In:
The American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, Vol. 104, No. 4 ( 2021-04-07), p. 1326-1334
Kurzfassung:
Isolates from 475 cutaneous leishmaniasis (CL) patients from three endemic regions were studied by three typing techniques. The molecular analysis from lesion scrapings based on hsp70 PCR-restriction fragment length polymorphism (RFLP) showed that 78.1% (371/475) restriction patterns corresponded to Leishmania ( Viannia ) panamensis , 19% (90/475) to Leishmania ( Viannia ) guyanensis , and 3.0% (14/475) to Leishmania ( Viannia ) braziliensis . Promastigotes isolated by culture from lesions of 228 patients (48.0%, 228/475) were identified by multi-locus enzyme electrophoresis. Of them, 95.2% (217/228) were typified as L. ( V. ) panamensis , 1.3% (3/228) as L. ( V. ) guyanensis , 2.2% (5/228) as L. ( V. ) braziliensis , and 1.3% (3/228) as hybrids ( L. [ V. ] braziliensis / L. [ V. ] panamensis ) . However, a partial sequencing analysis of the hsp70 gene from 77 selected samples showed 16.9% (13/77) typified as L. ( V. ) panamensis , 68.8% (53/77) as Leishmania ( V .) sp., 1, 3.9% (3/77) as L. ( V. ) guyanensis , 1.3% (1/77) as L. ( V. ) braziliensis outlier, 2.6% (2/77) as Leishmania ( Viannia ) naiffi , 2.6% as (2/77) Leishmania ( V. ) sp., and 2 and 3.9% (3/77) hybrid isolates of L. ( V. ) braziliensis / L. ( V. ) guyanensis . These results confirm L. ( V. ) panamensis as the predominant species and cause of CL lesions in Panama and that L. ( V. ) guyanensis , L. ( V. ) braziliensis , and L. ( V. ) naiffi are circulating to a lower degree. Furthermore, the determination of parasite isolates belonging to atypical clusters and hybrid isolates suggests the circulation of genetic variants with important implications for the epidemiology and clinical follow-up of CL in Panama. No evidence of the existence of parasites of the Leishmania ( Leishmania ) mexicana complex in Panamanian territory was found in this study.
Materialart:
Online-Ressource
ISSN:
0002-9637
,
1476-1645
DOI:
10.4269/ajtmh.20-1336
Sprache:
Unbekannt
Verlag:
American Society of Tropical Medicine and Hygiene
Publikationsdatum:
2021
ZDB Id:
1491674-5
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