In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 3 ( 2022-3-7), p. e1010340-
Abstract:
SARS-CoV-2 attaches to angiotensin-converting enzyme 2 (ACE2) to gain entry into cells after which the spike protein is cleaved by the transmembrane serine protease 2 (TMPRSS2) to facilitate viral-host membrane fusion. ACE2 and TMPRSS2 expression profiles have been analyzed at the genomic, transcriptomic, and single-cell RNAseq levels. However, transcriptomic data and actual protein validation convey conflicting information regarding the distribution of the biologically relevant protein receptor in whole tissues. To describe the organ-level architecture of receptor expression, related to the ability of ACE2 and TMPRSS2 to mediate infectivity, we performed a volumetric analysis of whole Syrian hamster lung lobes. Lung tissue of infected and control animals was stained using antibodies against ACE2 and TMPRSS2, combined with SARS-CoV-2 nucleoprotein staining. This was followed by light-sheet microscopy imaging to visualize their expression and related infection patterns. The data demonstrate that infection is restricted to sites containing both ACE2 and TMPRSS2, the latter is expressed in the primary and secondary bronchi whereas ACE2 is predominantly observed in the bronchioles and alveoli. Conversely, infection completely overlaps where ACE2 and TMPRSS2 co-localize in the tertiary bronchi, bronchioles, and alveoli.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010340
DOI:
10.1371/journal.ppat.1010340.g001
DOI:
10.1371/journal.ppat.1010340.g002
DOI:
10.1371/journal.ppat.1010340.g003
DOI:
10.1371/journal.ppat.1010340.g004
DOI:
10.1371/journal.ppat.1010340.g005
DOI:
10.1371/journal.ppat.1010340.g006
DOI:
10.1371/journal.ppat.1010340.g007
DOI:
10.1371/journal.ppat.1010340.s001
DOI:
10.1371/journal.ppat.1010340.s002
DOI:
10.1371/journal.ppat.1010340.s003
DOI:
10.1371/journal.ppat.1010340.s004
DOI:
10.1371/journal.ppat.1010340.s005
DOI:
10.1371/journal.ppat.1010340.s006
DOI:
10.1371/journal.ppat.1010340.s007
DOI:
10.1371/journal.ppat.1010340.s008
DOI:
10.1371/journal.ppat.1010340.s009
DOI:
10.1371/journal.ppat.1010340.s010
DOI:
10.1371/journal.ppat.1010340.s011
DOI:
10.1371/journal.ppat.1010340.s012
DOI:
10.1371/journal.ppat.1010340.s013
DOI:
10.1371/journal.ppat.1010340.s014
DOI:
10.1371/journal.ppat.1010340.s015
DOI:
10.1371/journal.ppat.1010340.s016
DOI:
10.1371/journal.ppat.1010340.s017
DOI:
10.1371/journal.ppat.1010340.s018
DOI:
10.1371/journal.ppat.1010340.s019
DOI:
10.1371/journal.ppat.1010340.s020
DOI:
10.1371/journal.ppat.1010340.s021
DOI:
10.1371/journal.ppat.1010340.s022
DOI:
10.1371/journal.ppat.1010340.s023
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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