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  • 1
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    Online Resource
    Immunomodulatory, Apoptosis Induction and Antitumor Activities of Aqueous and Methanolic Extract of Calvatia Craniiformis in Mice Transfected with Murine Hepatocellular Carcinoma Cells
    Scientific Foundation SPIROSKI ; 2018
    In:  Open Access Macedonian Journal of Medical Sciences Vol. 6, No. 7 ( 2018-07-16), p. 1206-1214
    Details
    In: Open Access Macedonian Journal of Medical Sciences, Scientific Foundation SPIROSKI, Vol. 6, No. 7 ( 2018-07-16), p. 1206-1214
    Abstract: OBJECTIVES: To evaluate the Immunomodulatory, apoptosis induction and antitumor effects of aqueous and methanolic extracts of Calvatia craniiformis regarding the size of tumour mass, caspase-8 expression and apoptotic index (AI%) in mice transfected with murine hepatocellular carcinoma cell line (H22) as an experimental therapeutic system for human hepatocellular carcinoma.MATERIAL AND METHODS: Forty-eight Balb/C albino mice were transfected in legs with H22 cells. Tumour size was measured twice a week. Caspase-8 protein expression and apoptotic index determination evaluated by Immunohistochemistry.RESULTS: Tumor size significantly differed between the two groups of mice transfected with H22 cells; the first was treated with C. craniiformis aqueous extract (0.3, 0.6, 1.2) mg/kg and the second group was treated with C. craniiformis methanolic extract (0.25, 0.5, 1.0) mg/kg compared with control group. The inhibitory activity of aqueous and methanolic extracts was dose and duration dependent. The size of the tumour mass was reduced up to 87.9% when treated with 1.2 mg/kg aqueous extract and 1 mg/kg for methanolic extract. Caspase-8 expression was increased in a dose-dependent manner among H22 bearing mice treated with C. craniiformis aqueous extract (0.3, 0.6, 1.2) mg/kg. At 0.3 mg/kg, the intensity of expression was strong in (33.33%) and very strong in (66.67%). While at 0.6 mg/kg and 1.2 mg/kg the intensity of expression was strong in (33.33%) and very strong in (100%) with a significant difference (P ≤ 0.001). H22 bearing mice treated with (0.25, 0.5, 1.0) mg/kg C. craniiformis methanolic extract shows increased caspase-8 expression in a dose-dependent manner. At 0.25 mg/kg, the intensity of expression was strong in (33.33%) and very strong in (66.67%). While at 0.5 mg/kg, the intensity of expression was strong in (33.33%) and very strong in (100%). At 1.0 mg/kg, the intensity of expression was strong in (16.67%) and very strong in (83.33%) with significant difference (P ≤ 0.001). AI% of H22 bearing mice treated with C. craniiformis aqueous and methanolic extracts were significantly increased (P ≤ 0.05) compared with the untreated control group. No significant difference was reported in AI% between aqueous and methanolic extracts treated groups.CONCLUSIONS: Extracts of C. craniiformis were highly efficient in tumour growth inhibition, causing a reduction in the tumour size clinically and increase the expression of caspase-8 gene product in tumour tissue, causing increase apoptotic index of H22 cells taken from the legs of inoculated mice leading to loss of legs due to bone necrosis. Antitumor activity of C. craniiformis aqueous, and the methanolic extract was dose and duration dependent.
    Type of Medium: Online Resource
    ISSN: 1857-9655
    URL: Article
    DOI: 10.3889/oamjms.2018.275
    Language: Unknown
    Publisher: Scientific Foundation SPIROSKI
    Publication Date: 2018
    detail.hit.zdb_id: 3032255-8
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  • 2
    Online Resource
    Online Resource
    Evaluation of toxigenic and non-toxigenic fungal contaminants in poultry feed in Diyala province, Iraq
    Africa Health Research Organization ; 2020
    In:  Annals of Tropical Medicine and Public Health Vol. 23, No. 24 ( 2020)
    Details
    In: Annals of Tropical Medicine and Public Health, Africa Health Research Organization, Vol. 23, No. 24 ( 2020)
    Type of Medium: Online Resource
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.36295/ASRO.2020
    DOI: 10.36295/ASRO
    DOI: 10.36295/ASRO.2020.232418
    Language: Unknown
    Publisher: Africa Health Research Organization
    Publication Date: 2020
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  • 3
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    Online Resource
    Toxicopathological, Cytogenetic Effects of Acetothioamide on Female Albino Mice Reproductive System
    Scientific Foundation SPIROSKI ; 2019
    In:  Open Access Macedonian Journal of Medical Sciences Vol. 7, No. 23 ( 2019-12-13), p. 3925-3929
    Details
    In: Open Access Macedonian Journal of Medical Sciences, Scientific Foundation SPIROSKI, Vol. 7, No. 23 ( 2019-12-13), p. 3925-3929
    Abstract: AIM: To determine toxicopathological and cytogenetic effects of Acetothioamide (ATA) on the female reproductive system. METHODS: Twenty albino female mice were divided equally into two groups: the first group (control) fed with diet pellet. The second group (treated) were inoculated intraperitoneally with a single dose of ATA (100 mg/kg Bw) for 15 days. All mice were sacrificed at the end of the experiment and blood was collected for evaluation of (FSH and LH), serum peroxy nitrate radical concentration. Cytogenetic analysis (chromosomal aberration, micronuclei, mitotic index and blast index) and the histopathological examination on ovary and uterus were done. RESULTS: ATA causes significant reduction (p 〈 0.0001) for FSH, LH and serum peroxy nitrate radical concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were reported among treated females mainly in uterus tissue and ovary. CONCLUSION: ATA causes significant reduction for FSH, LH and serum peroxy nitrate concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were the main pathological changes in uterus tissue and ovary among treated females.
    Type of Medium: Online Resource
    ISSN: 1857-9655
    URL: Article
    DOI: 10.3889/oamjms.2019.858
    Language: Unknown
    Publisher: Scientific Foundation SPIROSKI
    Publication Date: 2019
    detail.hit.zdb_id: 3032255-8
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  • 4
    Online Resource
    Online Resource
    Immunomodulatory Effect of H. Pylori CagA Genotype and Gastric Hormones On Gastric Versus Inflammatory Cells Fas Gene Expression in Iraqi Patients with Gastroduodenal Disorders
    Scientific Foundation SPIROSKI ; 2016
    In:  Open Access Macedonian Journal of Medical Sciences Vol. 4, No. 3 ( 2016-06-05), p. 364-373
    Details
    In: Open Access Macedonian Journal of Medical Sciences, Scientific Foundation SPIROSKI, Vol. 4, No. 3 ( 2016-06-05), p. 364-373
    Abstract: AIM: To evaluate the Immunomodulatory effects of CagA expression; pepsinogen I, II & gastrin-17 on PMNs and lymphocytes Fas expression in inflammatory and gastric cells; demographic distribution of Fas molecule in gastric tissue and inflammatory cells.METHODS: Gastroduodenal biopsies were taken from 80 patients for histopathology and H. pylori diagnosis. Serum samples were used for evaluation of pepsinogen I (PGI); (PGII); gastrin-17 (G-17).RESULTS: Significant difference (p 〈 0.001) in lymphocytes & PMNs Fas expression; epithelial & lamina propria Fas localization among H. pylori associated gastric disorders. No correlation between grade of lymphocytes & PMNs Fas expression in gastric epithelia; lamina propria and types of gastric disorder. Significant difference (p 〈 0.001) in total gastric Fas expression, epithelial Fas; lamina propria and gastric gland Fas expression according to CagA, PGI; PGII; PGI/PGII; Gastrin-17. Total gastric Fas expression has significant correlation with CagA, PGII levels. Gastric epithelial and gastric lamina propria Fas expression have significant correlation with CagA, PGI; PGII levels. Significant difference (p 〈 0.001) was found in lymphocytes & PMNs Fas expression; epithelial & lamina propria localization of lymphocytes & PMNs Fas expression according to CagA, PGI; PGII; PGI/PGII; Gastrin-17. Lymphocytes Fas expression have correlation with PGI, PGII, PGI/PGII. PMNs Fas expression have correlation with PGI, PGII.CONCLUSION: Fas gene expression and localization on gastric and inflammatory cells affected directly by H. pylori CagA and indirectly by gastric hormones. This contributes to progression of various gastric disorders according to severity of CagA induced gastric pathology and gastric hormones disturbance throughout the course of infection and disease.
    Type of Medium: Online Resource
    ISSN: 1857-9655
    URL: Article
    DOI: 10.3889/oamjms.2016.032
    Language: Unknown
    Publisher: Scientific Foundation SPIROSKI
    Publication Date: 2016
    detail.hit.zdb_id: 3032255-8
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  • 5
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    Online Resource
    Phenotypic Identification and Molecular Characterization of Malassezia Spp. Isolated from Pityriasis Versicolor Patients with Special Emphasis to Risk Factors in Diyala Province, Iraq
    Scientific Foundation SPIROSKI ; 2019
    In:  Open Access Macedonian Journal of Medical Sciences Vol. 7, No. 5 ( 2019-02-27), p. 707-714
    Details
    In: Open Access Macedonian Journal of Medical Sciences, Scientific Foundation SPIROSKI, Vol. 7, No. 5 ( 2019-02-27), p. 707-714
    Abstract: AIM: The main objective is isolation and molecular characterisation of Malassezia spp. from pityriasis versicolor (PV) patients with special emphasis to risk factors in Diyala province, Iraq. METHODS: Fifty patients (32 males and 18 females) presented with PV, the age ranged (15-45) years were included. Direct wet mount using KOH 10%, culture of skin scraping and PCR were used for confirmatory diagnosis. RESULTS: Malassezia spp. was isolated from (54%) of skin scraping; M. furfur (32%); M. pachydermatis (8%) and M. globosa (14%). The age group (15-22) years were frequently exposed to Malassezia infection. A significant inverse correlation was reported between age and exposure to Malassezia spp. Infection. Males were frequently exposed to Malassezia infection, (40%). A significant correlation was reported between gender and exposure to Malassezia spp. Infection. Females were at risk of getting Malassezia infection (2.619) time than males. Patient resident in the urban area frequently exposed to Malassezia infection, (34%). Patients resident in the rural area appears to be at risk of getting Malassezia infection (1.093) time than those in an urban area. Patient with good economic status was frequently exposed to Malassezia infection, (36%). Patients with middle economic status appear to be at risk of getting Malassezia infection (0.42) time than those with good economic status. Patients with primary education were frequently exposed to Malassezia infection, (22%). A significant correlation was reported between education level and exposure to Malassezia spp. Infection. No significant correlation was reported between economic status; type of job; source of water; contact with dogs and birds and Malassezia spp. Infection. CONCLUSION: M. furfur, M. pachydermatis and M. globosa represent the most common Malassezia spp. causing PV. Using of PCR is very critical to confirm the diagnosis of Malassezia spp. Malassezia infection inversely correlated with age and positively correlated with females gender and education. The residency in a rural area and middle economic status increase the possibility of infection. Infection was not affected by the source of water; job and contact with dogs and birds.
    Type of Medium: Online Resource
    ISSN: 1857-9655
    URL: Article
    DOI: 10.3889/oamjms.2019.128
    Language: Unknown
    Publisher: Scientific Foundation SPIROSKI
    Publication Date: 2019
    detail.hit.zdb_id: 3032255-8
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  • 6
    Online Resource
    Online Resource
    Potential Probiotics Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 Co-Aggregate with Clinical Isolates of Proteus mirabilis and Prevent Biofilm Formation
    Springer Science and Business Media LLC ; 2020
    In:  Probiotics and Antimicrobial Proteins Vol. 12, No. 4 ( 2020-12), p. 1471-1483
    Details
    In: Probiotics and Antimicrobial Proteins, Springer Science and Business Media LLC, Vol. 12, No. 4 ( 2020-12), p. 1471-1483
    Type of Medium: Online Resource
    ISSN: 1867-1306 , 1867-1314
    URL: Article
    DOI: 10.1007/s12602-020-09631-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2487792-X
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  • 7
    Online Resource
    Online Resource
    Evaluation of Clinicopathological and Risk Factors for Nonma-lignant H. Pylori Associated Gastroduodenal Disorders in Iraqi Patients
    Scientific Foundation SPIROSKI ; 2015
    In:  Open Access Macedonian Journal of Medical Sciences Vol. 3, No. 4 ( 2015-11-05), p. 645-654
    Details
    In: Open Access Macedonian Journal of Medical Sciences, Scientific Foundation SPIROSKI, Vol. 3, No. 4 ( 2015-11-05), p. 645-654
    Abstract: AIM: To determine the risk factors associated with H. pylori infection and possible correlation with clinicopathological parameters.MATERIAL AND METHODS: Gastroduodenal biopsies were examined by rapid urease test and Gram staining. Cag A cytotoxin was detected by in situ hybridization.RESULTS: Risk of H. pylori acquisition reported as following: Males have 1.38 fold, rural residents have 0.63 fold, Nonsmokers have 0.39 fold, mild smokers have 18 fold, and moderate smokers have 1.4 fold while heavy smokers have 1 fold. A person who’s in contact with animals has 1.52 fold risks. Illiterates and patients with primary education have 5.36 & 3 fold risk respectively. Patients under proton pump inhibitor (PPI) therapy have 1.02 fold. Patients under NSAID therapy have 3.48 fold while nonalcoholic Patients have 0.75 fold. Patients using tap water have 0.45 fold risk. H. pylori infection positively correlated with age, weight loss, and heartburn. H. pylori inversely correlated with endoscopic diagnosis, Cag A positivity, and education level. Cag A positivity correlated with animal contact and NSAID usage.CONCLUSIONS: Several life style factors, education, animal contact, using of PPI, and NSAIDs increase the risk of H. pylori infection. Weight loss and heartburn cardinal signs for H. pylori infection. Endoscopic diagnosis and clinicopathological parameters not strictly associated with Cag A positivity.
    Type of Medium: Online Resource
    ISSN: 1857-9655
    URL: Article
    DOI: 10.3889/oamjms.2015.113
    Language: Unknown
    Publisher: Scientific Foundation SPIROSKI
    Publication Date: 2015
    detail.hit.zdb_id: 3032255-8
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  • 8
    Online Resource
    Online Resource
    Evaluation of Possible Stress Factors Affecting Physiological Level of Gamma Interferon During First Six Months of Life in Healthy Calves
    ResearchersLinks Ltd ; 2019
    In:  Advances in Animal and Veterinary Sciences Vol. 7, No. 5 ( 2019)
    Details
    In: Advances in Animal and Veterinary Sciences, ResearchersLinks Ltd, Vol. 7, No. 5 ( 2019)
    Type of Medium: Online Resource
    ISSN: 2307-8316
    URL: Article
    DOI: 10.17582/journal.aavs/2019/7.5.370.377
    Language: Unknown
    Publisher: ResearchersLinks Ltd
    Publication Date: 2019
    detail.hit.zdb_id: 2727738-0
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  • 9
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    Online Resource
    The Accuracy of Elisa Versus Latex Agglutination Tests in Diagnosis of Rotavirus Acute Gastroenteritis and the Clinical Usefulness of C- Reactive Protein in Iraqi Children
    Scientific Foundation SPIROSKI ; 2016
    In:  South East European Journal of Immunology Vol. 2016, No. 2016 ( 2016-12-24), p. 1-5
    Details
    In: South East European Journal of Immunology, Scientific Foundation SPIROSKI, Vol. 2016, No. 2016 ( 2016-12-24), p. 1-5
    Type of Medium: Online Resource
    ISSN: 1857-9388
    URL: Journal
    URL: Article
    DOI: 10.3889/seejim
    DOI: 10.3889/seejim.2016.20008
    Language: Unknown
    Publisher: Scientific Foundation SPIROSKI
    Publication Date: 2016
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  • 10
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    Online Resource
    IMMUNOHISTOPATHOLOGICAL ROLE OF BCL2 AND P53 GENE EXPRESSION IN HELICOBACTER PYLORI CYTOTOXIN-ASSOCIATED GENE A POSITIVE VERSUS CYTOTOXIN-ASSOCIATED GENE A NEGATIVE ANTRAL PREDOMINANT NON-ATROPHIC GASTRITIS IN IRAQI PATIENTS
    Innovare Academic Sciences Pvt Ltd ; 2017
    In:  Asian Journal of Pharmaceutical and Clinical Research Vol. 10, No. 3 ( 2017-03-01), p. 142-
    Details
    In: Asian Journal of Pharmaceutical and Clinical Research, Innovare Academic Sciences Pvt Ltd, Vol. 10, No. 3 ( 2017-03-01), p. 142-
    Abstract: ABSTRACTObjectives: To determine the role of Bcl2 and p53 gene expression in the pathogenesis of antral-predominant non-atrophic gastritis (APNG) accordingto Helicobacter pylori (HP) cytotoxin-associated gene A (CagA) status.Methods: Multiple antral biopsies were taken from 78 patients for rapid urease test, histopathology, Bcl2 and P53 immunohistochemistry and HPCagA in situ hybridization.Results: CagA was detected in 74.35% cases. There was no significant difference in Bcl2 expression among CagA+ and CagA− APNG cases. Significantdifferences in polymorphonuclear neutrophils (PMNs) and lymphocytes grades were detected among CagA+ and CagA− APNG cases. There was nosignificant correlation among patients’ age, inflammatory infiltrates, Bcl2, p53 expression. CagA has positive correlation with p53 (p=0.001), PMNsgrade (p=0.027), lymphocytes grade (p=0.003), inflammation intensity (p=0.006), and inflammation activity (p=0.007). Bcl2 has no significantcorrelation with p53, CagA, PMNs, and lymphocytes indexes. P53 expression has significant correlation with PMNs and lymphocyte grades (p=0.000),inflammation intensity (p=0.003), and inflammation activity (p=0.002). PMNs grade has positive correlation with lymphocyte grade, inflammationintensity, and activity (p=0.000). Lymphocyte grade has a significant correlation with inflammation intensity and activity (p=0.000). Inflammationintensity has a significant correlation with inflammation activity (p=0.000).Conclusions: CagA cytotoxin has direct effect on P53 gene and indirect effect on Bcl2 gene expression in APNG cases. Bcl2 and P53 expression donot get affected by patient’s age and gender. PMNs grade, lymphocytes grade, inflammation intensity, and inflammation activity affected directly byP53 and CagA cytotoxin expression and indirectly by Bcl2 expression. The balance of P53-Bcl2 pathways play a vital role in pathogenesis of HP andCagA-induced APNG.Keywords: Antral-predominant non-atrophic gastritis, Bcl2, p53, Helicobacter pylori, Cytotoxin-associated gene A.
    Type of Medium: Online Resource
    ISSN: 2455-3891 , 0974-2441
    URL: Article
    DOI: 10.22159/ajpcr.2017.v10i3.15622
    Language: Unknown
    Publisher: Innovare Academic Sciences Pvt Ltd
    Publication Date: 2017
    detail.hit.zdb_id: 2500247-8
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