In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 12 ( 2021-10-5)
Abstract:
Acinetobacter baumannii is a multidrug-resistant pathogen that represents a serious threat to global health. A. baumannii possesses a wide range of virulence factors that contribute to the bacterial pathogenicity. Among them, the siderophore acinetobactin is one of the most important, being essential for the development of the infection. In this study we performed an in-depth analysis of the acinetobactin cluster in the strain A. baumannii ATCC 17978. For this purpose, nineteen individual isogenic mutant strains were generated, and further phenotypical analysis were performed. Individual mutants lacking the biosynthetic genes entA, basG , basC , basD , and basB showed a significant loss in virulence, due to the disruption in the acinetobactin production. Similarly, the gene bauA , coding for the acinetobactin receptor, was also found to be crucial for the bacterial pathogenesis. In addition, the analysis of the Δ basJ/ Δ fbsB double mutant strain demonstrated the high level of genetic redundancy between siderophores where the role of specific genes of the acinetobactin cluster can be fulfilled by their fimsbactin redundant genes. Overall, this study highlights the essential role of entA , basG , basC , basD , basB and bauA in the pathogenicity of A. baumannii and provides potential therapeutic targets for the design of new antivirulence agents against this microorganism.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2021.752070
DOI:
10.3389/fmicb.2021.752070.s001
DOI:
10.3389/fmicb.2021.752070.s002
DOI:
10.3389/fmicb.2021.752070.s003
DOI:
10.3389/fmicb.2021.752070.s004
DOI:
10.3389/fmicb.2021.752070.s005
DOI:
10.3389/fmicb.2021.752070.s006
DOI:
10.3389/fmicb.2021.752070.s007
DOI:
10.3389/fmicb.2021.752070.s008
DOI:
10.3389/fmicb.2021.752070.s009
DOI:
10.3389/fmicb.2021.752070.s010
DOI:
10.3389/fmicb.2021.752070.s011
DOI:
10.3389/fmicb.2021.752070.s012
DOI:
10.3389/fmicb.2021.752070.s013
DOI:
10.3389/fmicb.2021.752070.s014
DOI:
10.3389/fmicb.2021.752070.s015
DOI:
10.3389/fmicb.2021.752070.s016
DOI:
10.3389/fmicb.2021.752070.s017
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2587354-4
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