In:
Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 92, No. 2 ( 2009-03-01), p. 612-621
Abstract:
A sensitive method based on solid-phase extraction-liquid chromatography-tandem mass spectrometry interfaced with electrospray ionization (SPE-LC-MS/MS-ESI) was developed for the simultaneous determination of 8 banned nitroimidazole (NOZ) drugs including metronidazole (MNZ), ronidazole (RNZ), dimetridazole (DMZ), tinidazole, ornidazole, secnidazole, metronidazole-OH (MNZOH, the metabolite of MNZ), and 2-hydroxymethyl-1-methyl-5-nitroimidazole (HMMNI, the metabolite of RNZ and DMZ) in natural casings. After extraction with ethyl acetate and evaporation, the NOZs were reconstituted in ethyl acetate and purified on a strong cation-exchange SPE column, and then LC/MS/MS analysis was performed by positive ESI applying multiple reaction monitoring of 2 transition reactions for each compound. The method was validated according to the European Union requirements (Commission Decision 2002/657/EC). Specificity, linearity, decision limit (CC), detection capability (CC), accuracy, and precision were determined. Average recoveries of the 8 NOZs from natural animal casing fortified at 3 levels (0.1, 0.5, and 1 g/kg) ranged from 87.3 to 116.5. The calculated CC for NOZs ranged from 0.029 to 0.049 g/kg, and CC ranged from 0.049 to 0.083 g/kg. Repeatability was in the range of 3.3510.1, and within-laboratory reproducibility was & lt;10.3.
Type of Medium:
Online Resource
ISSN:
1060-3271
,
1944-7922
DOI:
10.1093/jaoac/92.2.612
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2009
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