In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2022-8-26), p. e0273547-
Abstract:
Radiotherapy as an anti-tumor treatment can stimulate the immune system. However, irradiated tumor cells express CD47 to escape the anti-tumor immune response. Anti- CD47 Immunotherapy is a possible way to tackle this problem. This study evaluated the effect of single high dose radiotherapy combined with an anti-CD47 monoclonal antibody (αCD47 mAb) in CT26 tumor‐bearing BALB/c mice. We assessed the tumors volume and survival in mice 60 days after tumor implantation. Also, immune cell changes were analyzed by flow cytometry in tumors, lymph nodes, and spleen. Combination therapy enhanced the anti-tumor response in treated mice by increasing CD8+ T cells and M1 macrophages and decreasing M2 macrophages and myeloid-derived suppressor cells (MDSCs) in the tumor microenvironment (TME). Also, our results showed that combination therapy increased survival time in mice compared to other groups. Furthermore, tumor volumes remarkably decreased in mice that received a single high dose RT plus αCD47 mAb. In conclusion, we showed that combining RT and αCD47 mAb improved the immune cell population in TME, regressed tumor growth, and increased survival in tumor-bearing mice.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0273547
DOI:
10.1371/journal.pone.0273547.g001
DOI:
10.1371/journal.pone.0273547.g002
DOI:
10.1371/journal.pone.0273547.g003
DOI:
10.1371/journal.pone.0273547.g004
DOI:
10.1371/journal.pone.0273547.g005
DOI:
10.1371/journal.pone.0273547.g006
DOI:
10.1371/journal.pone.0273547.t001
DOI:
10.1371/journal.pone.0273547.s001
DOI:
10.1371/journal.pone.0273547.s002
DOI:
10.1371/journal.pone.0273547.r001
DOI:
10.1371/journal.pone.0273547.r002
DOI:
10.1371/journal.pone.0273547.r003
DOI:
10.1371/journal.pone.0273547.r004
DOI:
10.1371/journal.pone.0273547.r005
DOI:
10.1371/journal.pone.0273547.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
Permalink