In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 5 ( 2022-5-6), p. e1010181-
Abstract:
Gene body methylation (GBM) is an ancestral mode of DNA methylation whose role in development has been obscured by the more prominent roles of promoter and CpG island methylation. The wasp Nasonia vitripennis has little promoter and CpG island methylation, yet retains strong GBM, making it an excellent model for elucidating the roles of GBM. Here we show that N . vitripennis DNA methyltransferase 1a ( Nv-Dnmt1a ) knockdown leads to failures in cellularization and gastrulation of the embryo. Both of these disrupted events are hallmarks of the maternal-zygotic transition (MZT) in insects. Analysis of the embryonic transcriptome and methylome revealed strong reduction of GBM and widespread disruption of gene expression during embryogenesis after Nv-Dnmt1a knockdown. Strikingly, there was a strong correlation between loss of GBM and reduced gene expression in thousands of methylated loci, consistent with the hypothesis that GBM directly facilitates high levels of transcription. We propose that lower expression levels of methylated genes due to reduced GBM is the crucial direct effect of Nv-Dnmt1 knockdown. Subsequently, the disruption of methylated genes leads to downstream dysregulation of the MZT, culminating in developmental failure at gastrulation.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010181
DOI:
10.1371/journal.pgen.1010181.g001
DOI:
10.1371/journal.pgen.1010181.g002
DOI:
10.1371/journal.pgen.1010181.g003
DOI:
10.1371/journal.pgen.1010181.g004
DOI:
10.1371/journal.pgen.1010181.g005
DOI:
10.1371/journal.pgen.1010181.t001
DOI:
10.1371/journal.pgen.1010181.s001
DOI:
10.1371/journal.pgen.1010181.s002
DOI:
10.1371/journal.pgen.1010181.s003
DOI:
10.1371/journal.pgen.1010181.s004
DOI:
10.1371/journal.pgen.1010181.s005
DOI:
10.1371/journal.pgen.1010181.s006
DOI:
10.1371/journal.pgen.1010181.s007
DOI:
10.1371/journal.pgen.1010181.s008
DOI:
10.1371/journal.pgen.1010181.s009
DOI:
10.1371/journal.pgen.1010181.s010
DOI:
10.1371/journal.pgen.1010181.s011
DOI:
10.1371/journal.pgen.1010181.s012
DOI:
10.1371/journal.pgen.1010181.s013
DOI:
10.1371/journal.pgen.1010181.s014
DOI:
10.1371/journal.pgen.1010181.s015
DOI:
10.1371/journal.pgen.1010181.s016
DOI:
10.1371/journal.pgen.1010181.s017
DOI:
10.1371/journal.pgen.1010181.s018
DOI:
10.1371/journal.pgen.1010181.s019
DOI:
10.1371/journal.pgen.1010181.s020
DOI:
10.1371/journal.pgen.1010181.s021
DOI:
10.1371/journal.pgen.1010181.r001
DOI:
10.1371/journal.pgen.1010181.r002
DOI:
10.1371/journal.pgen.1010181.r003
DOI:
10.1371/journal.pgen.1010181.r004
DOI:
10.1371/journal.pgen.1010181.r005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
Permalink