In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-5-26)
Abstract:
Pathogenesis-related (PR) proteins play important roles in plant defense response and systemic acquired resistance (SAR). PR1 has antifungal activity against many plant pathogens. In our previous study, RNA sequencing (RNA-seq) was conducted on resistant wheat line TcLr19 and sensitive wheat cultivar Chinese Spring inoculated with Puccinia triticina ( Pt ) race PHNT. In this study, seven salicylic acid (SA)-induced TaPR1 genes involved in plant disease resistance were found in the RNA-seq library. Quantitative PCR (qPCR) results showed that TaPR1-4 was most induced by Pt among these seven TaPR1 genes in the incompatible interaction. Yeast two-hybrid (Y2H) results showed that TaPR1-4 interacted with TaTLP1 via the αIV helix. Protein-mediated phenotyping assays in vivo and antifungal activity in vitro demonstrated that wheat leaves infiltrated with pure TaPR1-4 protein developed significantly less disease compared to control leaves. This effect was correlated with a strong increase in defense gene expression, and resistance activity was dependent on the CAPE1 motif located in the C-terminal region of TaPR1-4. These findings increase current knowledge regarding the interaction of TaPR1 and TaTLP1 and provide new insights on the role of TaPR1 protein in the resistance of wheat to Pt .
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2022.874654
DOI:
10.3389/fpls.2022.874654.s001
DOI:
10.3389/fpls.2022.874654.s002
DOI:
10.3389/fpls.2022.874654.s003
DOI:
10.3389/fpls.2022.874654.s004
DOI:
10.3389/fpls.2022.874654.s005
DOI:
10.3389/fpls.2022.874654.s006
DOI:
10.3389/fpls.2022.874654.s007
DOI:
10.3389/fpls.2022.874654.s008
DOI:
10.3389/fpls.2022.874654.s009
DOI:
10.3389/fpls.2022.874654.s010
DOI:
10.3389/fpls.2022.874654.s011
DOI:
10.3389/fpls.2022.874654.s012
DOI:
10.3389/fpls.2022.874654.s013
DOI:
10.3389/fpls.2022.874654.s014
DOI:
10.3389/fpls.2022.874654.s015
DOI:
10.3389/fpls.2022.874654.s016
DOI:
10.3389/fpls.2022.874654.s017
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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