In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 11 ( 2022-11-9), p. e1010931-
Abstract:
African swine fever virus (ASFV) is causing a worldwide pandemic affecting the porcine industry and leading to important global economic consequences. The virus causes a highly lethal hemorrhagic disease in wild boars and domestic pigs. Lack of effective vaccines hampers the control of virus spread, thus increasing the pressure on the scientific community for urgent solutions. However, knowledge on the immune components associated with protection is very limited. Here we characterized the in vitro recall response induced by immune cells from pigs intranasally vaccinated with the BA71ΔCD2 deletion mutant virus. Vaccination conferred dose-dependent cross-protection associated with both ASFV-specific antibodies and IFNγ-secreting cells. Importantly, bulk and single-cell transcriptomics of blood and lymph node cells from vaccinated pigs revealed a positive feedback from adaptive to innate immunity. Indeed, activation of Th1 and cytotoxic T cells was concomitant with a rapid IFNγ-dependent triggering of an inflammatory response characterized by TNF-producing macrophages, as well as CXCL10-expressing lymphocytes and cross-presenting dendritic cells. Altogether, this study provides a detailed phenotypic characterization of the immune cell subsets involved in cross-protection against ASFV, and highlights key functional immune mechanisms to be considered for the development of an effective ASF vaccine.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010931
DOI:
10.1371/journal.ppat.1010931.g001
DOI:
10.1371/journal.ppat.1010931.g002
DOI:
10.1371/journal.ppat.1010931.g003
DOI:
10.1371/journal.ppat.1010931.g004
DOI:
10.1371/journal.ppat.1010931.g005
DOI:
10.1371/journal.ppat.1010931.g006
DOI:
10.1371/journal.ppat.1010931.g007
DOI:
10.1371/journal.ppat.1010931.s001
DOI:
10.1371/journal.ppat.1010931.s002
DOI:
10.1371/journal.ppat.1010931.s003
DOI:
10.1371/journal.ppat.1010931.s004
DOI:
10.1371/journal.ppat.1010931.s005
DOI:
10.1371/journal.ppat.1010931.s006
DOI:
10.1371/journal.ppat.1010931.s007
DOI:
10.1371/journal.ppat.1010931.s008
DOI:
10.1371/journal.ppat.1010931.s009
DOI:
10.1371/journal.ppat.1010931.s010
DOI:
10.1371/journal.ppat.1010931.s011
DOI:
10.1371/journal.ppat.1010931.s012
DOI:
10.1371/journal.ppat.1010931.s013
DOI:
10.1371/journal.ppat.1010931.s014
DOI:
10.1371/journal.ppat.1010931.s015
DOI:
10.1371/journal.ppat.1010931.s016
DOI:
10.1371/journal.ppat.1010931.s017
DOI:
10.1371/journal.ppat.1010931.s018
DOI:
10.1371/journal.ppat.1010931.s019
DOI:
10.1371/journal.ppat.1010931.r001
DOI:
10.1371/journal.ppat.1010931.r002
DOI:
10.1371/journal.ppat.1010931.r003
DOI:
10.1371/journal.ppat.1010931.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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