In:
Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2014, No. 10 ( 2014-10), p. pdb.prot084145-
Abstract:
We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca 2+ indicators, this optical fiber and its associated fluorescence detection system can be used for the in vivo recording of brain Ca 2+ signals from a local cluster of coactive neurons. The fiber can also be used for the optogenetic stimulation of light-activated ion channels, such as channelrhodopsin-2, allowing the monitoring of local calcium signals evoked by optogenetic stimulation.
Type of Medium:
Online Resource
ISSN:
1940-3402
,
1559-6095
DOI:
10.1101/pdb.prot084145
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2014
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