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  • 1
    In: Agronomy, MDPI AG, Vol. 11, No. 6 ( 2021-06-04), p. 1150-
    Abstract: Salinity and alkalinity stresses are common in arid and semiarid climates. Both these stresses not only retard crop growth but also cause a severe reduction in yields. The present experiment was performed to investigate the morphological, physiological, biochemical, and genetic responses of two maize hybrids (FH-1231 and DK-6714) to salinity and alkalinity stresses. The treatments were comprised of salt stress (NaCl:Na2SO4 at a 9:1 ratio), alkaline stress (NaHCO3:Na2CO3 at a 9:1 ratio), and an unstressed control. The results indicated that salinity and alkalinity significantly reduced shoot fresh weight by 50% and 70%, root fresh weight by 38% and 50%, root dry weight by 69% and 93%, seedling length by 18% and 30%, number of leaves by 27% and 39%, and maximum leaf width by 17% and 24%, respectively, across the two hybrids compared with control, indicating that alkalinity had a greater effect than salinity. Likewise, both the stresses, particularly alkalinity, significantly decreased K+ ion accumulation and chlorophyll content and increased the lipid peroxidation rate, sodium (Na+) concentration, the hydrogen peroxide (H2O2) level, and the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). Salinity and alkalinity stresses also induced the expression levels of antioxidant genes (SOD, CAT, POD, APX); however, salinity showed less effect than alkalinity stress. Similarly, hybrid DK-6714 performed comparatively better than FH-1231 with regard to seedling growth, antioxidant activities, and biochemical attributes under stress conditions. Thus, DK-6714 is recommended as a suitable hybrid for soils affected with salt-alkalization.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
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  • 2
    In: Canadian Journal of Plant Science, Canadian Science Publishing, Vol. 101, No. 4 ( 2021-08-01), p. 517-524
    Abstract: This study aimed to evaluate the total phenol, flavonoid, and individual phenolic acid contents and the antioxidant and cytotoxic activities of methanol extracts from Ficus cordata ssp. salicifolia. Spectrophotometry and gas chromatography – mass spectrometry (GC–MS) were used for chemical analyses, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was conducted to determine the antioxidant activity. The cytotoxic activity was assessed against MCF7 (human breast adenocarcinoma), A2780 (human ovary adenocarcinoma), HT29 (human colon adenocarcinoma), and MRC5 (normal human fetal lung fibroblast) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The total content of phenols, flavonoids, and antioxidant activity of the studied dry leaf, stem bark, twig, and fruit extracts were between 13.14 and 22.94 mg·GAE g −1 dry weight, 8.03 and 12.72 mg·QE g −1 dry weight, and 75.59% and 83.96%, respectively. Ten individual phenolic acids were quantified in the studied plant parts. The prominent phenolic acid was p-hydroxybenzoic acid, followed by vanillic acid. Methanolic fruit extracts exhibited promising cytotoxic activity against MCF7 (IC 50 : 21.11 μg·mL −1 ). Additionally, A2780 cell lines responded very well to all the extracts, with an IC 50 from 8.16 to 13.72 μg·mL −1 , except for the fruit extract, which was 47.39 μg·mL −1 . The leaf extract showed significant cytotoxicity against HT29 cell lines, with an IC 50 of 8.81 μg·mL −1 . All extracts showed IC 50 values ranging from 24.68 to 56.15 μg·mL −1 on normal cells. Further examination of the plant parts in this study could identify compounds accounting for the observed antioxidant and cytotoxic activities and might represent potential novel anticancer drugs.
    Type of Medium: Online Resource
    ISSN: 0008-4220 , 1918-1833
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2021
    detail.hit.zdb_id: 2016989-9
    SSG: 12
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  • 3
    In: Plants, MDPI AG, Vol. 10, No. 7 ( 2021-07-01), p. 1345-
    Abstract: Maize is one of the most economically important cereal crops worldwide. Salinity coupled with waterlogging is a major challenge for successful crop production. Understanding the underlying mechanisms and impacts of individual and combined salinity and waterlogging stress on the morpho-physio-biochemical and molecular responses and oxidative metabolism of maize during stress and recovery periods is essential. The present study was carried out to assess the response of four hybrid maize cultivars viz. DK-6142, FH-1231, FH-949, and MALKA-2016 under individual and combined salinity and waterlogging conditions. The treatments comprised the control (no stress), NaCl (salinity with 10 dSm−1), WL (waterlogged conditions with 3 cm flooding), and NaCl + WL (combined salinity and waterlogging stress). The data regarding morpho-physiological attributes were collected at 22 days after sowing (DAS; stress phase) and 30 DAS (recovery phase). The results revealed that both stresses, either individually or in combination, substantially reduced the root-shoot length, root-shoot fresh and dry weights, leaf width, and the number of leaves per plant as well as the leaf chlorophyll (Chl) and carotenoids contents; however, the inhibitory effects were more severe in combined stresses than for individual stress factors in many cultivars. Both individual and combined stress conditions enhanced hydrogen peroxide (H2O2) accumulation, whereas the antioxidant enzyme activities, i.e., superoxide dismutase (SOD), peroxidase (POD) catalase (CAT), and ascorbate peroxidase (APX), remained higher under stress conditions compared to the control. The expression levels of antioxidant genes (CAT and POD) were also upregulated under stress conditions. All of the cultivars recovered better from individual stresses than combined stress conditions; however, the hybrid DK-6142 performed better than the other maize hybrids under stress conditions and showed faster recovery.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2704341-1
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  • 4
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2023
    In:  Journal of Umm Al-Qura University for Applied Sciences Vol. 9, No. 3 ( 2023-09), p. 317-324
    In: Journal of Umm Al-Qura University for Applied Sciences, Springer Science and Business Media LLC, Vol. 9, No. 3 ( 2023-09), p. 317-324
    Abstract: Cadmium is the most harmful soil pollutant due to its long biological half-life. In the present study, the effect of Cd on ammonium assimilation in Arabidopsis thaliana wild-type Col0 was investigated. Thirty-day-old Arabidopsis seedlings were exposed to 20 µM CdCl 2 during different exposure times (0, 1, 2, 3, and 7 days). Seedling growth decreased under Cd stress mainly after 7 days of Cd exposure. Cd stress caused a gradual decrease in soluble leaf protein and induced an increase in leaf ammonium and the content of free amino acids such as glutamine (Gln), glutamate (Glu), asparagine (Asn), and proline (Pro), which may be related to the increase in protease activity. The results showed that the activities of Glutamine Synthetase (GS) and glutamate dehydrogenase (GDH) were inversely related. Cd stress led to an increase in GDH activity, whereas GS activity decreased. GDH activity on polyacrylamide gels showed that Cd induced both β- and α-enriched isoforms. Therefore, this study confirms that the ammonium assimilation process plays an important role for plants in adapting to Cd stress.
    Type of Medium: Online Resource
    ISSN: 2731-6734 , 1658-8185
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
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  • 5
    In: Annual Research & Review in Biology, Sciencedomain International, ( 2020-08-17), p. 79-88
    Abstract: This study was aimed to investigate the functional properties of peanut seeds, and physicochemical, nutritional and sensory analysis of processed peanut milk. Standard methods were used in this research to determine the functional properties and proximate composition, while minerals were determined by a flame photometer and colorimeter. Firstly, the functional proprieties of the whole and defatted kernel of peanut seeds were studied, and the best results were found in the defatted sample. Peanut milk samples were divided into four samples depends on the percentage of powder milk addition (3, 6, 9 and 12%) as well as the control sample without addition. The pH, total soluble solids, viscosity, and density of samples were (6.2-6.5, 6-16%, 3-8 mPa/s, and 1.01-1.05 g/cm3, respectively), increased with the increasing of milk powder. About the proximate analysis, the highest values of ash, protein, fat, total carbohydrates (0.8, 6.3, 6.3, and 7.5%, respectively) were found in the sample of 12% powder milk. Sodium, potassium, and calcium in the samples (3, 6, 9 and 12%) were found in the range 38-58, 55-89, and 10-18 mg/100 g, respectively. Sensory evaluation results revealed great acceptance for peanut milk made with 3% powder milk addition, while we're not found a significant difference in the color and taste of all samples and control. This study proved the possibility of using Sudanese peanut as a milk substitute which was found to be a good source of nutrients. This study suggested using peanut milk as a milk substitute, and in yogurt manufacture, future work should aim at studying optimum storage conditions and stable packaging requirements.
    Type of Medium: Online Resource
    ISSN: 2347-565X
    Language: Unknown
    Publisher: Sciencedomain International
    Publication Date: 2020
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  • 6
    In: The Lancet, Elsevier BV, Vol. 400, No. 10363 ( 2022-11), p. 1607-1617
    Type of Medium: Online Resource
    ISSN: 0140-6736
    RVK:
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
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    detail.hit.zdb_id: 3306-6
    detail.hit.zdb_id: 1476593-7
    SSG: 5,21
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  • 7
    Online Resource
    Online Resource
    MDPI AG ; 2021
    In:  Plants Vol. 10, No. 2 ( 2021-02-03), p. 289-
    In: Plants, MDPI AG, Vol. 10, No. 2 ( 2021-02-03), p. 289-
    Abstract: Amino acid transporters (AATs) are integral membrane proteins and have several functions, including transporting amino acids across cellular membranes. They are critical for plant growth and development. This study comprehensively identified AAT-encoding genes in tomato (Solanum lycopersicum), which is an important vegetable crop and serves as a model for fleshy fruit development. In this study, 88 genes were identified in the S. lycopersicum genome and grouped into 12 subfamilies, based on previously identified AATs in Arabidopsis, rice (Oryza sativa), and potato (Solanum tuberosum) plants. Chromosomal localization revealed that S. lycopersicum AAT (SlAAT) genes are distributed on the 12 S. lycopersicum chromosomes. Segmental duplication events contribute mainly to the expansion of SlAAT genes and about 32% (29 genes) of SlAAT genes were found to originate from this type of event. Expression profiles of SlAAT genes in various tissues of S. lycopersicum using RNA sequencing data from the Tomato Functional Genomics Database showed that SlAAT genes exhibited tissue-specific expression patterns. Comprehensive data generated in this study will provide a platform for further studies on the SlAAT gene family and will facilitate the functional characterization of SlAAT genes.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2704341-1
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  • 8
    In: Plants, MDPI AG, Vol. 12, No. 17 ( 2023-08-28), p. 3076-
    Abstract: Trehalose, a nonreducing disaccharide, has been linked to plant growth and development as well as stress response. The enzyme trehalose-6-phosphate phosphatase (TPP) plays a crucial role in the production of trehalose in higher plants. This study identified a total of seven TPP family genes within the pomegranate species (PgTPP1–PgTPP7). Three subgroups of the seven PgTPPs were identified through phylogenetic analysis. The gene length, coding sequence (CD) length, and chromosomal location of the PgTPP genes were studied. In addition, the PgTPP proteins’ length, isoelectric point (Ip), grand average of hydropathicity (GRAVY), conserved domains, conserved motifs, synteny, and phylogenetic relationships with Arabidopsis and tomato TPP proteins were examined. The cis-acting elements in the promoter region and the expression of the PgTPP genes under abscisic acid (ABA)-mediated drought stress as well as the differences in expression in the root, flower, and leaf tissues were also assessed. The PgTPP2 and PgTPP5 genes are involved in the response to abscisic-acid-mediated drought stress, as shown by drought-mediated stress transcriptomes. The PgTPP1 and PgTPP2 genes were expressed only in floral tissue and roots, respectively. The remaining PgTPPs did not exhibit any significant alterations in gene expression in roots, flowers, or leaves. The current study has the potential to provide a comprehensive understanding of the biological characteristics of PgTPP proteins in various developmental processes and their role in the pomegranate plant’s response to different stressors. However, further research is required to explore their precise biological role. Hence, conducting a comprehensive functional validation study on PgTPPs could contribute to the development of stress-resistant agricultural cultivars.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2704341-1
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  • 9
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2023
    In:  Euro-Mediterranean Journal for Environmental Integration Vol. 8, No. 1 ( 2023-03), p. 161-165
    In: Euro-Mediterranean Journal for Environmental Integration, Springer Science and Business Media LLC, Vol. 8, No. 1 ( 2023-03), p. 161-165
    Type of Medium: Online Resource
    ISSN: 2365-6433 , 2365-7448
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2843155-8
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  • 10
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2021
    In:  Planta Vol. 253, No. 1 ( 2021-01)
    In: Planta, Springer Science and Business Media LLC, Vol. 253, No. 1 ( 2021-01)
    Type of Medium: Online Resource
    ISSN: 0032-0935 , 1432-2048
    RVK:
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 1463030-8
    SSG: 12
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