In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 10 ( 2022-10-18), p. e1010909-
Kurzfassung:
Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. Here, we explore the hypothesis that combinatorial interactions may expand the functional landscape of the viral proteome. We show that the proteins encoded by a plant-infecting DNA virus, the geminivirus tomato yellow leaf curl virus (TYLCV), physically associate with one another in an intricate network, as detected by a number of protein-protein interaction techniques. Importantly, our results indicate that intra-viral protein-protein interactions can modify the subcellular localization of the proteins involved. Using one particular pairwise interaction, that between the virus-encoded C2 and CP proteins, as proof-of-concept, we demonstrate that the combination of viral proteins leads to novel transcriptional effects on the host cell. Taken together, our results underscore the importance of studying viral protein function in the context of the infection. We propose a model in which viral proteins might have evolved to extensively interact with other elements within the viral proteome, enlarging the potential functional landscape available to the pathogen.
Materialart:
Online-Ressource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010909
DOI:
10.1371/journal.ppat.1010909.g001
DOI:
10.1371/journal.ppat.1010909.g002
DOI:
10.1371/journal.ppat.1010909.g003
DOI:
10.1371/journal.ppat.1010909.g004
DOI:
10.1371/journal.ppat.1010909.g005
DOI:
10.1371/journal.ppat.1010909.g006
DOI:
10.1371/journal.ppat.1010909.s001
DOI:
10.1371/journal.ppat.1010909.s002
DOI:
10.1371/journal.ppat.1010909.s003
DOI:
10.1371/journal.ppat.1010909.s004
DOI:
10.1371/journal.ppat.1010909.s005
DOI:
10.1371/journal.ppat.1010909.s006
DOI:
10.1371/journal.ppat.1010909.s007
DOI:
10.1371/journal.ppat.1010909.s008
DOI:
10.1371/journal.ppat.1010909.s009
DOI:
10.1371/journal.ppat.1010909.s010
DOI:
10.1371/journal.ppat.1010909.s011
DOI:
10.1371/journal.ppat.1010909.s012
DOI:
10.1371/journal.ppat.1010909.s013
DOI:
10.1371/journal.ppat.1010909.s014
DOI:
10.1371/journal.ppat.1010909.s015
DOI:
10.1371/journal.ppat.1010909.s016
DOI:
10.1371/journal.ppat.1010909.s017
DOI:
10.1371/journal.ppat.1010909.s018
DOI:
10.1371/journal.ppat.1010909.r001
DOI:
10.1371/journal.ppat.1010909.r002
DOI:
10.1371/journal.ppat.1010909.r003
DOI:
10.1371/journal.ppat.1010909.r004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2022
ZDB Id:
2205412-1
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