In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 80, No. 4 ( 2014-02-15), p. 1403-1410
Abstract:
The genomic DNA from four species of ixodid ticks in western Canada was tested for the presence of Rickettsiella by PCR analyses targeting the 16S rRNA gene. Eighty-eight percent of the Ixodes angustus ( n = 270), 43% of the I. sculptus ( n = 61), and 4% of the I. kingi ( n = 93) individuals examined were PCR positive for Rickettsiella , whereas there was no evidence for the presence of Rickettsiella in Dermacentor andersoni ( n = 45). Three different single-strand conformation polymorphism profiles of the 16S rRNA gene were detected among amplicons derived from Rickettsiella -positive ticks, each corresponding to a different sequence type. Furthermore, each sequence type was associated with a different tick species. Phylogenetic analyses of sequence data of the 16S rRNA gene and three other genes ( rpsA , gidA , and sucB ) revealed that all three sequence types were placed in a clade that contained species and pathotypes of the genus Rickettsiella . The bacterium in I. kingi represented the sister taxon to the Rickettsiella in I. sculptus , and both formed a clade with Rickettsiella grylli from crickets ( Gryllus bimaculatus ) and “ R. ixodidis ” from I. woodi . In contrast, the Rickettsiella in I. angustus was not a member of this clade but was placed external to the clade comprising the pathotypes of R. popilliae . The results indicate the existence of at least two new species of Rickettsiella : one in I. angustus and another in I. kingi and I. sculptus . However, the Rickettsiella strains in I. kingi and I. sculptus may also represent different species because each had unique sequences for all four genes.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.03564-13
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2014
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
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