In:
Protein & Peptide Letters, Bentham Science Publishers Ltd., Vol. 30, No. 6 ( 2023-06), p. 506-512
Kurzfassung:
Poly (ethylene terephthalate) (PET) is one of the most abundant polyester
materials used in daily life and it is also one of the main culprits of environmental pollution. ICCG (F243I/D238C/S283C/Y127G) is an enzyme that performs four modifications on the leaf branch
compost keratase (LCC). It shows excellent performance in the hydrolysis of PET and has a great potential in further applications. Methods: Here, we used ICCG to degrade PET particles of various sizes and use the density of attack
sites (Γattack) and kinetic parameters to evaluate the effect of particle size on enzyme degradation efficiency. We are surprised to observe that there is a certain relationship between Km and Γattack. In
order to further confirm the relationship, we obtained three different enzymes (Y95K, M166S and H218S) by site-directed mutagenesis on the basis of ICCG. Result: The results confirmed that there was a negative correlation between Km and Γattack. In
addition, we also found that increasing the affinity between the enzyme and the substrate does not necessarily lead to the increase of degradation rate. Conclusion: These findings show that the granulation of PET and the selection of appropriate
particle size are helpful to improve its industrial application value. At the same time, additional protein engineering to increase ICCG performance is realistic, but it can’t be limited to enhance the
affinity between enzyme and substrate.
Materialart:
Online-Ressource
ISSN:
0929-8665
DOI:
10.2174/0929866530666230509141807
Sprache:
Englisch
Verlag:
Bentham Science Publishers Ltd.
Publikationsdatum:
2023
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