In:
Neuroendocrinology, S. Karger AG, Vol. 99, No. 3-4 ( 2014), p. 178-189
Kurzfassung:
Kisspeptin (KISS1) and its receptor (KISS1r) are essential for normal reproductive function in many species, but the role of kiss1/kiss1r signalling in the dog has not yet been elucidated. The aims of this study were to identify the canine 〈 i 〉 kiss1 〈 /i 〉 and 〈 i 〉 kiss1r 〈 /i 〉 genes and to determine gonadotrophin and oestradiol stimulatory activity of KP-10, the shortest biologically active form of KISS1. Canine 〈 i 〉 kiss1 〈 /i 〉 and 〈 i 〉 kiss1r 〈 /i 〉 genes were localized by comparing the reference dog genome with relevant human cDNA sequences, using BLASTn software. The amino acid sequence of canine KP-10 (YNWN 〈 b 〉 V 〈 /b 〉 FGLR 〈 b 〉 Y 〈 /b 〉 ) differs at two positions from human KP-10 (YNWN 〈 b 〉 S 〈 /b 〉 FGLR 〈 b 〉 F 〈 /b 〉 ). A single bolus of canine KP-10 was administered intravenously to anoestrous Beagle bitches in dosages of 0, 0.1, 0.2, 0.3, 0.5, 1, 5, 10, and 30 μg/kg. Blood samples were collected before and after canine KP-10 administration for the measurement of plasma luteinizing hormone (LH, all doses), follicle-stimulating hormone (FSH) and oestradiol (1-30 μg/kg). From 0.2 μg/kg onwards, canine KP-10 resulted in a rapid and robust rise in plasma LH concentration (max. at 10 min). KP-10 also resulted in a rapid and robust rise in plasma FSH concentration (max. at 10-20 min). Plasma oestradiol concentration increased significantly after dosages of 1, 5, and 10 μg/kg and reached a maximum at 60-90 min. In conclusion, canine KP-10 is a potent kisspeptin which elicits robust gonadotrophin and oestradiol responses in anoestrous bitches, suggesting that canine kiss1/kiss1r are cogent targets for modulating reproduction in dogs.
Materialart:
Online-Ressource
ISSN:
0028-3835
,
1423-0194
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
2014
ZDB Id:
1483028-0
Permalink