GLORIA

GEOMAR Library Ocean Research Information Access

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    In: Tropical Plant Pathology, Springer Science and Business Media LLC, Vol. 47, No. 2 ( 2022-04), p. 287-297
    Type of Medium: Online Resource
    ISSN: 1983-2052
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2519866-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 2
    Online Resource
    Online Resource
    MDPI AG ; 2021
    In:  Horticulturae Vol. 7, No. 9 ( 2021-09-02), p. 277-
    In: Horticulturae, MDPI AG, Vol. 7, No. 9 ( 2021-09-02), p. 277-
    Abstract: Citrus species, including sweet oranges, grapefruits, pomelos, and lemons, are the most widely cultivated trees and consumed fruits worldwide. In citrus orchard management, the control of Huanglongbing (HLB) disease and its insect vector Diaphorina citri (Asian citrus psyllid, ACP) represents a major global challenge. Consumers have been increasingly pushing the citrus production chain toward a more sustainable system, including stringent measures to prevent the use of chemical pesticides. In recent years, biotechnological advances have offered safe and environmentally friendly alternatives for crop production. Technologies such as RNA interference (RNAi)-mediated gene silencing have emerged as innovative tools for agricultural pest management. Here, we provide an overview of RNAi as a promising approach for ACP control and discuss the associated challenges. Despite the availability of specific silencing sequences aimed at a target gene of the insect pest, the uptake of double-stranded RNA is limited in hemipteran insects. In this context, improved delivery methods, stability maintenance, and RNAi response are considered the factors contributing to the increased effectiveness of exogenous RNAi against hemipteran pests. These approaches can serve as potential tools for efficient ACP control.
    Type of Medium: Online Resource
    ISSN: 2311-7524
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2813983-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 3
    Online Resource
    Online Resource
    Associacao Prudentina de Educacao e Cultura (APEC) ; 2021
    In:  COLLOQUIUM AGRARIAE Vol. 17, No. 4 ( 2021-8-12), p. 83-101
    In: COLLOQUIUM AGRARIAE, Associacao Prudentina de Educacao e Cultura (APEC), Vol. 17, No. 4 ( 2021-8-12), p. 83-101
    Abstract: Climate change and population size records threaten food security. Therefore, the call for a more sustainable and efficient crop production has never been more urgent. Traditional plant breeding was one of the first successful approaches to expand cultivation areas and crop yield. Later, biotechnological tools and their products, such as genetically modified organisms containing exogenous DNA, further broadened the limits of agricultural results, yet bringing huge financial, bureaucratic, and public rejection hurdles. In the 90s, scientific advances brought the opportunity to drive mutations using engineered nucleases, and since 2013 CRISPR-Cas has emerged as the most practical toolkit to edit genomes. One of the most striking possibilities is to generate edited and non-transgenic plants. In this review, we present the working mechanism behind CRISPR-induced mutations and pinpoint the latest techniques developed, as well as its myriad of applications in agriculture. The enhancing scope of CRISPR ranges from introducing traits of agronomic interest – such as herbicide resistance, resistance/tolerance to biotic and abiotic stresses, and quality and durability of products – to accelerating plant breeding processes, including haploid induction, generating male-sterile lines, fixating hybrid vigor, and overcoming self-incompatibility. We also discuss regulatory issues surrounding edited plants and derived products around the world, challenges that must be overcome, and future prospects to harness all the potential of this amazing tool to guarantee the new crop production revolution.
    Type of Medium: Online Resource
    ISSN: 1809-8215
    URL: Issue
    URL: Issue
    Language: Unknown
    Publisher: Associacao Prudentina de Educacao e Cultura (APEC)
    Publication Date: 2021
    detail.hit.zdb_id: 2628994-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 4
    In: Pesquisa Agropecuária Brasileira, FapUNIFESP (SciELO), Vol. 57 ( 2022)
    Abstract: Resumo O objetivo deste trabalho foi verificar se a introgressão do gene AtAREB1 em dois germoplasmas-elite de soja, 'LS93-0375' e 'BMX Desafio RR', aumenta a tolerância dessas plantas ao deficit hídrico. As progênies F4 das duas cultivares-elite e da linhagem transgênica AtAREB1 (BR16-AtAREB1) e de seu background ('BR16') foram submetidas a deficit hídrico. Os bioensaios de deficit hídrico foram realizados em casa de vegetação, tendo-se utilizado as seis seguintes linhagens de soja: a geneticamente modificada BR16-AtAREB1 e seu background 'BR16'; 'LS93' e sua progênie F4, LS93-AtAREB1; e 'BMX Desafio RR' e sua progênie F4, Desafio-AtAREB1. Utilizou-se delineamento experimental de blocos completos com tratamentos casualizados, em arranjo fatorial 6x2, com os seis genótipos de soja e duas condições hídricas – controle (C) e tratamentos de deficit hídrico (WD) –, com nove repetições. Os genótipos de soja que contêm o gene AtAREB1 exibiram melhor desempenho fisiológico sob estresse hídrico e expressão alterada de genes responsivos à seca. A introgressão de AtAREB1 na soja aumenta a tolerância à seca, independentemente do background genético em que o gene foi introduzido.
    Type of Medium: Online Resource
    ISSN: 1678-3921 , 0100-204X
    Uniform Title: Tolerância à seca em cultivares-elite de soja com a introgressão do transgene AtAREB1
    Language: English , Portuguese
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2022
    detail.hit.zdb_id: 2053197-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 5
    In: Pesquisa Agropecuária Brasileira, FapUNIFESP (SciELO), Vol. 58 ( 2023)
    Abstract: Resumo O objetivo deste trabalho foi desenvolver um método para criar e validar sistemas CRISPR-Cas e diferentes gRNAs em embriões de soja (Glycine max). Dois genes modelo foram usados para mutação simples com um gRNA ou deleção parcial do gene com dois guias. Os gRNAs foram inseridos nos vetores de transformação CRISPR por uma enzima de restrição do tipo IIS ou por subclonagem e inserção do promotor + gRNA2 no vetor de transformação final, com uso do método clássico de clonagem por enzimas de restrição. Os vetores foram construídos com sucesso para um e dois gRNAs. A transformação transiente de soja por Agrobacterium foi realizada para testar a qualidade dos gRNAs e do próprio sistema (cassete de expressão). Detectaram-se mutação simples e deleção gênica nos embriões transformados após o enriquecimento do DNA por digestão seguida de reação em cadeia da polimerase e sequenciamento, o que indica que o sistema CRISPR-Cas e os guias estavam funcionando. Este protocolo pode ser usado para acelerar as estratégias de edição de genoma baseadas em CRISPR, para transformação genética em soja.
    Type of Medium: Online Resource
    ISSN: 1678-3921 , 0100-204X
    Uniform Title: Expressão transiente de CRISPR em soja para triagem simplificada de gRNA na planta
    Language: English , Portuguese
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2023
    detail.hit.zdb_id: 2053197-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 6
    In: Scientia Horticulturae, Elsevier BV, Vol. 249 ( 2019-04), p. 432-438
    Type of Medium: Online Resource
    ISSN: 0304-4238
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2019
    detail.hit.zdb_id: 2016351-4
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 7
    Online Resource
    Online Resource
    Wiley ; 2017
    In:  Soil Science Society of America Journal Vol. 81, No. 3 ( 2017-05), p. 546-555
    In: Soil Science Society of America Journal, Wiley, Vol. 81, No. 3 ( 2017-05), p. 546-555
    Abstract: Core Ideas Polymer‐coated urea reduced N leaching as compared with conventional urea. Climatic conditions during the lantana growth affected the N uptake and N‐use efficiency. These results can be used for water and fertilizer management for ornamental plants. This study can be used to minimize environmental impact caused by N leaching. Lantana ( Lantana camara L.) is a popular ornamental plant in seaside communities and an annual plant in hanging baskets. This species cultivated as an annual plant in containers needs to receive fertilizers. The nutrient management in containers can be made using conventional or controlled release fertilizers. The effects of ratios among conventional urea (CU) and polymer‐coated urea (PCU), and irrigation water rates applied by evapotranspiration (ET)‐based irrigation were studied on dry weight of biomass and N uptake of lantana, N leaching, water‐use efficiency (WUE), and N‐use efficiency (NUE). Six ratios between CU and PCU (expressed in percentages) and two irrigation rates were evaluated: 0:100, 25:75, 50:50, 75:25, 100:0, and 0:0 of CU/PCU; and water volume applied by 150% of reference evapotranspiration (ETo) and 200% of ETo‐based irrigation. Two trials were conducted with environmental conditions varying between them. With greater temperatures, the cumulative number of inflorescences increased with 100% of PCU as compared with 100% of CU applied in the trial. Polymer‐coated urea (100%) as compared with CU (100%) also reduced N leaching by 64.5 and 61.5% in the first and second trial, respectively. High water volume applied (200% of ETo) increased the dry weight of biomass, N uptake, and NUE. Water volume applied by 150% of ETo‐based irrigation resulted in a reduction of 53.5 and 58.5% of N leaching in the first and second trial, respectively. The response of WUE to ratios among CU and PCU and irrigation rates was not consistent, and was influenced by climatic conditions since WUE increased 26.6% in the second trial characterized for lower temperatures.
    Type of Medium: Online Resource
    ISSN: 0361-5995 , 1435-0661
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 241415-6
    detail.hit.zdb_id: 2239747-4
    detail.hit.zdb_id: 196788-5
    detail.hit.zdb_id: 1481691-X
    SSG: 13
    SSG: 21
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 8
    Online Resource
    Online Resource
    FapUNIFESP (SciELO) ; 2018
    In:  Revista Brasileira de Fruticultura Vol. 40, No. 4 ( 2018-08-27)
    In: Revista Brasileira de Fruticultura, FapUNIFESP (SciELO), Vol. 40, No. 4 ( 2018-08-27)
    Abstract: Resumo No Brasil, os viticultores têm trocado os porta-enxertos tradicionalmente utilizados por aqueles que conferem maior vigor à copa. O porta-enxeto altera a distribuição da fertilidade de gemas distribuídas nos ramos de videira e a expressão da mesma em produtividade. Portanto, essa alteração pode alterar o comprimento ideal de ramo no momento da poda. Diante disso, neste estudo, foram avaliadas a fertilidade de gemas, a produtividade e a qualidade da uva ‘Niagara Rosada’ enxertada em porta-enxertos: ‘IAC 766’, ‘IAC 572’, ‘IAC 313’, ‘IAC 571-6’ e ‘Riparia do Traviu’, sendo o porta-enxerto ‘IAC 766’ o mais utilizado no Estado de São Paulo, atualmente . As avaliações foram realizadas ao longo de três safras. Duas avaliações foram realizadas na temporada tradicional brasileira, e uma poda foi realizada no verão brasileiro, denominada “temporã” ou safrinha. Nos ciclos de produção tradicionais, a fertilidade de gemas foi avaliada da primeira à quarta gema, em 2014, e à quinta em 2015. No ciclo de produção de segunda safra ou temporã, a fertilidade de gemas foi avaliada a partir da quinta até a oitava gema. A produtividade e a qualidade dos frutos também foram avaliados ao longo dos três ciclos de produção. A elevada variabilidade dos resultados de fertilidade de gema de ‘Niagara Rosada’, combinada com estes porta-enxertos, mostraram que esta característica foi mais afetada pelas condições ambientais e características genéticas da variedade copa do que pelos porta-enxertos estudados. Não se observou efeito de porta-enxertos na produtividade e na qualidade de ‘Niagara Rosada’. Foram detectadas variações isoladas, e estas não são suficientes para confirmar a influência de porta-enxertos na variedade copa Niagara Rosada. Embora não tenha sido observado qualquer efeito dos porta-enxertos na fertilidade de gemas, na produtividade e na qualidade dos frutos, nas condições estudadas, todos os porta-enxertos são recomendados para cultivo de ‘Niagara Rosada’.
    Type of Medium: Online Resource
    ISSN: 1806-9967 , 0100-2945
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2018
    detail.hit.zdb_id: 2105182-3
    SSG: 7,36
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 9
    In: Journal of Economic Entomology, Oxford University Press (OUP), Vol. 113, No. 6 ( 2020-12-09), p. 2931-2940
    Abstract: The Asian citrus psyllid, Diaphorina citri Kuwayama, transmits the bacteria Candidatus Liberibacter associated with huanglongbing (HLB), a devastating disease of the citrus industry. The use of genetically modified plants is an alternative to control this vector. Conversely, technology based on RNA interference (RNAi) for silencing specific genes of a target insect could be attempted. This work evaluated the knockdown effect of the target genes calreticulin (DcCRT), laccase (DcLAC), and Snf7 (DcSnf7) by RNAi through feeding D. citri in Murraya paniculata leaves after the uptake of an aqueous solution with dsRNA homologous to each vector target gene. Confocal microscopy revealed the uptake of the fluorescent-labeled dsRNA by detached leaves and the symplastic movement, allowing the ingestion by the feeding insect. A reduction in the survival rate was observed only 144 h after the beginning of feeding with dsRNA targeting DcSnf7; however, no reduction in transcript accumulation. The knockdown of the DcCRT and DcLAC genes was detected only 12 and 96 h after insect feeding, respectively. Additionally, a reduction in amino acid excretion from insects fed with dsRNA targets to DcCRT and DcLAC was observed 120 h after the beginning of feeding. However, the effects of the dsRNAs tested here appear to be minimal, both at the transcriptional and phenotype levels. For most concentrations and time points, no effects were observed. Therefore, the knockdown of genes DcCRT, DcLAC, and DcSnf7 do not appear to have the potential to control of D. citri through RNAi-mediated gene silencing.
    Type of Medium: Online Resource
    ISSN: 0022-0493 , 1938-291X
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2020
    detail.hit.zdb_id: 2477182-X
    detail.hit.zdb_id: 2030999-5
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 10
    Online Resource
    Online Resource
    American Society for Horticultural Science ; 2019
    In:  HortScience Vol. 54, No. 12 ( 2019-12), p. 2224-2230
    In: HortScience, American Society for Horticultural Science, Vol. 54, No. 12 ( 2019-12), p. 2224-2230
    Abstract: Fertilizer management is an essential step in the production process, as it allows the plant to use its productive capacity to the fullest extent possible. Researchers have tested maximum nutrient use with reduced losses to the environment aiming to increase productivity with fewer environmental impacts. This study compared the effects of controlled-release fertilizers (CRFs) with water-soluble fertilizer (WSF) and clear water (control) on the growth and nutrient uptake of croton ( Codiaeum variegatum L.) and nitrogen leaching. The experiment was conducted with three replications and six treatments: two rates (1.5 g and 3.0 g per liter of substrate) of two CRFs [Osmocote Plus (15% N, 3.93% P, and 9.96% K) and Basacote (15% N, 3.49% P, and 9.96% K)], WSF, and clean water as control. All CRFs were applied before planting and WSF was supplied as nutrient solution through automated moisture sensor activated irrigation system. Plant growth (number of leaves, leaf area, stem height, root volume, and shoot and root dry weights) and total nutrient contents in the leaf tissue were evaluated every 30 days. Electrical conductivity (EC), pH, nitrate, ammonium, and total nitrogen contents were measured in the leached solution. Indeed, results showed that CRFs at a low rate provided similar development and quality of croton plants compared with WSF. Plant growth indicators were similar until 90 days after transplanting (DAT). After that, at 150 DAT, the highest values to number of leaves and leaf area occurred with WSF and with the lowest CRF rate as compared with the other treatments and control. The highest root volume was found with the WSF, which resulted in larger roots compared with the other treatments. These results showed WSF can be replaced by CRFs at low rates on croton growth. Moreover, according to the visual scale, the best treatments were WSF and Basacote at the low rate, where plants were bright, with multicolored leaves with prominent orange shades. However, CRFs maintained pH and EC within the recommended range for the growth of croton and reduced the nitrogen leaching from the pots.
    Type of Medium: Online Resource
    ISSN: 0018-5345 , 2327-9834
    Language: Unknown
    Publisher: American Society for Horticultural Science
    Publication Date: 2019
    detail.hit.zdb_id: 2040198-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...