ISSN:
1573-4986
Keywords:
confocal microscopy
;
macrophage lineage
;
neoglycoproteins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Myelomonocytic lineage cells express anM r 60 000 mannose specific lectin, MR60 (Pimpaneauet al. (1991),Carbohydr Res 213: 95–108). Under non-reducing conditions, this protein migrates as a 120 000 protein. MR60 does not contain anyN-glycan moiety cleavable by the action ofN-glycanase. MR60 induces a sugar selective aggregation of beads coated with glycosylated albumin: beads bearing α-d-mannosyl residues are aggregated while beads bearing α-d-glucosyl residues are not. A monoclonal antibody Lec101B, specific for MR60, recognizes a singleM r 60 000 protein by Western blotting. This monoclonal antibody does not label the cell surface of cells expressing MR60, but decorates intracellular vesicles upon permeabilization of these cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731206
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