In:
Cancer Science, Wiley, Vol. 105, No. 11 ( 2014-11), p. 1411-1420
Abstract:
CD 24 is a heavily glycosylated cell surface protein that is expressed in putative stem cells and is overexpressed in various human malignancies, yet the significant roles of CD 24 in gastric cancer development are still elusive. We investigated the involvement of CD 24 in gastric cancer aggressiveness, which is attributed to its heterogeneity. Cultured gastric cancer cells showed diverse expression patterns in CD 24, whereas other defined cell surface markers, such as CD 44 and CD 133, were homogenous. Purely sorted CD 24‐negative gastric cancer cells showed strong alteration into the CD 24‐positive cell type in an autochthonous manner, and reached to steady expression levels. Our clinicopathological study revealed that CD 24 positivity was an independent prognostic factor in both intestinal and diffuse types of gastric cancer. CD 24 expression was correlated with the advanced stages, invasiveness, and lymph node metastasis of gastric cancer. Silencing of CD 24 in cultured cells significantly decreased cell migration and invasion. Hypoxic treatment upregulated CD 24 expression, and simultaneously induced cell motility and invasion of gastric cancer cells. Hypoxic treatment‐induced CD 24 expression was significantly attenuated by knockdown of hypoxia‐inducible transcription factors. These data suggest that CD 24‐negative cells are capable of gaining cell motility and invasiveness through the induction of CD 24, which is mediated by hypoxia. CD 24 would be an attractive marker to define not only the heterogeneity but also the aggressiveness of gastric cancer cells. The mechanisms by which hypoxia induces CD 24 expression would also be a potential therapeutic target for gastric cancer.
Type of Medium:
Online Resource
ISSN:
1347-9032
,
1349-7006
DOI:
10.1111/cas.2014.105.issue-11
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2115647-5
detail.hit.zdb_id:
2111204-6
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