In:
Frontiers in Bioengineering and Biotechnology, Frontiers Media SA, Vol. 11 ( 2023-9-18)
Abstract:
Haptophytes synthesize unique β-glucans containing more β-1,6-linkages than β-1,3 linkages, as a storage polysaccharide. To understand the mechanism of the synthesis, we investigated the roles of Kre6 (yeast 1,6-β-transglycosylase) homologs, PhTGS, in the haptophyte Pleurochrysis haptonemofera . RNAi of PhTGS repressed β-glucan accumulation and simultaneously induced lipid production, suggesting that PhTGS is involved in β-glucan synthesis and that the knockdown leads to the alteration of the carbon metabolic flow. PhTGS was expressed more in light, where β-glucan was actively produced by photosynthesis, than in the dark. The crude extract of E . coli expressing PhKre6 demonstrated its activity to incorporate 14 C-UDP-glucose into β-glucan of P. haptonemofera . These findings suggest that PhTGS functions in storage β-glucan synthesis specifically in light, probably by producing the β-1,6-branch.
Type of Medium:
Online Resource
ISSN:
2296-4185
DOI:
10.3389/fbioe.2023.1259587
DOI:
10.3389/fbioe.2023.1259587.s001
DOI:
10.3389/fbioe.2023.1259587.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2719493-0
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