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  • 1
    Online Resource
    Online Resource
    〈p〉Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of 〈em〉Salmonella 〈/em〉Serovars Isolated From Chickens〈/p〉
    Informa UK Limited ; 2019
    In:  Infection and Drug Resistance Vol. Volume 12 ( 2019-10), p. 3327-3342
    Details
    In: Infection and Drug Resistance, Informa UK Limited, Vol. Volume 12 ( 2019-10), p. 3327-3342
    Type of Medium: Online Resource
    ISSN: 1178-6973
    URL: Journal
    URL: Article
    DOI: 10.2147/IDR
    DOI: 10.2147/IDR.S217421
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2494856-1
    SSG: 15,3
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  • 2
    Online Resource
    Online Resource
    INTERACTION OF SELECTED LACTIC ACID BACTERIA AND YEAST IMPROVES THE QUALITY OF PEARL MILLET SOURDOUGH BREAD
    Slovak University of Agriculture in Nitra ; 2020
    In:  Journal of microbiology, biotechnology and food sciences Vol. 10, No. 1 ( 2020-08-01), p. 22-27
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 10, No. 1 ( 2020-08-01), p. 22-27
    Abstract: The effect of lactic acid bacteria and yeast interaction on the quality of pearl millet (PM) sourdough bread was investigated. Lactobacillus plantarum (A), Pediococcus pentosaceus (B), Lactobacillus pentosus (C), Saccharomyces cerevisiae (D) and Candida milleri (E) cultures were used singly and in combinations in sourdough fermentation. The proximate, physical and sensory properties of bread were evaluated using standard methods. The protein content ranged from 13.07% - 14.32%, ash (1.77% - 1.99%), fat (8.01% - 9.32%), crude fibre (0.77% - 1.25%), moisture (21.08% - 22.65%), carbohydrate (51.28%- 54.23%), loaf weight (75.28 g-108.32 g), loaf volume (89 cm3 – 130 cm3) and specific volume (1.0 cm3/g - 1.26 cm3/g). PMABE (Pearl Millet + Lb. plantarum + Lb. pentosus + C. milleri) was scored acceptable. The crude protein, fat, ash, fibre content and specific volume differed depending on the type of starters used in the development of sourdough bread. The use of co-cultures as starters could improves the physicochemical quality and acceptability of sourdough bread. Saccharomyces cerevisiae have been implicated in dough development, however, this study have shown that Candida milleri could also be a substitute in dough development in the pastry industry.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2020.10.1.22-27
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2020
    detail.hit.zdb_id: 2641525-2
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  • 3
    Online Resource
    Online Resource
    Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa
    Polish Society of Microbiologists ; 2020
    In:  Polish Journal of Microbiology Vol. 69, No. 4 ( 2020-01-01), p. 427-439
    Details
    In: Polish Journal of Microbiology, Polish Society of Microbiologists, Vol. 69, No. 4 ( 2020-01-01), p. 427-439
    Abstract: Bacterial biofilms have recently gained considerable interest in the food production and medical industries due to their ability to resist destruction by disinfectants and other antimicrobials. Biofilms are extracellular polymer matrices that may enhance the survival of pathogens even when exposed to environmental stress. The effect of incubation temperatures (25°C, 37°C, and 40°C) and Salmonella serotype on biofilm-forming potentials was evaluated. Previously typed Salmonella serotypes (55) isolated from the gut of chickens were accessed for biofilms formation using a standard assay. Salmonella Typhimurium ATCC 14028 TM and Salmonella Enteritidis ATCC 13076 TM (positive controls), Escherichia coli (internal control) and un-inoculated Luria Bertani (LB) broth (negative control) were used. The isolates formed no biofilm (11.86–13.56%), weak (11.86–45.76%), moderate (18.64–20.34%), strong biofilms (23.73–54.24%) across the various temperatures investigated. Serotypes, Salmonella Heidelberg and Salmonella Weltevreden were the strongest biofilm formers at temperatures (25°C, 37°C, and 40°C, respectively). The potential of a large proportion (80%) of Salmonella serotypes to form biofilms increased with increasing incubation temperatures but decreased at 40°C. Findings indicate that average temperature favours biofilm formation by Salmonella serotypes. However, the influence of incubation temperature on biofilm formation was greater when compared to serotype. A positive correlation exists between Salmonella biofilm formed at 25°C, 37°C and 40°C ( p ≥ 0.01). The ability of Salmonella species to form biofilms at 25°C and 37°C suggests that these serotypes may present severe challenges to food-processing and hospital facilities.
    Type of Medium: Online Resource
    ISSN: 2544-4646
    URL: Article
    DOI: 10.33073/pjm-2020-046
    Language: English
    Publisher: Polish Society of Microbiologists
    Publication Date: 2020
    detail.hit.zdb_id: 2234080-4
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Genome Sequence of Campylobacter fetus subsp. venerealis NW_ED23, Isolated from Bovine Sheath Wash
    American Society for Microbiology ; 2020
    In:  Microbiology Resource Announcements Vol. 9, No. 40 ( 2020-10)
    Details
    In: Microbiology Resource Announcements, American Society for Microbiology, Vol. 9, No. 40 ( 2020-10)
    Abstract: Campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis, which is mostly characterized by reproduction problems. The strain reported in this study was isolated from bull sheath wash in South Africa.
    Type of Medium: Online Resource
    ISSN: 2576-098X
    URL: Article
    DOI: 10.1128/MRA.00854-20
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2020
    detail.hit.zdb_id: 2968655-6
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  • 5
    Online Resource
    Online Resource
    Phenolic compounds suppress anthracnose decay by enhancing antifungal properties and biochemical defence responses in avocado fruit
    Springer Science and Business Media LLC ; 2022
    In:  Journal of Plant Pathology Vol. 104, No. 2 ( 2022-05), p. 711-720
    Details
    In: Journal of Plant Pathology, Springer Science and Business Media LLC, Vol. 104, No. 2 ( 2022-05), p. 711-720
    Type of Medium: Online Resource
    ISSN: 2239-7264
    URL: Article
    DOI: 10.1007/s42161-022-01085-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2212051-8
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  • 6
    Online Resource
    Online Resource
    Polyphasic Assessment of Aflatoxin Production Potential in Selected Aspergilli
    MDPI AG ; 2019
    In:  Toxins Vol. 11, No. 12 ( 2019-11-26), p. 692-
    Details
    In: Toxins, MDPI AG, Vol. 11, No. 12 ( 2019-11-26), p. 692-
    Abstract: This study investigated the aflatoxin production potentials of selected fungi using a polyphasic approach. Internally transcribed spacer region of the fungi was amplified using the polymerase chain reaction. Forty-five Aspergillus strains were further assessed for aflatoxin production using the conventional methods such as growth on yeast extract sucrose, β-cyclodextrin neutral red desiccated coconut agar (β-CNRDCA); expression of the aflatoxin regulatory genes and the use of both thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). A large proportion (82.22%) of the isolates harbored the Nor-1 gene while 55.56%, 68.89%, and 80% possessed the ver-1, omt-A, and aflR genes, respectively. All 100% the isolates harbored the aflJ gene. Twenty-three isolates were positive for aflatoxin production based on the yeast extract sucrose medium (YES) test; ammonium vapor test (51%), yellow pigment production (75.5%), and β-CNRDCA tests; and blue/green fluorescence (57.7%). Based on TLC detection 42.2% produced aflatoxins while in the HPLC, total aflatoxin (AFTOT) production concentrations ranged from 6.77–71,453 µg/g. Detectable aflatoxin B1 (AFB1) concentrations obtained from the HPLC ranged between 3.76 and 70,288 µg/g; 6.77 and 242.50 µg/g for aflatoxin B2 (AFB2); 1.87 and 745.30 µg/g for aflatoxin G1 (AFG1); and 1.67 and 768.52 µg/g for aflatoxin G2 (AFG2). AFTOT contamination levels were higher than European Union tolerable limits (4 µg/kg). The regression coefficient was one (R2 = 1) while significant differences exist in the aflatoxin concentrations of Aspergillus (p ≤ 0.05). This study reports the potentials of Aspergillus oryzae previously known as a non-aflatoxin producer to produce AFG1, AFG2, AFB1, and AFB2 toxins. Aspergillus species in feedlots of animals reared for food are capable of producing aflatoxins which could pose hazards to health.
    Type of Medium: Online Resource
    ISSN: 2072-6651
    URL: Article
    DOI: 10.3390/toxins11120692
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2518395-3
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  • 7
    Online Resource
    Online Resource
    Chitosan molecular weights affect anthracnose incidence and elicitation of defence-related enzymes in avocado (Persea americana) cultivar ‘Fuerte’
    Elsevier BV ; 2022
    In:  International Journal of Food Microbiology Vol. 366 ( 2022-04), p. 109561-
    Details
    In: International Journal of Food Microbiology, Elsevier BV, Vol. 366 ( 2022-04), p. 109561-
    Type of Medium: Online Resource
    ISSN: 0168-1605
    URL: Article
    DOI: 10.1016/j.ijfoodmicro.2022.109561
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2013748-5
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Analysis of Selected Mycotoxins in Maize from North-West South Africa Using High Performance Liquid Chromatography (HPLC) and Other Analytical Techniques
    MDPI AG ; 2021
    In:  Separations Vol. 8, No. 9 ( 2021-09-03), p. 143-
    Details
    In: Separations, MDPI AG, Vol. 8, No. 9 ( 2021-09-03), p. 143-
    Abstract: Contamination of foods by mycotoxins is linked to various health and economic implications. This study evaluated the incidence of mycotoxins in commercial and small-scale maize and evaluated potential health risks for consumers based on South African and international regulations. The sensitivity/specificity of HPLC over other analytical methods used was also ascertained. In total, 100 maize samples were analyzed using immuno-affinity column for extraction and clean-up, thin layer chromatography (TLC), HPLC, and enzyme linked immunosorbent assay (ELISA) for quantification. Results revealed that fumonisinB1 was the most contaminant mycotoxin in both small-scale and commercial samples with incidence rates of 100% and 98.6%, respectively. Aflatoxins contamination occurred at incidences of 26.7% in small-scale and 25.0% in commercial samples. Furthermore, ochratoxin A had high incidence rates of 97.8% and 93.0% and ranged from 3.60–19.44 µg/kg and 1.60–9.89 µg/kg, respectively, in small-scale and commercial samples, while ZEA occurred in 50% and 55% of small-scale and commercial samples, respectively. Results demonstrate that maize, especially from small-scale farmers, may contribute to dietary exposure to mycotoxins. Farmers and consumers should be alerted to the dangers of mycotoxins contamination in maize with resultant health risks. Additionally, HPLC method was also found to be more specific for mycotoxin detection than ELISA.
    Type of Medium: Online Resource
    ISSN: 2297-8739
    URL: Article
    DOI: 10.3390/separations8090143
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2869930-0
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  • 9
    Online Resource
    Online Resource
    Behaviour of Aspergillus parasiticus in aflatoxin production as influenced by storage parameters using response surface methodology approach
    Elsevier BV ; 2021
    In:  International Journal of Food Microbiology Vol. 357 ( 2021-11), p. 109369-
    Details
    In: International Journal of Food Microbiology, Elsevier BV, Vol. 357 ( 2021-11), p. 109369-
    Type of Medium: Online Resource
    ISSN: 0168-1605
    URL: Article
    DOI: 10.1016/j.ijfoodmicro.2021.109369
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2013748-5
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Efficacy of Lactic Acid Bacteria as a Biocontrol Agent against Anthracnose (Persea americana Miller) Decay in Avocado (Persea americana) cv Fuerte Fruit
    MDPI AG ; 2023
    In:  Agriculture Vol. 13, No. 2 ( 2023-01-22), p. 269-
    Details
    In: Agriculture, MDPI AG, Vol. 13, No. 2 ( 2023-01-22), p. 269-
    Abstract: Anthracnose disease is still a threat to avocado fruit quality, and the use of fungicide (Plochloraz®) for its control has generated safety concerns that necessitate the search for alternatives. Therefore, the efficiency of lactic acid bacteria (LAB) isolated from fresh fruits and vegetables as biocontrol agents against Colletotrichum gloeosporioides was investigated in this study. Weissella cibaria 21 (LAB 21), Leuconostoc pseudomesenteroides 56 (LAB 56), Weissella confusa 17 (LAB 17), Lactiplantibacillus plantarum 75 (LAB 75), and Lactiplantibacillus plantarum 171 (LAB 171) were evaluated in vitro as potential biocontrol agents to replace the Prochloraz® that is currently used in susceptible avocado (Persea americana Miller) Fuerte fruit. To confirm the biocontrol activity of the selected LAB strains, the antagonistic growth, spore germination, LAB recovery, nutrient competition, acid tolerance, and biofilm formation were assessed. In fruit treated with a LAB cell suspension, curatively inoculated with C. gloeosporioides, or naturally infected avocado cv Fuerte fruit, the epicatechin content and expression of defense-related genes (PAL, LOX, AVFADl 2–3, AVFAEL, and FLS) were compared with Prochloraz® and sterile water (control) treatments. With LAB 56, LAB 75, and LAB 21, significant inhibition of radial mycelial growth (MGI) ( 〉 90%) and spore germination (100%) was observed similar to those due to Prochloraz®. The MGI increased with a reduction in nutrient concentration. LAB strains reduced anthracnose disease incidence and severity compared with Prochloraz® and were the highest in LAB 21 and LAB 56. The LAB 21 and LAB 56 strains produced strong biofilms against C. gloeosporioides. In contrast to LAB 56, the control, and Prochloraz®, and LAB 21 had the highest epicatechin content (406 mg/g) and upregulated the PAL, AVFADl 2–3, AVFAEl, and FLS genes, thereby reducing the incidence of anthracnose in avocado fruit. As a result, LAB 21 suspensions can be used as an alternative to Prochloraz® in the control of anthracnose disease.
    Type of Medium: Online Resource
    ISSN: 2077-0472
    URL: Article
    DOI: 10.3390/agriculture13020269
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2651678-0
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