dan konsentrasi zat pengatur tumbuh Zeatin terhadap pertumbuhan vegetatif anggrek Dendrobium sonia. Penelitian dilaksanakan pada November 2015 hingga Maret 2016 di shade house Puri Candra Asri, Batubulan, Gianyar – Bali. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan dua faktor yaitu konsentrasi Zeatin (0 mg/L, 0,1 mg/L dan 0,2 mg/L) dan panjang batang (0-10 cm, 10-20 cm dan 20-30 cm. Total ada sembilan kombinasi perlakuan dan masing – masing kombinasi perlakuan terdiri dari 7 ulangan.  Data kuantitatif berupa jumlah akar, panjang akar, jumlah tunas, panjang tunas dan jumlah daun pada 16 minggu setelah tanam (MST).  Data jumlah akar, panjang akar, panjang tunas dan jumlah daun dianalisis dengan Analysis of Variance (ANOVA) kemudian ilanjutkan dengan uji Duncan’s multiple range test (DMRT) pada taraf 5%. Pertumbuhan panjang akar dan jumlah daun terbaik yaitu pada perlakuan Z2B2 (Zeatin 0,1 mg/L + batang 10-20 cm), sedangkan pertumbuhan jumlah akar, jumlah tunas dan panjang tunas terbaik yaitu pada perlakuan Z2B3 (Zeatin 0,1 mg/L + batang 20-30 cm).
    Type of Medium: Online Resource
    ISSN: 2302-5697
    URL: Issue
    Language: Unknown
    Publisher: Universitas Udayana
    Publication Date: 2017
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  • 5
    In: Biodiversitas Journal of Biological Diversity, UNS Solo, Vol. 22, No. 4 ( 2021-03-11)
    Abstract: Abstract. Astarini IA, Ningsih EY, Simanungkalit D, Ardiana SA, Al Malik MD, Yusmalinda NLA, Sembiring A, Pertiwi NPD, Cahyani NKD, Collins A. 2021. Genetic variation of longtail tuna Thunnus tonggol landed in four fish markets in Indonesia based on mitochondrial DNA. Biodiversitas 22: 1644-1651. Longtail tuna (Thunnus tonggol, Family: Scombridae) is an economically valuable neritic species found in tropical and subtropical waters in the Indo-Pacific region. High catch numbers, which have been decreasing, could negatively impact this tuna’s population level. Little research has been conducted on the longtail tuna population in Indonesia. This study aims to determine the genetic diversity and potential population structure of longtail tuna landed in four fish markets in Indonesia (representing three sampling locations because two markets are relatively close to each other) based on sequences of a region of mitochondrial control region (d-loop). A total of 101 samples, out of 110, were identified and confirmed as T. tonggol species by amplifying and sequencing a fragment of d-loop (amplicons ranging from 482 - 523 bp). Neighbor-joining analysis resulted in a topology with all samples grouped into one clade with an average genetic distance of 0.020. Meanwhile, haplotype diversity (Hd) and nucleotide diversity (?) values of the longtail tuna samples were 0.9939 and 0.0192, respectively. The fixation index (Fst) value was -0.00507, with p 〉 0.05, which indicates that there is no significant population structure among the longtail tuna collected from four fish markets representing three sampling locations. The results of this analysis can be used as basic data in planning sustainable fisheries management efforts.
    Type of Medium: Online Resource
    ISSN: 2085-4722 , 1412-033X
    Language: Unknown
    Publisher: UNS Solo
    Publication Date: 2021
    detail.hit.zdb_id: 2660049-3
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  • 6
    Online Resource
    Online Resource
    Universitas Udayana ; 2020
    In:  Metamorfosa: Journal of Biological Sciences Vol. 6, No. 2 ( 2020-01-27), p. 277-
    In: Metamorfosa: Journal of Biological Sciences, Universitas Udayana, Vol. 6, No. 2 ( 2020-01-27), p. 277-
    Abstract: Penelitian bertujuan mengetahui karakteristik morfologi, jumlah padi varietas lokal, hubungan kekerabatan, jenis hama dan gulma yang mengganggu siklus hidup padi varietas lokal di Desa Wongaya Gede, Kecamatan Penebel, Kabupaten Tabanan, Bali. Penelitian dilakukan di tiga subak (Piak, Keloncing dan Bedugul) yang terdapat di Desa Wongaya Gede dan di Laboratorium Struktur dan Perkembangan Tumbuhan, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana pada bulan Agustus 2015 hingga Januari 2016. Penelitian di lapangan meliputi pengamatan karakteristik morfologi tanaman padi varietas lokal dari fase pembibitan hingga panen (85 karakter), inventarisasi hama dan gulma serta faktor lingkungan yang mempengaruhi pertumbuhan tanaman padi varietas lokal. Penelitian di laboratorium yaitu uji viabilitas serbuk sari tanaman padi varietas lokal dengan uji warna 1% aniline blue dalam laktofenol. Hasil penelitian menunjukkan karakteristik padi varietas lokal di Desa Wongaya Gede dapat dilihat pada ciri morfologi batang, daun, bunga, gabah, beras, agronomi dan ciri anatomi, tipe bentuk dan viabilitas serbuk sari serta tipe endosperma. Terdapat eanam padi varietas lokal dan satu padi varietas unggul di Desa Wongaya Gede yang terbagi menjadi dua golongan yaitu golongan Indica (Mansur dan Ketan Beton) dan Javanica (Merah Cendana, Injin, Putih Cempaka, Ketan Tahun dan Jaka Selem).  Gulma yang mengganggu siklus hidup padi varietas lokal di Desa Wongaya Gede terbagi menjadi dua golongan yaitu golongan teki (sedges) dan golongan berdaun lebar (broad leaves). Hama yang mengganggu siklus hidup padi varietas lokal di Desa Wongaya Gede terbagi menjadi tiga golongan yaitu golongan mamalia (hewan menyusui), golongan aves (burung) dan golongan insecta (serangga).
    Type of Medium: Online Resource
    ISSN: 2302-5697
    URL: Issue
    Language: Unknown
    Publisher: Universitas Udayana
    Publication Date: 2020
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  • 7
    Online Resource
    Online Resource
    Universitas Udayana ; 2020
    In:  Metamorfosa: Journal of Biological Sciences Vol. 7, No. 2 ( 2020-09-28), p. 71-
    In: Metamorfosa: Journal of Biological Sciences, Universitas Udayana, Vol. 7, No. 2 ( 2020-09-28), p. 71-
    Abstract: Pemuliaan tanaman adalah upaya pengembangan tanaman dengan memanipulasi sistem genetik tanaman untuk mendapatkan kultivar baru yang lebih unggul. Upaya pemuliaan tanaman seringkali menghasilkan biji yang bersifat inkompatibel, sehingga perlu dilakukan proses penyelamatan untuk mengurangi risiko gugurnya embrio. Penambahan asam giberelat merupakan salah satu cara untuk mempercepat perkecambahan biji yang tidak kompatibel. Tujuan penelitian adalah untuk menentukan pemberian konsentrasi asam giberelat yang tepat terhadap perkecambahan embrio globular jeruk hasil fusi protoplas. Penelitian dilakukan di Laboratorium Kultur In Vitro Kelompok Peneliti Biologi Sel dan Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian (BB BIOGEN), Badan Penelitian dan Pengembangan Pertanian, Kementrian Pertanian, Bogor. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan konsentrasi asam giberelat yaitu 0, 1, 2, 3 ppm, masing-masing terdiri dari 7 ulangan.  Eksplan yang digunakan adalah embrio globular jeruk galur FS 29 dan FS 89 hasil fusi protoplas jeruk Siam Simadu dengan Mandarin Satsuma berukuran rata-rata 3 mm. Pengamatan dilakukan terhadap persentase embrio yang berkecambah, waktu berkecambah, jumlah dan persentase kecambah sempurna dan tidak sempurna (abnormal, hanya bagian akar atau bagian tunas yang berkembang). Data dianalisis menggunakan uji Crosstab dengan software IBM SPSS Statistics 22.  Hasil penelitian menunjukkan penambahan asam giberelat pada media dapat mempercepat perkecambahan dan meningkatkan persentase kecambah yang dihasilkan. Media perkecambahan terbaik pada jeruk FS 29 adalah VMW + 2 ppm dan 3 ppm asam giberelat dengan persentase perkecambahan 85,7% dalam waktu 7 hari sedangkan media perkecambahan terbaik pada jeruk FS 89 adalah VMW + 1 ppm asam giberelat dengan persentase perkecambahan 100% dalam waktu 8,5 hari. Media VMW + 1, 2, 3 ppm asam giberelat pada jeruk FS29 dan VMW + 2 ppm dan 3 ppm asam giberelat pada jeruk FS89 baik digunakan untuk mendapatkan kecambah sempurna dengan tingkat keberhasilan sebesar 100%.   Kata kunci: Citrus sp., asam giberelat, inkompatibel, in vitro, perkecambahan embrio
    Type of Medium: Online Resource
    ISSN: 2302-5697
    URL: Issue
    Language: Unknown
    Publisher: Universitas Udayana
    Publication Date: 2020
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  • 8
    In: Musamus Fisheries and Marine Journal, Universitas Musamus Merauke, ( 2021-03-01), p. 107-115
    Abstract: Indonesia is the biggest tuna exporter in Southeast Asia. With a high number of tuna catch, it is worried that the catch will decrease tuna population, specifically longtail tuna. To anticipate the decrease, there needs to be a conservation program to protect longtail tunas from scarcity. One method used to protect longtail tuna is by genetic conservation. The aim of this research is to understand the genetic and phylogenetic variety of the longtail tuna in Pabean Surabaya Fish Market. The polymerase chain reaction was used to amplify segment of the mitochondrial control region gene from members of these sample, used forward primer CRK 5’-AGCTC AGCGC CAGAG CGCCG GTCTT GTAAA-3’ and reverse primer CRE 5’-CCTGA AGTAG GAACC AGATG-3’. Based on the sequencing process, 28 out of 29 samples longtail tuna  were analyzed successfully. The results of the 28 sample analysis of longtail tuna based on its genetic variety and phylogenetic tree reconstruction showed a haplotype variety (Hd) score of 1,00000, and nucleotide (π) variety of 0,1939. Genetic variety value showed that longtail tuna has great adaption capabilities toward environmental changes time to time. Phylogenetic tree reconstruction results showed 7 clades with a genetic range of 0,005 – 0,035, which shows that all samples are closely related. The results of this study can be used as basic information in forming regulations on longtail tuna sustainable management and genetic conservation.
    Type of Medium: Online Resource
    ISSN: 2656-7008 , 2654-9905
    URL: Issue
    Language: Unknown
    Publisher: Universitas Musamus Merauke
    Publication Date: 2021
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  • 9
    Online Resource
    Online Resource
    Institut Pertanian Bogor ; 2021
    In:  Jurnal Hortikultura Indonesia Vol. 12, No. 1 ( 2021-08-01), p. 51-58
    In: Jurnal Hortikultura Indonesia, Institut Pertanian Bogor, Vol. 12, No. 1 ( 2021-08-01), p. 51-58
    Abstract: Kentang (Solanum tuberosum L.) adalah salah satu komoditi pangan penting di dunia. Penyediaan benih kentang generasi awal yang bebas penyakit sistemik virus merupakan masalah utama dalam pembudidayaan kentang. Produksi benih kentang generasi awal berupa umbi mini di rumah kaca masih memiliki resiko kontaminasi oleh penyakit virus. Teknik produksi umbi mikro secara in vitro dapat menjadi metode alternatif untuk produksi benih sumber. Tujuan penelitian ini adalah untuk menentukan media yang optimum dalam produksi umbi mikro pada empat genotipe kentang, yaitu AOTX98202-1RU, ATX9202-3RU, ATTX98468-5R/Y dan ATTX98518-5P/Y. Keempat genotipe dikulturkan pada media Murashige and Skoog (MS) dengan tambahan 6% sukrosa, dengan atau tanpa 2 g L-1 phytagel, dan dengan atau tanpa 10 mg L-1 kinetin. Stek dua buku dikulturkan pada tiap botol kultur, masing – masing dengan lima ulangan. Kultur diinkubasi selama dua minggu pada suhu 23°C dengan fotoperiode 16 jam terang/hari, lalu suhu 16 °C selama 2 minggu dengan fotoperiode 16 jam terang/hari, dan suhu 16 °C dalam ruang gelap selama 6 minggu, dan diamati selama 10 minggu. Hasil penelitian menunjukkan bahwa tiap genotipe memiliki respon yang berbeda terhadap media. Perlakuan 2 g L-1 phytagel + 10 mg L-1 kinetin (T4) merupakan media yang efektif untuk mendorong pertumbuhan umbi mikro pada genotipe ATTX98468-5R/Y dan ATTX98518-5P/Y. Genotipe ATTX98468-5R/Y pada media T4 menghasilkan umbi mikro terbanyak (4.2 umbi mikro/botol), sedangkan ATTX98518-5P/Y menghasilkan rata-rata berat umbi tertinggi (363.6 mg). Hasil penelitian menunjukkan pentingnya untuk mengembangkan protokol khusus untuk tiap genotipe agar mendapatkan produksi umbi mikro yang optimal.
    Type of Medium: Online Resource
    ISSN: 2614-2872 , 2087-4855
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2021
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  • 10
    Online Resource
    Online Resource
    Science Press ; 2022
    In:  Journal of Hunan University Natural Sciences Vol. 49, No. 12 ( 2022-12-30), p. 151-158
    In: Journal of Hunan University Natural Sciences, Science Press, Vol. 49, No. 12 ( 2022-12-30), p. 151-158
    Abstract: Research has been carried out on the presence of wood-borer species in the mangrove forest of Nusa Lembongan, in May-June 2022. The study was conducted at five representative sites of mangrove forests in Nusa Lembongan. The data collection of the mangrove wood-borer was carried out by purposive sampling, which directly searches for dead mangrove stems, twigs, or roots that were attacked by the marine wood-borer found at each site. A total of There were new record four species of mangrove wood-borer, namely, Phaenops sp. beetle group (Coleoptera) and Cenoloba sp. moth group (Lepidoptera), two species of Mollusks, namely, Bankia minima and Bactronophorus sp. were identified. The larval stages of Phaenops sp. were found within the dead mangrove stems of Rhizophora apiculata and Sonneratia alba. Cenoloba sp. which was found in the larvae phase, the host plant was the dead stems of the mangrove Sonneratia alba. Bankia minima, the host plants are dead stems of mangroves Sonneratia alba, Rhizophora apiculata, Rhizophora stylosa and Avicennia sp. Bactronophorus sp., the host plant is dead wood from the mangrove Sonneratia alba, Rhizophora apiculata. The most widely distributed wood-borer species in the mangroves of Nusa Lembongan is Bankia minima. The results showed that four species of marine wood-borer are new records in the mangrove forest of Nusa Lembongan.
    Type of Medium: Online Resource
    ISSN: 1674-2974
    URL: Issue
    URL: Issue
    Language: English
    Publisher: Science Press
    Publication Date: 2022
    SSG: 11
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