In:
Developmental Dynamics, Wiley, Vol. 251, No. 12 ( 2022-12), p. 1934-1951
Abstract:
Knockdown of zebrafish p120 catenin‐δ1 with a morpholino blocks migration of presomitic mesoderm and extension of the dorsal axis. We compared rescue of morphology by injecting mouse p120 catenin‐δ1‐3A (CTNND1‐3A) mRNAs mutated to phosphomimetic (E) and nonphosphorylatable (F) versions of tyrosine residues. Mutations to Y228F, Y228E, Y217F, or Y335E rescued morphology; mutation to Y217E, or Y335F mRNAs only partially rescued; and dual mutation to Y228E‐Y217E blocked rescue. Immunopurification showed Y228F mutant proteins preferentially binding to Rac1, potentially promoting cell migration, and the Y228E protein preferentially binding with E‐cadherin increasing adhesion. Both Y228F and Y335F bound strongly to VAV2. We show the importance of phosphorylation state for regulating adhesion and mesodermal cell migration in early vertebrate morphogenesis.
Type of Medium:
Online Resource
ISSN:
1058-8388
,
1097-0177
DOI:
10.1002/dvdy.v251.12
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
1473797-8
SSG:
12
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