In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_1 ( 2014-01-01), p. 107-115
Abstract:
Two marine bacteria, designated strains MBE#61 T and MBE#74 T , were isolated from a piece of sunken bamboo in the marine environment in Japan. Both of these strains were Gram-stain-negative, but had different cell shapes: MBE#61 T was spiral, whereas MBE#74 T was rod-shaped. The temperature, pH and salt concentration ranges for growth of strain MBE#61 T were 4–38 °C (optimal at 32 °C), pH 4.5–11.0 (optimal at pH 7.0–8.0) and 1–11 % (optimal at 2 %) NaCl, whereas those of strain MBE#74 T were 4–36 °C (optimal at 30 °C), pH 4.0–10.5 (optimal at pH 7.0–8.0) and 1–12 % (optimal at 4 %) NaCl. Phylogenetic analysis based on partial 16S rRNA gene sequences revealed that both strains belong to the genus Thalassospira within the class Alphaproteobacteria . Similarity between the 16S rRNA gene sequence of strain MBE#61 T and those of the type strains of species of the genus Thalassospira was 97.5–99.0 %, and that of strain MBE#74 T was 96.9–98.6 %; these two isolates were most closely related to Thalassospira lucentensis QMT2 T . However, the DNA–DNA hybridization values between T. lucentensis QMT2 T and strain MBE#61 T or MBE#74 T were only 16.0 % and 7.1 %, respectively. The DNA G+C content of strain MBE#61 T was 54.4 mol%, and that of strain MBE#74 T was 55.9 mol%. The predominant isoprenoid quinone of the two strains was Q-10 (MBE#61 T , 97.3 %; MBE#74 T , 93.5 %). The major cellular fatty acids of strain MBE#61 T were C 18 : 1 ω7 c (31.1 %), summed feature 3 comprising C 16 : 0 ω7 c /iso-C 15 : 0 2-OH (26.1 %) and C 16 : 0 (20.9 %); those of strain MBE#74 T were C 16 : 0 (26.2 %), C 17 : 0 cyclo (19.9 %) and C 18 : 1 ω7 c (12.1 %). On the basis of these results, strain MBE#61 T and strain MBE#74 T are considered to represent novel species of the genus Thalassospira , for which names Thalassospira alkalitolerans sp. nov. and Thalassospira mesophila sp. nov. are proposed. The type strains are MBE#61 T ( = JCM 18968 T = CECT 8273 T ) and MBE#74 T ( = JCM 18969 T = CECT 8274 T ), respectively. An emended description of the genus Thalassospira is also proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.056028-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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