In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 4 ( 2023-4-3), p. e0282742-
Abstract:
A new sunflower oil-chitosan decorated fly ash (sunflower oil/FA-CSNPs) bionanocomposite film was synthesized using the extract of Litopenaeus vannamei (White shrimp) and evaluated as an antibacterial and immunomodulatory agent. Fly ash-chitosan nanoparticles were produced by using chitosan (CS) isolated from white shrimp extract, glacial acetic acid and sodium tripolyphosphate solution as cross-linkage. The ultrafine polymeric sunflower oil-CS film was fabricated by treating fly ash-chitosan nanoparticles with sunflower oil in glacial acetic acid under continuous stirring for 24 h. The nanostructure of the fabricated polymeric film was confirmed and characterized by different microscopic and spectroscopic approaches. The surface morphology of pre-synthesized bionanocomposite film was found to be homogenous, even and without cracks and pores. The crystallinity of formed bionanocomposite film was noticed at angles (2θ) at 12.65°, 15.21°, 19.04°, 23.26°, 34.82°, and 37.23° in the XRD spectrum. The fabricated film displayed excellent stability up to 380 ⁰C. The formed sunflower oil/FA-CSNPs bionanocomposite film showed promising antibacterial towards Bacillus subtilis with highest zone of inhibition of 34 mm and Pseudomonas aeruginosa with zone of inhibition of 28 nm. The as-synthesized bionanocomposite film exhibited highest cell viability effect (98.95%), followed by FA-CSNPs (83.25%) at 200 μg mL -1 concentrations. The bionanocomposite film exerted notable immunomodulatory effect by promoting phagocytosis and enhancing the production of cytokines (NO, IL-6, IL-1β, and TNF-α) in macrophage-derived RAW264.7 cell line.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0282742
DOI:
10.1371/journal.pone.0282742.g001
DOI:
10.1371/journal.pone.0282742.g002
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10.1371/journal.pone.0282742.g003
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10.1371/journal.pone.0282742.g004
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10.1371/journal.pone.0282742.g005
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10.1371/journal.pone.0282742.g006
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10.1371/journal.pone.0282742.g007
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10.1371/journal.pone.0282742.g008
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10.1371/journal.pone.0282742.g009
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10.1371/journal.pone.0282742.g010
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10.1371/journal.pone.0282742.g011
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10.1371/journal.pone.0282742.g012
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10.1371/journal.pone.0282742.g013
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10.1371/journal.pone.0282742.g014
DOI:
10.1371/journal.pone.0282742.g015
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10.1371/journal.pone.0282742.g016
DOI:
10.1371/journal.pone.0282742.t001
DOI:
10.1371/journal.pone.0282742.t002
DOI:
10.1371/journal.pone.0282742.s001
DOI:
10.1371/journal.pone.0282742.s002
DOI:
10.1371/journal.pone.0282742.r001
DOI:
10.1371/journal.pone.0282742.r002
DOI:
10.1371/journal.pone.0282742.r003
DOI:
10.1371/journal.pone.0282742.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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