In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2021-10-25), p. e1009972-
Abstract:
Transcriptional profiling is a powerful tool to investigate and detect human diseases. In this study, we used bulk RNA-sequencing (RNA-Seq) to compare the transcriptomes in skin lesions of leprosy patients or controls affected by other dermal conditions such as granuloma annulare, a confounder for paucibacillary leprosy. We identified five genes capable of accurately distinguishing multibacillary and paucibacillary leprosy from other skin conditions. Indoleamine 2,3-dioxygenase 1 ( IDO1 ) expression alone was highly discriminatory, followed by TLR10 , BLK , CD38 , and SLAMF7 , whereas the HS3ST2 and CD40LG mRNA separated multi- and paucibacillary leprosy. Finally, from the main differentially expressed genes (DEG) and enriched pathways, we conclude that paucibacillary disease is characterized by epithelioid transformation and granuloma formation, with an exacerbated cellular immune response, while multibacillary leprosy features epithelial-mesenchymal transition with phagocytic and lipid biogenesis patterns in the skin. These findings will help catalyze the development of better diagnostic tools and potential host-based therapeutic interventions. Finally, our data may help elucidate host-pathogen interplay driving disease clinical manifestations.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009972
DOI:
10.1371/journal.ppat.1009972.g001
DOI:
10.1371/journal.ppat.1009972.g002
DOI:
10.1371/journal.ppat.1009972.g003
DOI:
10.1371/journal.ppat.1009972.g004
DOI:
10.1371/journal.ppat.1009972.g005
DOI:
10.1371/journal.ppat.1009972.g006
DOI:
10.1371/journal.ppat.1009972.g007
DOI:
10.1371/journal.ppat.1009972.s001
DOI:
10.1371/journal.ppat.1009972.s002
DOI:
10.1371/journal.ppat.1009972.s003
DOI:
10.1371/journal.ppat.1009972.s004
DOI:
10.1371/journal.ppat.1009972.s005
DOI:
10.1371/journal.ppat.1009972.s006
DOI:
10.1371/journal.ppat.1009972.s007
DOI:
10.1371/journal.ppat.1009972.s008
DOI:
10.1371/journal.ppat.1009972.s009
DOI:
10.1371/journal.ppat.1009972.s010
DOI:
10.1371/journal.ppat.1009972.s011
DOI:
10.1371/journal.ppat.1009972.s012
DOI:
10.1371/journal.ppat.1009972.s013
DOI:
10.1371/journal.ppat.1009972.s014
DOI:
10.1371/journal.ppat.1009972.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
Permalink