In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 33, No. 1 ( 1995-01), p. 205-208
Abstract:
To determine the sensitivity of a nested PCR procedure for detecting human immunodeficiency virus type 1 DNA in clinical specimens, 553 peripheral blood mononuclear cell samples obtained from 268 human immunodeficiency virus type 1-seropositive subjects were assayed by use of two independent primer sets for each sample. Overall, 1,088 of 1,106 (98.37%) reactions were positive. Investigation of the negative reactions showed that a low viral burden in some infected subjects, rather than primer-template mismatches, was the primary cause for the false-negative PCR results.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/jcm.33.1.205-208.1995
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1995
detail.hit.zdb_id:
1498353-9
detail.hit.zdb_id:
390499-4
SSG:
12
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