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  • 1
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    Online Resource
    Seroprevalence of Specific Antibodies to Toxoplasma gondii, Neospora caninum, and Brucella spp. in Sheep and Goats in Egypt
    MDPI AG ; 2022
    In:  Animals Vol. 12, No. 23 ( 2022-11-28), p. 3327-
    Details
    In: Animals, MDPI AG, Vol. 12, No. 23 ( 2022-11-28), p. 3327-
    Abstract: Toxoplasmosis, neosporosis, and brucellosis are devastating diseases causing infectious abortion and, therefore, substantial economic losses in farm animals. Toxoplasmosis and neosporosis are caused by the intracellular protozoan parasites Toxoplasma gondii (T. gondii) and Neospora caninum (N. caninum), respectively. Brucellosis is a bacterial disease caused by numerous Brucella species in multiple hosts. Toxoplasmosis and brucellosis are also considered foodborne zoonotic diseases. In the current study, specific antibodies to T. gondii and N. caninum, in addition to those to Brucella spp., were detected to gain a better understanding of the epidemiological situation for these three pathogens. Sheep and goat sera from Egypt (n = 360) of animals with and without a history of abortion were tested using commercial ELISAs. Seropositivity rates of 46.1%, 11.9%, and 8.6% for T. gondii, N. caninum, and Brucella spp., respectively, were revealed. Mixed infections with T. gondii and Brucella spp. (4.4%), T. gondii and N. caninum (4.2%), N. caninum and Brucella spp. (1.4%), and even some triple infections (0.6%) have been observed. Animals with a history of abortion had a significantly higher seroprevalence for Brucella spp. infection than those without abortion (12.6%; 28/222 vs. 2.2%; 3/138) (p = 0.0005; Odds ratio = 1.9–21.8), while none of the other pathogens showed a similar effect. This result suggests brucellosis as a possible cause of abortion in the study population. However, the high seroprevalence for T. gondii and N. caninum revealed in our study warrants further investigations.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    URL: Article
    DOI: 10.3390/ani12233327
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 2
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    Seroprevalence of Toxoplasma gondii and Neospora caninum in camels recently imported to Egypt from Sudan and a global systematic review
    Frontiers Media SA ; 2022
    In:  Frontiers in Cellular and Infection Microbiology Vol. 12 ( 2022-11-14)
    Details
    In: Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 12 ( 2022-11-14)
    Abstract: Toxoplasma gondii and Neospora caninum are closely related intracellular protozoan parasites of medical and veterinary concern by causing abortions and systemic illness. Limited or ambiguous data on the prevalence of T. gondii and N. caninum in camels triggered us to conduct this study. Methods Camels (n = 460) recently imported from Sudan and destined mainly for human consumption, were tested for specific antibodies against these protozoans using commercially available ELISAs. From the two only quarantine stations for camels from Sudan, 368 camels were sampled between November 2015 and March 2016 in Shalateen, Red Sea governorate, and 92 samples were collected between September 2018 and March 2021 from Abu Simbel, Aswan governorate. Results & amp; Discussion Overall, seropositive rates in camels were 25.7%, 3.9% and 0.8% for T. gondii, N. caninum and mixed infection, respectively. However, marked differences were found between the two study sites and/or the two sampling periods: For T. gondii, a higher rate of infection was recorded in the Red Sea samples (31.5%, 116/368; odds ratio 20.7, 5.0-85.6; P & lt;0.0001) than in those collected in Aswan (2.2%, 2/92). The opposite was found for N. caninum with a lower rate of infection in the Red Sea samples (0.82%, 3/368; odds ratio 23.7, 6.7-83.9; P & lt;0.0001) than in the samples from Aswan (16.3%, 15/92). Additionally, our systematic review revealed that the overall published seroprevalence of T. gondii and N. caninum was 28.6% and 14.3% in camels worldwide, respectively. To the best of our knowledge, this study provides the first record of seroprevalence of both T. gondii and N. caninum in recently imported camels kept under quarantine conditions before delivery to other Egyptian cities and regions. In addition, our review provides inclusive data on the prevalence of T. gondii and N. caninum in camel globally. This knowledge provides basic data for the implementation of strategies and control measures against neosporosis and toxoplasmosis.
    Type of Medium: Online Resource
    ISSN: 2235-2988
    URL: Article
    DOI: 10.3389/fcimb.2022.1042279
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2619676-1
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  • 3
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    Comparative study on Toxoplasma gondii dense granule protein 7, peroxiredoxin 1 and 3 based on bioinformatic analysis tools
    German Multidisciplinary Publishing Center ; 2022
    In:  German Journal of Microbiology Vol. 2, No. 1 ( 2022), p. 30-38
    Details
    In: German Journal of Microbiology, German Multidisciplinary Publishing Center, Vol. 2, No. 1 ( 2022), p. 30-38
    Abstract: Toxoplasmosis remains a devastating protozoan disease induced by Toxoplasma gondii (T. gondii) that induces extreme hazards in both medical and veterinary fields. Our previous studies revealed the high immunogenicity and antigenicity of T. gondii peroxiredoxin (TgPrx) 1, 3, and TgGRA7. Herein, the comparison of TgPrx1, TgPrx3, and TgGRA7 was conducted using bioinformatics analysis tools. In this computational comparison, the physico-chemical, morphometric, immunogenic, and antigenic properties were analyzed. Analyses of complete coding sequences showed the probability of signal peptides and transmembrane domains only in the case of TgGRA7. NetPhos server-based prediction revealed 23, 11, and 39 phosphorylation sites in TgGRA7, TgPrx1, and TgPrx3 proteins, respectively. The secondary structure of TgGRA7, TgPrx1, and TgPrx3 proteins were analyzed by PSIPRED servers. The percentage of the random coil and alpha-helix amino acids was higher in TgGRA7 (99.15%), followed by TgPrx3 (85.87%) and TgPrx1 (77.55%). The antigenic epitopes of the protein were predicted by analyzing the features of the IEDB server. The linear B-cell epitope regions prediction of TgGRA7 showed the maximum estimated length (118 amino acid residues). In addition, antigenicity and hydrophilicity index showed similar tendencies among the three tested proteins, TgGRA7, TgPrx1, and TgPrx3. Thus, the current computational analyses represented TgGRA7, TgPrx1, and TgPrx3 proteins as efficient diagnostic and vaccine candidates suggesting further research and assessments. Additional validation of bioinformatic analysis tools in predicting potent diagnostic and vaccine antigens will greatly contribute to the success of control policies against T. gondii and other infectious agents.
    Type of Medium: Online Resource
    ISSN: 2749-0149
    URL: Issue
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.51585/gjm.2022
    DOI: 10.51585/gjm.2022.1
    DOI: 10.51585/gjm
    DOI: 10.51585/gjm.2022.1.0013
    Language: English
    Publisher: German Multidisciplinary Publishing Center
    Publication Date: 2022
    detail.hit.zdb_id: 3074357-6
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  • 4
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    Vaccination with Neospora GRA6 Interrupts the Vertical Transmission and Partially Protects Dams and Offspring against Neospora caninum Infection in Mice
    MDPI AG ; 2021
    In:  Vaccines Vol. 9, No. 2 ( 2021-02-15), p. 155-
    Details
    In: Vaccines, MDPI AG, Vol. 9, No. 2 ( 2021-02-15), p. 155-
    Abstract: Vaccination is the mainstay of preventative measures for numerous infectious diseases. Neospora caninum infection induces storms of abortion in pregnant cows and ewes, resulting in drastic economic losses because of fetal losses and culling of the dams. Herein, we evaluated the potential of recombinant protein of N. caninum dense granule protein 6 fused with glutathione-S-transferase (NcGRA6+GST) as a vaccine candidate against neosporosis in a pregnant mouse model. The protective efficacy was investigated by subcutaneous inoculation of BALB/c mice with recombinant NcGRA6+GST (25 pmol), and GST alone (25 pmol) or phosphate-buffered saline (PBS) as the controls. This study revealed the partial ability of NcGRA6+GST to protect the dams and offspring from N. caninum infection during the critical period of pregnancy. This ability was revealed by higher survival rate and lower parasite burden in brains of offspring of the NcGRA6+GST-immunized group in comparison with the control groups. In addition, mouse dams from NcGRA6+GST-immunized groups exhibited lower clinical score and minimum alteration in body weight in comparison with PBS or GST groups after challenge with N. caninum tachyzoites. Taken together, our results suggest the efficacy of recombinant NcGRA6 for interrupting the vertical transmission of N. caninum in mice by reducing the severity of infections in dams and offspring.
    Type of Medium: Online Resource
    ISSN: 2076-393X
    URL: Article
    DOI: 10.3390/vaccines9020155
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2703319-3
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  • 5
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    Data_Sheet_1.PDF
    Frontiers Media SA ; 2022
    In:  Frontiers in Public Health Vol. 10 ( 2022-12-7)
    Details
    In: Frontiers in Public Health, Frontiers Media SA, Vol. 10 ( 2022-12-7)
    Abstract: Fascioliasis is a parasitic foodborne disease caused by the liver flukes, Fasciola hepatica and F. gigantica . Such parasites cause serious illness in numerous domestic animals and also in humans. Following infection, the parasite secretes a variety of molecules that immediately interact with the host immunity to establish successful infection. These molecules include cathepsin L peptidase 1 (CatL1); the highly investigated diagnostic and vaccine antigens using various animal models. However, a few studies have analyzed the potentials of FhCatL1 as a diagnostic or vaccine antigen using bioinformatic tools and much less for FgCatL1. The present study provides inclusive and exclusive information on the physico-chemical, antigenic and immunogenic properties of F. hepatica cathepsin L1 (FhCatL1) protein using multiple bioinformatic analysis tools and several online web servers. Also, the validation of our employed available online servers was conducted against a huge collection of previously published studies focusing on the properties of FhCatL1as a diagnostic and vaccine antigen. Methods For this purpose, the secondary, tertiary, and quaternary structure of FhCatL1 protein were also predicted and analyzed using the SWISS-MODEL server. Validation of the modeled structures was performed by Ramachandran plots. The antigenic epitopes of the protein were predicted by IEDB server. Results and discussion Our findings revealed the low similarity of FhCatL1 with mammalian CatL1, lacking signal peptides or transmembrane domain, and the presence of 33 phosphorylation sites. Also, the containment of FhCatL1 for many topological, physico-chemical, immunological properties that favored its function of solubility and interaction with the immune components were reported. In addition, the earlier worldwide reports documented the high efficacy of FhCatL1 as a diagnostic and vaccine antigen in different animals. Altogether, FhCatL1 is considered an excellent candidate for using in commercialized diagnostic assays or vaccine products against fascioliasis in different animal species. Our assessment also included FgCatL1 and reported very similar findings and outputs to those of FhCatL1.
    Type of Medium: Online Resource
    ISSN: 2296-2565
    URL: Article
    URL: Article
    DOI: 10.3389/fpubh.2022.1054502
    DOI: 10.3389/fpubh.2022.1054502.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2711781-9
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  • 6
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    In vitro regulation of gene expression of pregnancy-associated proteins and cytokines in bovine endometrial epithelial cells and bovine trophoblastic cells by infection with Neospora caninum
    Elsevier BV ; 2024
    In:  Parasitology International Vol. 101 ( 2024-08), p. 102898-
    Details
    In: Parasitology International, Elsevier BV, Vol. 101 ( 2024-08), p. 102898-
    Type of Medium: Online Resource
    ISSN: 1383-5769
    URL: Article
    DOI: 10.1016/j.parint.2024.102898
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 1498553-6
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  • 7
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    Data_Sheet_2.XLSX
    Frontiers Media SA ; 2023
    In:  Frontiers in Microbiology Vol. 13 ( 2023-1-10)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2023-1-10)
    Abstract: C-X-C motif chemokine receptor 3 (CXCR3) is an important receptor controlling the migration of leukocytes, although there is no report regarding its role in Neospora caninum infection. Herein, we investigated the relevance of CXCR3 in the resistance mechanism to N. caninum infection in mice. Wild-type (WT) C57BL/6 mice and CXCR3-knockout (CXCR3KO) mice were used in all experiments. WT mice displayed a high survival rate (100%), while 80% of CXCR3KO mice succumbed to N. caninum infection within 50 days. Compared with WT mice, CXCR3KO mice exhibited significantly lower body weights and higher clinical scores at the subacute stage of infection. Flow cytometric analysis revealed CXCR3KO mice as having significantly increased proportions and numbers of CD11c-positive cells compared with WT mice at 5 days post infection (dpi). However, levels of interleukin-6 and interferon-γ in serum and ascites were similar in all groups at 5 dpi. Furthermore, no differences in parasite load were detected in brain, spleen, lungs or liver tissue of CXCR3KO and WT mice at 5 and 21 dpi. mRNA analysis of brain tissue collected from infected mice at 30 dpi revealed no changes in expression levels of inflammatory response genes. Nevertheless, the brain tissue of infected CXCR3KO mice displayed significant necrosis and microglial activation compared with that of WT mice at 21 dpi. Interestingly, the brain tissue of CXCR3KO mice displayed significantly lower numbers of FoxP3 + cells compared with the brain tissue of WT mice at 30 dpi. Accordingly, our study suggests that the lack of active regulatory T cells in brain tissue of infected CXCR3KO mice is the main cause of these mice having severe necrosis and lower survival compared with WT mice. Thus, CXCR3 + regulatory T cells may play a crucial role in control of neosporosis.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2022.1045106
    DOI: 10.3389/fmicb.2022.1045106.s001
    DOI: 10.3389/fmicb.2022.1045106.s002
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2587354-4
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  • 8
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    Prevalence of Neospora caninum and Toxoplasma gondii Antibodies and DNA in Raw Milk of Various Ruminants in Egypt
    MDPI AG ; 2022
    In:  Pathogens Vol. 11, No. 11 ( 2022-11-07), p. 1305-
    Details
    In: Pathogens, MDPI AG, Vol. 11, No. 11 ( 2022-11-07), p. 1305-
    Abstract: The prevalence of Neospora caninum and Toxoplasma gondii antibodies in raw milk samples was estimated in different ruminants and Egyptian governorates. Of 13 bulk milk samples tested by ELISA, five (38.5%) were positive for antibodies to N. caninum, and two samples were additionally positive for antibodies to T. gondii, resulting in a seroprevalence of 15.4% for both T. gondii and co-infection. In individual milk samples (n = 171) from the same bulks, antibodies to N. caninum were detected in 25.7%, to T. gondii in 14%, and 3.5% had antibodies to both parasites. A strong correlation between the OD values of the bulk samples and of the relevant individual milk samples was found for T. gondii (Pearson r = 0.9759) and moderately strong for N. caninum (Pearson r = 0.5801). Risk factor assessment for individual milk samples revealed that antibodies to T. gondii were significantly influenced by animal species, while no risk factors were detected for N. caninum antibodies. Additionally, DNA of N. caninum was detected in a bulk milk sample of cattle for the first time in Egypt, and DNA of T. gondii was found in bulk milk samples of cattle, sheep and goats. This is the first study in Egypt in which bulk milk samples of different ruminants were tested for the presence of N. caninum and T. gondii antibodies and DNA. Both individual and bulk milk samples are useful tools for monitoring antibody response to N. caninum and T. gondii infections in different ruminants in Egypt.
    Type of Medium: Online Resource
    ISSN: 2076-0817
    URL: Article
    DOI: 10.3390/pathogens11111305
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2695572-6
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  • 9
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    Development and evaluation of the first immunochromatographic test that can detect specific antibodies against Cryptosporidium parvum
    Elsevier BV ; 2018
    In:  Acta Tropica Vol. 185 ( 2018-09), p. 349-356
    Details
    In: Acta Tropica, Elsevier BV, Vol. 185 ( 2018-09), p. 349-356
    Type of Medium: Online Resource
    ISSN: 0001-706X
    URL: Article
    DOI: 10.1016/j.actatropica.2018.06.019
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2018
    detail.hit.zdb_id: 1495527-1
    detail.hit.zdb_id: 2709742-0
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  • 10
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    Neospora GRA6 possesses immune-stimulating activity and confers efficient protection against Neospora caninum infection in mice
    Elsevier BV ; 2019
    In:  Veterinary Parasitology Vol. 267 ( 2019-03), p. 61-68
    Details
    In: Veterinary Parasitology, Elsevier BV, Vol. 267 ( 2019-03), p. 61-68
    Type of Medium: Online Resource
    ISSN: 0304-4017
    URL: Article
    DOI: 10.1016/j.vetpar.2019.02.003
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2019
    detail.hit.zdb_id: 1498947-5
    SSG: 12
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