In:
Pharmacia, Pensoft Publishers, Vol. 69, No. 4 ( 2022-12-16), p. 1075-1080
Abstract:
In December 2019, the World Health Organization was informed of an outbreak of pneumonia of unknown etiology in Wuhan, Hubei Province, China. On January 7, 2020, a new type of coronavirus was isolated, with the WHO later officially calling it “COVID-19” and the International Committee on Taxonomy of Viruses naming the virus “SARS-CoV-2”. On January 30, 2020, the WHO declared the severe acute respiratory syndrome coronavirus 2 outbreak a public health emergency of international concern, making it an unprecedented global public health challenge. From a scientific and literary reference, it was established that the main drugs in the therapy with Covid-19 are Dexamethasone and Levofluoxetine. For this reason, we pay attention to the analysis of these two medicinal products. In the literature, we did not find an analysis of a combination of Dexamethasone and Levofloxacin. Development and validation of a highperformance liquid chromatographic analytical procedure for simultaneously determining Dexamethasone and Levofloxacin in a synthetic mixture is described in this paper. The separation was made with a LiChrosorb RP 18 (250 × 4.6 mm) column, at 25 °C temperature,with isocratic mode andmobile phase,containingt acetonitrile and woter (70-30v/v). Eluent was monitored at 254 nm and the flow rate was 1.0 ml/min. Dexamethasone and Levofluoxetine were effectively separated with retention time (tr) of 4.69 min and 14.51 min,respectively,with in the selected chromatographic conditions.The method was validated for analytical parameters: specificity, linearity, precision, accuracy,andlimits of detection and quantitation. The calibration curves were linear inthe concentration range of 12.5 to 100.0 μg/ml for Dexamethasone and Levofloxacin, and the regression coefficientswere more than 0.999. For Dexamethasone and Levofloxacin the recovery was 100.01% and 100.04%, respectively. This analytical procedure is applicable for the quality control of drug formulations.
Type of Medium:
Online Resource
ISSN:
2603-557X
,
0428-0296
DOI:
10.3897/pharmacia.69.e97779
DOI:
10.3897/pharmacia.69.e97779.chart1
DOI:
10.3897/pharmacia.69.e97779.chart2
DOI:
10.3897/pharmacia.69.e97779.figure1
DOI:
10.3897/pharmacia.69.e97779.figure2
DOI:
10.3897/pharmacia.69.e97779.figure4
DOI:
10.3897/pharmacia.69.e97779.figure3
DOI:
10.3897/pharmacia.69.e97779.figure5
Language:
Unknown
Publisher:
Pensoft Publishers
Publication Date:
2022
detail.hit.zdb_id:
3010005-7
SSG:
15,3
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