In:
Proceedings, annual meeting, Electron Microscopy Society of America, Cambridge University Press (CUP), Vol. 27 ( 1969-08), p. 414-415
Abstract:
Only phosphotungstic acid (PTA) has been demonstrated to have a reasonably specific affinity for elastin in electron microscopy. However phosphotungstic acid is effective as a specific stain for elastin only at very acid pH. If the pH is increased, PTA is no more a specific stain for elastin, instead it acts as a general protein stain. Therefore at low pH only elastin is visible and one is unable to study the morphological relationships of the elastic components to their surrounding structures due to the virtual absence of contrast in them. Tetraphenylporphine sulfonate (TPPS) has, been shown to have a specific, affinity for elastic tissues of the body in fluorescence microscopy. However TPPS does not impart electron density to this tissue and thus is not suitable for electron microscopic studies. Therefore tetraphenylporphine sulfonate was complexed with various heavy metals in order to use it as a specific stain for elastin in electron microscopy.
Type of Medium:
Online Resource
ISSN:
0424-8201
,
2690-1315
DOI:
10.1017/S0424820100063986
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
1969
SSG:
11
Permalink